FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4385377
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA4314450.0241.10417
TCGTGAC4329800.0241.04332
GTGACTG4334100.0240.525634
TGACTGG4334650.0240.426645
TGGGAAA4340750.0239.65459
GACTGGG4357400.0238.832786
CGTGACT4512750.0231.093723
CTGGGAA4513650.0230.423368
TCGAGAC9650.0149.402392
CGAGACT10350.0148.575933
ACGTGAC7450.0141.48192
GACTGGA17650.0141.229456
GTCGTGA8418850.0123.941
ACTCGGA4200.0118.115697
GTGACCG9900.0111.265994
GACTGGC6350.0100.996166
CTGCGAA7150.089.688738
GACGTGA12050.083.48291
ACCGGGA14100.076.373717
TGCGAAA8800.075.621989
Produced by FastQC (version 0.11.5)