FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1863032
Sequences flagged as poor quality0
Sequence length248
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGACCCTGCCGTCGTGATGCCGCGAGATCAC43850.2353690113750059No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTCGTGATAAAAAAAGATCAC19690.10568793235972329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1745850.0242.07562
GTGACTG1742350.0241.777654
TGACTGG1742100.0241.684375
ACTGGGA1727350.0241.332087
GACTGGG1748750.0241.226336
CGTGACT1735250.0240.526053
TGGGAAA1706950.0240.481389
CTGGGAA1748500.0237.67448
CGTGGCT29600.0235.784973
GTGACGG13300.0229.493734
GTGACAG22700.0229.437594
TCGGGAC6350.0227.197632
CGTGACA22550.0225.677413
TGACAGG24200.0216.665365
CGAGACT2300.0210.72573
GTGGTGA29100.0209.254561
GTGAGTG5250.0205.330144
TGGTGAC27700.0200.89242
TCGTGGC30450.0200.666022
TCGAGAC2550.0199.682362
Produced by FastQC (version 0.11.5)