FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371003
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTCTGTGGTCGTGATTGCCGTCGGACTTG71440.5210783637964322No Hit
GTCGTGACTGGGAAAACCCTGGGAGTTGGTCGTGATGGTTCGGTCACTTG51180.37330334069290877No Hit
GTCGTGACTGGGAAAACCCTGGGGCGTTGTCGTGATCGAATGGGTACTTG19250.14040815373854032No Hit
GTCGTGACTGGGAAAACCCTGGGCTTCGGTCGTGATTGCAAGACAACTTG16760.12224626787833433No Hit
GTCGTGACTGGGAAAACCCTGGTAACTAGTCGTGAGATGGGAGTCCTTGT15970.11648406312750592No Hit
GTCGTGACTGGGAAAACCCTGGAGCTCTGTCGTGATGATGTGTGAACTTG15500.11305591599726625No Hit
GTCGTGACTGGGAAAACCCTGGGTGGCGGTCGTGATATCGCAGAGACTTG14380.10488671432520572No Hit
GTCGTGACTGGGAAAACCCTGGGCCGTGGTCGTGATAGTGACCGTACTTG14290.10423026061941512No Hit
GTCGTGACTGGGAAAACCCTGGACGCCCGTCGTGATAGCGAGAAGACTTG14090.10277147460654719No Hit
GTCGTGACTGGGAAAACCCTGGTACTTAGTCGTGATAAAATGGAGACTTG13880.10123974929303584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1276150.0241.462222
TGACTGG1287300.0240.400015
GTGACTG1289900.0240.023094
ACTGGGA1290850.0239.63957
GACTGGG1303700.0239.216036
CGTGACT1273000.0238.689153
TGGGAAA1275350.0237.45459
CTGGGAA1322700.0232.496878
ACGGGGA19000.0203.257427
CGTGACA17900.0202.421023
TGACGGG19250.0195.629365
CGTGACG13500.0192.993263
GACGGGG21750.0192.614756
GTGACGG22900.0171.372364
AGGGAAA22300.0168.83599
ACTGGGG15300.0165.372837
TCGTGGC12300.0163.623892
GGCGTGA11550.0159.640641
CGTGGCT13700.0158.332433
TCGGGAC14750.0156.994722
Produced by FastQC (version 0.11.5)