FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4857521
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCCAA50070.10307726924906759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT4784600.0241.41253
TCGTGAC4793750.0241.254552
TGACTGG4790300.0241.163255
GTGACTG4795200.0240.877474
ACTGGGA4806700.0240.04137
GACTGGG4814750.0239.793056
TGGGAAA4949800.0233.082129
CTGGGAA5030350.0229.296888
ACGTGAC5400.0177.266432
ACCGGGA10100.0138.965797
GTCGTGA9355900.0123.566091
CGAGACT16250.099.8471763
TTGTGAC6550.098.045512
TCGAGAC14700.097.2652742
CGTGACC17700.089.615343
CGGGGAA11500.086.2745749
TCGCGAC3700.085.1460952
TGACCGG20350.085.033025
CGTGGCT18900.083.284833
TCGGACT6550.083.2461852
Produced by FastQC (version 0.11.5)