FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254914
Sequences flagged as poor quality0
Sequence length248
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGTCCTAGTCGTGATGCAGAGGGGCAGTG89283.5023576578767743No Hit
GTCGTGACTGGGAAAACCCTGGAGCCTAGTCGTGATCCTCGCTAGACAGT84203.303074762468911No Hit
GTCGTGACTGGGAAAACCCTGGATGCTAGTCGTGATAAACATCCGCAGTG81193.1849957240481106No Hit
GTCGTGACTGGGAAAACCCTGGACAGTTGTCGTGATCCTAATGCGCAGTG79243.1084993370313128No Hit
GTCGTGACTGGGAAAACCCTGGGCTCTCGTCGTGATAACAAGGAGCAGTG52952.0771711243792024No Hit
GTCGTGACTGGGAAAACCCTGGACCATTGTCGTGATGCTAAGGGGCAGTG49881.9567383509732694No Hit
GTCGTGACTGGGAAAACCCTGGGGTCTCGTCGTGATCCAAATCCGCAGTG49451.939869916913155No Hit
GTCGTGACTGGGAAAACCCTGGACGGAAGTCGTGATATTGAATGACAGTG47481.8625889515679797No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGCGTCGTGATTATGGCCCGCAGTG44891.760986058043105No Hit
GTCGTGACTGGGAAAACCCTGGTTTCCTGTCGTGATACAGATCCGCAGTG43651.712342201683705No Hit
GTCGTGACTGGGAAAACCCTGGGGGATTGTCGTGATCTAGCTGGCCAGTG43481.705673285892497No Hit
GTCGTGACTGGGAAAACCCTGGTCTAGGGTCGTGATCCAAACACTCAGTG38521.5110978604548984No Hit
GTCGTGACTGGGAAAACCCTGGAGGAGTGTCGTGATTCAGAGTGCCAGTG36451.4298940034678362No Hit
GTCGTGACTGGGAAAACCCTGGTGTTTGGTCGTGATTCGGAGGATCAGTG36031.4134178585719106No Hit
GTCGTGACTGGGAAAACCCTGGTATGTAGTCGTGATAAGGACTCCCAGTG32361.2694477353146552No Hit
GTCGTGACTGGGAAAACCCTGGAATACGGTCGTGATCGACTGGGCCAGTG32161.261601952030881No Hit
GTCGTGACTGGGAAAACCCTGGTGTGCGGTCGTGATCCTTGCACCCAGTG31151.2219807464478216No Hit
GTCGTGACTGGGAAAACCCTGGGTGCCCGTCGTGATGTTGATGGGCAGTG30591.2000125532532542No Hit
GTCGTGACTGGGAAAACCCTGGCGCTCAGTCGTGATATGTTACCTCAGTG28151.1042939971912096No Hit
GTCGTGACTGGGAAAACCCTGGGAACTCGTCGTGATAGTTGTAAACAGTG27971.0972327922358127No Hit
GTCGTGACTGGGAAAACCCTGGGAATAGGCCTGATAGGTGGGTTCCAGTG27751.0886024306236615No Hit
GTCGTGACTGGGAAAACCCTGGTGTGATGTCGTGATCCTAAGCCCCAGTG27711.0870332739669066No Hit
GTCGTGACTGGGAAAACCCTGGAGCCCGGTCGTGATTTAGCGTGCCAGTG26551.0415277309210165No Hit
GTCGTGACTGGGAAAACCCTGGTCATTCGTCGTGATTTATGCGGCCAGTG23540.9234486925002159No Hit
GTCGTGACTGGGAAAACCCTGGCCCGCGGTCGTGATGGGTGGTTGCAGTG22960.9006959209772708No Hit
GTCGTGACTGGGAAAACCCTGGAAACTTGTCGTGATCCAGCCACGCAGTG22670.8893195352157983No Hit
GTCGTGACTGGGAAAACCCTGGCGGTTAGTCGTGATTCATAGGGCCAGTG22160.8693127878421741No Hit
GTCGTGACTGGGAAAACCCTGGGTACAGGTCGTGATGCTACGTATCAGTG20420.8010544732733392No Hit
GTCGTGACTGGGAAAACCCTGGCCTCGTGTCGTGATCCCCCCGAACAGTG20300.7963470033030747No Hit
GTCGTGACTGGGAAAACCCTGGGGCGTGGTCGTGATGCGATCAAACAGTG20230.7936009791537537No Hit
GTCGTGACTGGGAAAACCCTGGGCGACTGTCGTGATATCGGGAGACAGTG19600.7688867618098654No Hit
GTCGTGACTGGGAAAACCCTGGCCCATAGTCGTGATCATAACATTCAGTG17960.7045513388829173No Hit
