FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167563
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTTCAATGTCGTGATCAATACGGACGATG3484720.796357191026658No Hit
GTCGTGACTGGGAAAACCCTGGGAGCGGGTCGTGATGTTAAATGCCGATG144428.618847836336183No Hit
GTCGTGACTGGGAAAACCCTGGGACGCAGTCGTGATCATAAGAACCGATG86005.132397963750948No Hit
GTCGTGACTGGGAAAACCCTGGTGTAACGTCGTGATGCCATGAAACGATG77594.630497186132978No Hit
GTCGTGACTGGGAAAACCCTGGTTAGTTGTCGTGATCTAGTCAGCCGATG73314.375070868867232No Hit
GTCGTGACTGGGAAAACCCTGGCATGTTGTCGTGATGAGCAGTGGCGATG56573.3760436373185008No Hit
GTCGTGACTGGGAAAACCCTGGAGCTAAGTCGTGATTTCCGGACCCGATG53903.216700584257861No Hit
GTCGTGACTGGGAAAACCCTGGTACGCGGTCGTGATATCCAGAAACGATG41322.46593818444406No Hit
GTCGTGACTGGGAAAACCCTGGCATTTAGTCGTGATTGATTCTACCGATG34082.0338618907515382No Hit
GTCGTGACTGGGAAAACCCGGGTTCAATGTCGTGATCAATACGGACGATG33221.9825379111140289No Hit
GTCGTGACTGGGAAAACCCTGGATTGCGGTCGTGATCATCAGAACCGATG28081.6757876142107744No Hit
GTCGTGACGGGAAAACCCTGGCATGTTGTCGTGATGAGCAGTGGCCGATG25391.515250980228332No Hit
GTCGTGACGGGAAAACCCTGGCATGTTGTCCTGATGAGCAGTGGCCGATG24741.4764596002697492No Hit
GTCGTGACTGGGAAAACCCTGGTCCCACGTCGTGATAGATGCAGTCGATG21021.254453548814476No Hit
GTCGTGACTGGGAAAACCCTGGACCAAAGTCGTGATTAGAGCACGCGATG15800.9429289282240112No Hit
GTCGTGACTGGGAAAACCCTGGCCACCTGTCGTGATCAACCGGTCCGATG13140.7841826656242726No Hit
GTCGTGACTGGGAAAACCCTGGCTCATTGTCGTGATATGCGGAACCGATG11440.6827282872710563No Hit
GTCGTGACTGGTAAAACCCTGGGAGCGGGTCGTGATGTTAAATGCCGATG11410.6809379158883524No Hit
GTGGTGACTGGGAAAACCCTGGTTCAATGTCGTGATCAATACGGACGATG8730.5209980723668113No Hit
GTCGTGACTGGAAAACCCTGGCATGTTGTCCTGATGAGCAGTGGCCGATG7610.4541575407458687No Hit
GTCGTGACTGGGAAAACCCGGGGAGCGGGTCGTGATGTTAAATGCCGATG6690.39925281834295157No Hit
GTCGTGACGGGAAAACCCTTGCATGTTGTCCTGATGAGCAGTGGCCGATG6640.39626886603844524No Hit
GTCGTGACTGGGAAAACCCTGGTACGCGGTCGTGATATCCAGAACCGATG6130.3658325525324803No Hit
GTGGTGACTGGGAAAACCCGGGTTCAATGTCGTGATCAATACGGACGATG5590.33360586764381156No Hit
GTCGTGACGGGAAAACCCTTGCATGTTGTCGTGATGAGCAGTGGCCGATG5220.3115246205904645No Hit
GTCGTGACTGGGAAAACCCTGGTTCAAAGTCGTGATCAATACGGACGATG5080.30316955413784663No Hit
GTCGTGACTGGGAAAACCCTGGCTCATTGTCGTGATATCCAGAACCGATG4970.29660485906793266No Hit
