FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950691
Sequences flagged as poor quality0
Sequence length248
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGACCCTGCCGTCGTGATGCCGCGAGATCAC19850.20879549716995324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC904500.0242.2492
GTGACTG900300.0241.980124
CGTGACT895150.0241.930283
ACTGGGA892150.0241.593777
GACTGGG901400.0241.141366
TGGGAAA883400.0240.698529
TGACTGG905000.0240.489045
CTGGGAA896750.0239.248668
GTGACAG11250.0235.74994
CGTGGCT9600.0229.714683
CGAGACT900.0228.873233
TCGTGGC9000.0227.658832
CGTGACA11450.0227.521043
TTGTGAC500.0218.229172
TGGTGAC9700.0209.979972
TCGAGAC1050.0207.837312
GTGGTGA10200.0205.716611
GTGACGG4200.0204.724094
ACTGGGG9350.0204.528557
CAGGGAA13500.0199.023738
Produced by FastQC (version 0.11.5)