FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301465
Sequences flagged as poor quality0
Sequence length248
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCGCTGGTCCTGATAGCGCGGTCGCAGCG15870.5264292703962318No Hit
GTCGTGACTGGGAAAACCCTGGCCCGCGGTCGTGATTCTAACAAACAGAT10730.35592854891944337No Hit
GTCGTGACTGGGAAAACCCTGGCGCTGGTCGTGATAGCGCGGTCGCAGCG8060.2673610535219677No Hit
GTCGTGACTGGGAAAACCCTGGCCGTGTGTCGTGATCTCCGGGTCCAGAT5710.1894083890335528No Hit
GTCGTGACTGGGAAAACCCTGGCAGTTTGTCGTGATATTAACTTGCAGAT5710.1894083890335528No Hit
GTCGTGACTGGGAAAACCCTGGCCGTGGTCCTGATCTCGCTAAAACAGAT5450.18078383891994093No Hit
GTCGTGACTGGGAAAACCCTGGTTGACAGTCGTGATCAGTATTAACAGAT4720.15656875590864613No Hit
GTCGTGACTGGGAAAACCCTGGCCGTGGTCGTGATCTCGCTAAAACAGAT4510.14960277312457498No Hit
GTCGTGACTGGGAAAACCCTGGTATTTAGTCGTGATGAGTTTAAACAGAT4320.14330021727232017No Hit
GTCGTGACTGGGAAAACCCTGGCGATTTGTCGTGATTGCTAGTTGCAGAT4180.1386562287496061No Hit
GTCGTGACTGGGAAAACCCTGGTGTGCTGTCGTGATGAGTACAGGCAGAT4100.1360025210223409No Hit
GTCGTGACTGGGAAAACCCTGGAACGATGTCGTGATTTGATCGGCCAGAT3940.13069510556781053No Hit
GTCGTGACTGGGAAAACCCTGGCTCTCTGTCGTGATCCAGACGGCCAGAT3720.12339740931783126No Hit
GTCGTGACTGGGAAAACCCTGGCACGGCGTCGTGATGCGAAGAGGCAGAT3690.12240226892010682No Hit
GTCGTGACTGGGAAAACCCTGGTCCTCCGTCGTGATGGGCAGTACCAGAT3510.11643142653376014No Hit
GTCGTGACTGGGAAAACCCTGGTCGCATGTCGTGATGCCATAGGGCAGTC3500.11609971306785198No Hit
GTCGTGACTGGGAAAACCCTGGTCCGCGGTCGTGATGGACGGACCCAGAT3480.11543628613603568No Hit
GTCGTGACTGGGAAAACCCTGGAACGGGGTCGTGATGAAGAGGGCCAGAT3450.11444114573831124No Hit
GTCGTGACTGGGAAAACCCTGGGTTCGTGTCGTGATTGGTACAGGCAGAT3170.10515316869288309No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAG100.00152631242.253893
TCGTGAC248400.0241.496492
TGACTGG253450.0241.074625
GTGACTG253600.0240.97984
CGTGACT245350.0240.871573
TGACGGG5100.0239.799215
ACTGGGA256100.0239.446247
TGGGAAA248450.0239.316029
GACTGGG257700.0239.173546
CTGGGAA258900.0235.87488
GTGACGG5250.0232.947814
ATGATCG5000.0225.146597
TTATCGG1200.0221.918698
GGTGACT9350.0221.52633
ACGGGGA6000.0219.901237
GAGATCG4350.0219.831677
AAGATCG5900.0219.52517
GTGGTGA12000.0217.271681
TAGATCG2400.0216.875087
CGTGACG3750.0216.41353
Produced by FastQC (version 0.11.5)