FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13304
Sequences flagged as poor quality0
Sequence length248
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGTCTAAGTCGTGATCTTAGGTGACAGGA8386.298857486470235No Hit
GTCGTGACTGGGAAAACCCTGGTACCTCGTCGTGATAGGGAGTGACAGAT3522.6458208057727No Hit
GTCGTGACTGGGAAAACCCTGGACTGAGGTCGTGATGAAGAGTACCAGAT2742.0595309681298857No Hit
GTCGTGACTGGGAAAACCCTGGAACCCTGTCGTGATACGAAATTGCAGAT2071.5559230306674685No Hit
GTCGTGACTGGGAAAACCCTGGGGGCCGGTCGTGATAGCGTGCCTCAGAT1631.225195429945881No Hit
GTCGTGACTGGGAAAACCCTGGCCCCTTGTCGTGATAGGTTGAGTCAGAT1611.2101623571858087No Hit
GTCGTGACTGGGAAAACCCTGGCATATTGTCGTGATGTGTACTCCCAGAT1481.1124473842453397No Hit
GTCGTGACTGGGAAAACCCTGGCGCTTTGTCGTGATCTTCGAAGACAGAT1421.0673481659651234No Hit
GTCGTGACTGGGAAAACCCTGGGGCCTGGTCGTGATACATACAAACAGAT920.6915213469633193No Hit
GTCGTGACTGGGAAAACCCTGGACCGTCGTCGTGATAATATTATCCAGAT920.6915213469633193No Hit
GTCGTGACTGGGAAAACCCTGGACTGAGGTCGTGATCCATGACCCCAGAT830.6238725195429946No Hit
GTCGTGACTGGGAAAACCCTGGTTTGTAGTCGTGATTGGGACCAGCAGAT660.49609140108238126No Hit
GTCGTGACTGGGAAAACCCTGGAACTGCGTCGTGATTGGCAGGGTCAGAT490.3683102826217679No Hit
GTCGTGACTGGGAAAACCCGGGGTCTAAGTCGTGATCTTAGGTGACAGGA440.3307276007215875No Hit
GTCGTGACTGGGAAAACCCTGGACTTATGTCGTGATCTGCGCCAGCAGAT430.32321106434155145No Hit
GTCGTGACTGGGAAAACCCTGGTGACAAGTCGTACGAAATCCGTGCGGGT400.30066145520144316No Hit
GTCGTGACTGGGAAAACCCTGGTCTCCTGTCGTGATTTGAAGCTTCAGTC390.2931449188214071No Hit
GTCGTGACTGGGAAAACCCTGGTAGCACGTCGTGATAAGGCCTTGCAGAT370.27811184606133493No Hit
GTCGTGACTGGGGGGGTCTTCGGAAGAGCGTCGTGTAGGGAAAGACAGTC360.27059530968129886Illumina Single End PCR Primer 1 (96% over 27bp)
GTCGTGACTGGGAAAACCCTGGTGTCTCGTCGTGATGTAAAGATCCAGAT330.24804570054119063No Hit
GTCGTGACTGGGAAAACCCTGGCCCTTTGTCGTGATAATGCTCCGCAGAT330.24804570054119063No Hit
GTCGTGACTGGGAAAACCCTGGCTATCCGTCGTGATTCGGCCATCCAGAT320.24052916416115455No Hit
GTCGTGACTGGGAAAACCCTGGGTCTCAGTCGTGATTGCAAGGGGCAGAT320.24052916416115455No Hit
GTCGTGACTGGGAAAACCCTGGATCCCAGTCGTGATTAACGCACACAGAT310.23301262778111848No Hit
GTCGTGACTGGGAAAACCCTGGACCACGGTCGTGATCACCGCGGTCAGAT310.23301262778111848No Hit
GTCGTGACTGGGAAAACCCTGGTCCTGTGTCGTGATACTTGTGAACAGTC280.21046301864101025No Hit
GTCGTGACTGGGAAAACCCTGGATCCGAGTCGTGATTAGGGATGTCAGTA280.21046301864101025No Hit
GTCGTGACTGGGAAAACCCTGGGTGTGGGTCGTGATCTGTGCCCTCAGAT250.187913409500902No Hit