GTCGTGACTGGGAAAACCCGGGTTAGTTGTCGTGATCTAGTCAGCCGATG4860.29004016399801863No Hit
GTCGTGACTGGAAAACCCTGGCATGTTGTCGTGATGAGCAGTGGCCGATG4830.28824979261531486No Hit
GTCGTGACAGGGAAAACCCTGGTTCAATGTCGTGATCAATACGGACGATG4400.26258780279656013No Hit
GTCGTGACTGGGAACACCCTGGTTCAATGTCGTGATCAATACGGACGATG4370.26079743141385625No Hit
GTCGTGACTGGGAAACCCCTGGTTCAATGTCGTGATCAATACGGACGATG4320.25781347910934993No Hit
GTCGTGACTGGGAAAACCCTGGATCAATGTCGTGATCAATACGGACGATG3970.23692581297780538No Hit
GTCGTGACTGGGAAAACCCTGGCTCATTGTCGTGATATGCAGAACCGATG3900.23274827975149648No Hit
GTCGTGACTGGGAAAACCCTGGTTCAATGTCGTGATCAAAACGGACGATG3760.22439321329887862No Hit
GTCGTGACGGGAAAACCCTGGCATGTTGTCCTGATTAGCAGTGGCCGATG3450.20589270901093917No Hit
GTCGTGACTGGGAAAACGCTGGTTCAATGTCGTGATCAATACGGACGATG3250.19395689979291372No Hit
GTCGTGACTGGGAAAACCCTGGCTCATTGTCGTGATATCCGGAACCGATG3220.1921665284102099No Hit
GTCGTGACTGGAAAACCCTTGCATGTTGTCCTGATGAGCAGTGGCCGATG2730.16292379582604752No Hit
GTCGTGACTGGGAAAACCCTGGCCACCTGTCGTGATCAAACGGTCCGATG2620.15635910075613352No Hit
GTCGTGACTGGGAAAACCCTGGTACAATGTCGTGATCAATACGGACGATG2180.13010032047647752No Hit
GTCGTGACTGGGAAAACCCGGGCATTTAGTCGTGATTGATTCTACCGATG2170.12950353001557624No Hit
GTCGTGACTGGGAAAACCCTGGGGACTGGTCGTGATCAGTAGGGTCGATG1930.11518055895394569No Hit
GTGGTGACTGGGAAAACCCTGGGAGCGGGTCGTGATGTTAAATGCCGATG1930.11518055895394569No Hit
GTCGGGACTGGGAAAACCCTGGTTCAATGTCGTGATCAATACGGACGATG1810.1080190734231304No Hit
GTCGTGACTGGGAATACCCTGGTTCAATGTCGTGATCAATACGGACGATG1780.10622870204042659No Hit
GTCGTGACTGGGAAAACCCTGGGAGCGGGTCGTGATGTTAAAAGCCGATG1730.10324474973592021No Hit
GTCGTGACAGGGAAAACCCTGGGAGCGGGTCGTGATGTTAAATGCCGATG1700.1014543783532164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGAC100.0015214842242.478162
CGAGACT251.720764E-9242.260853
TGTGACT100.0015255755242.26083
TCGTGAC157600.0242.247392
CGTGACT146700.0242.178273
AGTGGGA650.0242.116157
ACTGGGA147900.0242.116157
GGGGAAC201.6525519E-7242.116139
CACTGGG100.0015283071242.116136
CGGGAAC100.0015283071242.116138
TCACTGG100.0015283071242.116135
GACTCGG100.0015283071242.116136
GTGACTG150100.0242.035494
TGACTGG150050.0242.035485
TGGTGAC3950.0239.408812
GACTGGG150400.0238.816026
CGTGACG10250.0238.715563
GGTGACT3350.0238.6453
ACGGGAA9300.0238.211077
CGGGAAA9200.0238.16868
Produced by FastQC (version 0.11.5)