FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777037
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCCGAGGGTCGTGATACGTATTTACAGTG17700.2277883807334786No Hit
GTCGTGACTGGGAAAACCCTGGAGTCAAGTCGTGATGCACGGACACAGTG13260.17064824454948735No Hit
GTCGTGACTGGGAAAACCCTGGCGACTAGTCGTGATGTGAGGTTTCAGTG13170.16948999854575778No Hit
GTCGTGACTGGGAAAACCCTGGAATTCGGTCGTGATTGAGATTACCAGTG12760.16421354452876763No Hit
GTCGTGACTGGGAAAACCCTGGAGTCTGGTCGTGATGACTCTAGTCAGTG12050.1550762704993456No Hit
GTCGTGACTGGGAAAACCCTGGAACGATGTCGTGATCGCGTCCATCAGTG11700.15057198048484177No Hit
GTCGTGACTGGGAAAACCCTGGCCGGTGGTCGTGATGATTCGTATCAGTG11660.1500572044831842No Hit
GTCGTGACTGGGAAAACCCTGGGATGAAGTCGTGATTAAGTCAACCAGTG11270.14503813846702282No Hit
GTCGTGACTGGGAAAACCCTGGGTCCGGGTCGTGATATAAACGACCAGTG11060.14233556445832052No Hit
GTCGTGACTGGGAAAACCCTGGTAGTGAGTCGTGATTCATAGGACCAGTG10790.13886082644713188No Hit
GTCGTGACTGGGAAAACCCTGGCCAACAGTCGTGATTAAGATTCCCAGTG10780.13873213244671748No Hit
GTCGTGACTGGGAAAACCCTGGTGTCCTGTCGTGATCGCGGTTACCAGTG10720.1379599684442311No Hit
GTCGTGACTGGGAAAACCCTGGCAAAAGGTCGGATCGATCAAATTCAGTG10580.13615825243842958No Hit
GTCGTGACTGGGAAAACCCTGGTGATCGGTCGTGATTAGGAGTATCAGTG10150.1306244104206106No Hit
GTCGTGACTGGGAAAACCCTGGAATGTTGTCGTGATAGGCGGTCCCAGTG9960.1281792244127371No Hit
GTCGTGACTGGGAAAACCCTGGGGTCGCGTCGTGATCAGAAGTCCCAGTG9840.12663489640776437No Hit
GTCGTGACTGGGAAAACCCTGGGCAGATGTCGTGATGATCCTTACCAGTG9710.12496187440237724No Hit
GTCGTGACTGGGAAAACCCTGGCATGAAGTCGTGATATACAATTTCAGTG9650.12418971039989087No Hit
GTCGTGACTGGGAAAACCCTGGATATCTGTCGTATAACCGATTGACAGTG9420.1212297483903598No Hit
GTCGTGACTGGGAAAACCCTGGCATTGCGTCGTGATATAAGTAAACAGTG9380.12071497238870221No Hit
GTCGTGACTGGGAAAACCCTGGTAACATGTCGTGATCCGATCAAACAGTG9380.12071497238870221No Hit
GTCGTGACTGGGAAAACCCTGGGACTCAGTCGTGATTCACACTCCCAGTG9360.12045758438787343No Hit
GTCGTGACTGGGAAAACCCTGGTTCAACGTCGTGATCCGGAGGAACAGTG9310.11981411438580145No Hit
GTCGTGACTGGGAAAACCCTGGAATTCTGTCGTGATGTCAAGTGACAGTG9300.11968542038538704No Hit
GTCGTGACTGGGAAAACCCTGGATACGAGTCGTGATCGCAAGATGCAGTG8840.1137654963663249No Hit
GTCGTGACTGGGAAAACCCTGGGCACACGTCGTGATGTGAGCTTACAGTG8800.11325072036466731No Hit
GTCGTGACTGGGAAAACCCTGGGAAAGGGTCGTGATGGATAACCTCAGTG8800.11325072036466731No Hit
GTCGTGACTGGGAAAACCCTGGTTCTCAGTCGTGATTCAATGGTACAGTG8790.11312202636425293No Hit
GTCGTGACTGGGAAAACCCTGGGCCTTGGTCGTGAAATTTACTTACAGTG8600.11067684035637942No Hit
GTCGTGACTGGGAAAACCCTGGCAAATCGTCGTGATGGTTCAGCCCAGTG8590.11054814635596502No Hit
GTCGTGACTGGGAAAACCCTGGAAGAAGGTCGGAACACCTTCTTCCAGTG8300.10681602034394759No Hit
GTCGTGACTGGGAAAACCCTGGACTAAAGTCGTGATAACGAGAGCCAGTG8250.1061725503418756No Hit
GTCGTGACTGGGAAAACCCTGGTGACCCGTCGTGATATAAGATGACAGTG8220.10578646834063243No Hit
GTCGTGACTGGGAAAACCCTGGGCATGGGTCGTGATCGCTGCTTGCAGTG8160.10501430433814607No Hit
GTCGTGACTGGGAAAACCCTGGCAGTTGGTCGTGATGCGCATGAACAGTG8130.10462822233690287No Hit
GTCGTGACTGGGAAAACCCTGGTGGACCGTCGTGATGCCATGGTGCAGTG8080.10398475233483091No Hit
GTCGTGACTGGGAAAACCCTGGCGTTTGGTCGTGATTGGTAGGAGCAGTG8080.10398475233483091No Hit
GTCGTGACTGGGAAAACCCTGGGGTGCGGTCGTGATCCGCGATGACAGTG7960.10244042432985816No Hit
GTCGTGACTGGGAAAACCCTGGATATACGTCGTGATAACCGACATCAGTG7940.10218303632902938No Hit
GTCGTGACTGGGAAAACCCTGGTGCAATGTCGTGATTTCACAGTCCAGTG7850.10102479032529982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACT700.0242.299223
TCGTGAC739100.0241.42632
ACTGGGA739650.0241.115347
TGGGAAA698550.0239.622289
GTGACTG752950.0238.68544
TGACTGG751600.0238.680625
GACTGGG755550.0236.970416
CGTGGCT5450.0235.630433
CTGGGAA767650.0232.257588
CGTGACT766800.0232.171843
TCGGACT1250.0222.987142
TCGGGAC2650.0219.511542
GTCGGAC1350.0206.562761
CGGACTT1000.0205.954333
TGGGAAC33900.0200.649969
CGAGACT800.0166.580733
CGGACTG453.074092E-10161.532823
GTGACTT4000.0160.43024
GTGGTGA13800.0156.386351
GGTGACT15700.0138.125993
Produced by FastQC (version 0.11.5)