FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213925
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGATCA17520.14432522602302447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1189900.0241.680133
TCGTGAC1193200.0241.23222
TGACTGG1192400.0241.06715
GTGACTG1192700.0241.01294
GACTGGG1198150.0239.799336
ACTGGGA1199250.0239.216357
CTGGGAA1235700.0231.964258
TGGGAAA1253050.0228.70469
ACGTGAC1700.0185.372762
ACCGGGA2800.0151.25077
TCGTGGC5000.0126.053472
GTCGTGA2324550.0123.725211
CGGGGAA3150.0115.229139
CGTGACC3800.0114.759743
ACGGGAA1100.0110.00057
TGCGAAA1950.0105.478979
CGAGACT4150.0105.081213
ACGAGAC706.6174835E-9103.890222
TCGAGAC4550.093.234822
GCGTGGC2650.091.475672
Produced by FastQC (version 0.11.5)