GTCGTGACTGGGAAAATCTTCGGAAGAGCGTCGTGTAGGGAAAGACAGTC250.187913409500902Illumina Single End PCR Primer 1 (96% over 27bp)
GTCGTGACTGGGAAAACCCTGGGAAGGAGTCGTGAAAAAATAGTTCAGAT240.1803968731208659No Hit
GTCGTGACTGGGAAAACCCTGGCTAAGAGTCGTGATCAGTGGGGACAGAT230.17288033674082984No Hit
GTCGTGACTGGGAAAACCCTGGCAGTTTGTCGTGATTAATTCAAACAGAT220.16536380036079376No Hit
GTCGTGACTGGGAAAACCCTGGTCGGAGGTCGTGATCCCAAGTACAGCAT220.16536380036079376No Hit
GTCGTGACTGGGAAAACCCTGGTTCCTAGTCGTGATGATACTGGACACGA200.15033072760072158No Hit
GTCGTGACTGGGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGACAGTC200.15033072760072158Illumina Single End PCR Primer 1 (96% over 29bp)
GTCGTGACTGGGAAAATGTTCGGAAGAGCGTCGTGTAGGGAAAGACAGTC200.15033072760072158Illumina Single End PCR Primer 1 (96% over 26bp)
GTCGTGACTGGGTGGGTCTTCGGAAGAGCGTCGTGTAGGGAAAGACAGTC190.1428141912206855Illumina Single End PCR Primer 1 (96% over 27bp)
GTCGTGACTGGGAAAACCCTGGGATGTGGTCGTGATTAGAGCAAGCAGAT190.1428141912206855No Hit
GTCGTGACTGGGAAAACCCTGGCCGAGAGTCGTGATGCAAATACTCAGAT190.1428141912206855No Hit
GTCGTGACTGGGAAAACCCTGGTTGGTTGTCGTGATGCGGCCATTCAGAT180.13529765484064943No Hit
GTCGTGACTGGGAAAACCCTGGAAACGTGTCGTGATGCAAGTCTGCAGAT180.13529765484064943No Hit
GTCGTGACTGGGAAAACCCTGGCGCACCGTCGTGATGTGCGTACTCAGAT170.12778111846061335No Hit
GTCGTGACTGGGAAAACCCTGGCTCCGCGTCGTGATGCCAATTCTCATAC170.12778111846061335No Hit
GTGTGACTGGGAAAACCCTGGGTTTTCGTCCTGATCTTCAGTGGGCAGAT160.12026458208057728No Hit
GTCGTGACTGGGAAAACCCTGGAACTAGGTCGTGATGCAGCTGCTCACGA160.12026458208057728No Hit
GTCGTGACTGGGGGGGTGTTCGGAAGAGCGTCGTGTAGGGAAAGACAGTC160.12026458208057728Illumina Single End PCR Primer 1 (96% over 26bp)
GTCGTGACTGGGAAAACCCTGGACCAAGGTCGTGATCCCGGACCACAGAT160.12026458208057728No Hit
GTCGTGACTGGGAAAACCCTGGGGGACGGTCGTGATCGTAAGGGACAGAG160.12026458208057728No Hit
GTCGTGACTGGGGGGGACATCGGAAGAGCGTCGTGTAGGGAAAGACAGTC160.12026458208057728Illumina Single End PCR Primer 1 (96% over 29bp)
GTCGTGACTGGGAAAACCCTGGACACCTGTCGTGATCCTGAGAGACAGAT160.12026458208057728No Hit
GTCGTGACTGGGTGGGTGTTCGGAAGAGCGTCGTGTAGGGAAAGACAGTC160.12026458208057728Illumina Single End PCR Primer 1 (96% over 26bp)
GTCGTGACTGGGAAAACCCTGGTGCGGCGTCGTGATTAGAGAGCCCAGAG150.1127480457005412No Hit
GTGGTGACTGGGAAAACCCTGGGTCTAAGTCGTGATCTTAGGTGACAGGA150.1127480457005412No Hit
GTCGTGACAGGGAAAACCCTGGGTCTAAGTCGTGATCTTAGGTGACAGGA140.10523150932050512No Hit
GTCGTGACTGGGAAAACCCTGGCGACACGTCGTGATGTGTAGACACAGAT140.10523150932050512No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)