FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284409
Sequences flagged as poor quality0
Sequence length248
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCACAATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT29700.23123475466148244Illumina Single End PCR Primer 1 (97% over 38bp)
GACCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT26230.20421843820776714Illumina Single End PCR Primer 1 (97% over 38bp)
GACCACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT16620.12939803442672856Illumina Single End PCR Primer 1 (97% over 38bp)
GACCACAATATCGGAACAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT15250.11873165012079485Illumina Single End PCR Primer 1 (96% over 28bp)
GACCACGATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT14040.1093109749308826Illumina Single End PCR Primer 1 (97% over 38bp)
GACCACAATATCGGAACACCGTCGTGTAGGCAAAGAGTGTAGATCCAGAT13130.10222600433351058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)