GTCGTGACTGGGAAAACCCTGGGAATAGGCGTGATAGGTGGGTTCCAGTG17440.6841523023451046No Hit
GTCGTGACTGGGAAAACCCTGGCGGTAGGTCGTGATTGCGACCGCCAGTG15160.5947103729100794No Hit
GTCGTGACTGGGAAAACCCTGGGCACATGTCGTGATAATGACCCGCAGTG14080.5523431431776992No Hit
GTCGTGACTGGGAAAACCCTGGTATCCCGTCGTGATGCTAGTGGGCAGTG13540.531159528311509No Hit
GTCGTGACTGGGAAAACCCTGGAACCGGGTCGTGATCGGGACTCCCAGTG13090.5135065159230172No Hit
GTCGTGACTGGGAAAACCCTGGAGGCAAGTCGTGATTAGAGCTGTCAGTG12820.502914708489922No Hit
GTCGTGACTGGGAAAACCCTGGGGCATAGGATTTTGGCCATGGCTCAGTG9070.35580627191915704No Hit
GTCGTGACTGGGAAAACCCTGGAACCTTGTCGTGATACAACGCCCCAGTG8870.3479604886353829No Hit
GTCGTGACTGGGAAAACCCTGGGTCTCCGTCGTGATCATAATGGGCAGTG8310.3259922954408153No Hit
GTCGTGACTGGGAAAACCCTGGCTACCAGTCGTGATTACAATGGGCAGTG6570.25773398087198035No Hit
GTCGTGACTGGGAAAACCCTGGGCGTGGTCGTGATCATGATAAAGCAGTG6060.2377272334983563No Hit
GTCGTGACTGGGAAAACCCTGGGCGTGGTCCTGATCATGATAAAGCAGTG5730.224781691080129No Hit
GTCGTGACTGGGAAAACCCTGACCGTAGTCCTTATGTCTTATGGCCAGTG5690.22321253442337416No Hit
GTCGTGACTGGGAAAACCCTGGTCGAGAGTCGTGATTCAAGTGACGCAGG5150.20202891955718397No Hit
GTCGTGACTGGGAAAACCCGGGGTCCTAGTCGTGATGCAGAGGGGCAGTG4380.17182265391465357No Hit
GTCGTGACTGGGAAAACCCTGGGGTACTGTCGTGATGTTGATGGGCAGTG3950.1549542198545392No Hit
GTCGTGACTGGGAAAACCCTGGTGAACCGTCGTGATCTTAAACCGCAGTG3710.14553927991401022No Hit
GTCGTGACTGGAAAAACCCTGGAGGCAAGTCGTGATTAGAGCTGTCAGTG3620.14200867743631188No Hit
GTCGTGACTGGGAAAACCCGGGTATGTAGTCGTGATAAGGACTCCCAGTG3140.12317879755525393No Hit
GTCGTGACTGGGAAAACCCTGACCGTAGTCCATATGTCTTATGGCCAGTG3090.12121735173431039No Hit
GTCGTGACTGGGAAAACCCTGGAATATTGTCGTGATATATGTAAACAGTG2800.10984096597283789No Hit
GTCGTGACTGGGAAAACCCTGGCCCACAGTCGTGATAGAAGGTGTGCAGA2740.10748723098770567No Hit
GTCGTGACTGGGAAAACCCTGACCGTAGTCCTGATGTCTTATGGCCAGTG2690.10552578516676211No Hit
GTCGTGACTGGGAAAACCCTGGCCAAGCGTCGTGATCGGAGCGTCCAGTG2670.10474120683838471No Hit
GTCGTGACTGGGAAAACCCTGGCACTCCGTCGTGATTCAAAGGGACAGTG2650.1039566285100073No Hit
GTCGTGACTGGGAAAACCCTGGTCTTACGTCGTGATAACTATGAAACAGT2620.10277976101744118No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTG100.001520082242.577591
ACGTGAC450.0242.386922
CGTGAGG100.0015254591242.29173
CGAGACT251.720764E-9242.29173
TCGTGAC232300.0242.23042
AGACTGG251.7280399E-9242.1495
TAGGAAA301.8189894E-11242.148999
ACTGGGA231600.0241.521687
CGTGACT232100.0239.73413
GTGACTG235450.0239.414754
TGACTGG234550.0239.30995
TGGGAAA228600.0238.65349
GACTGGG238400.0235.292836
CTGGGAA240900.0231.091988
GGTGACT3200.0230.934283
GTGACGG1900.0229.494424
GGTGACG800.0227.148473
TGACAGG2700.0224.212055
ACGGGGA1950.0223.522167
TCGTGAG550.0220.351752
Produced by FastQC (version 0.11.5)