FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541073
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTTTCAAGTCGTGATAGCACAGGGACTTG205861.3358225080836532No Hit
GTCGTGACTGGGAAAACCCTGGATTGGTGTCGTGATGTGAGTAAGACTTG69950.45390451977291146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG1480600.0241.61455
GTGACTG1479950.0241.588064
TCGTGAC1470950.0241.171312
GACTGGG1485550.0241.054186
CGTGACT1466850.0241.052283
ACTGGGA1476900.0240.754177
CTGGGAA1518550.0234.557758
TGGGAAA1430900.0234.283949
CGTGGCT18050.0225.419253
TCGTGGC16450.0218.769882
GTGGTGA25650.0199.048451
GCGTGGC5150.0167.050632
ACTGGGG15550.0165.014827
GGTGACT34350.0148.06473
TGCGAAA1800.0147.929089
GTGGCTG29300.0146.691544
ACTTGGA9250.0143.935657
GTCGTGG26550.0139.315481
TGGTGAC36900.0137.917682
CTTGGAA10000.0131.925848
Produced by FastQC (version 0.11.5)