FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441911
Sequences flagged as poor quality0
Sequence length248
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGCTAGGGTCGTGATATCTGGAGACAGAT42310.957432605207836No Hit
GTCGTGACTGGGAAAACCCTGGCGTAAGGTCGTGATCTGCTCTGTCAGAT41870.9474758492094563No Hit
GTCGTGACTGGGAAAACCCTGGGCTCTCGTCGTGATGAATAGTACCAGGG10790.24416681186935832No Hit
GACCACAAGATCGGAACTCCGTCGTGTAGGCAAAGAGTGTAGATCCAGAT10470.23692553477962755No Hit
GTCGTGACTGGGAAAACCCTGGGTAGTCGTCGTGATACGGCTTCGCAGAT8850.20026656951286573No Hit
GACCACAAGATCGGAAGTGCGTCGTGTAGGCAAAGAGTGTAGATCCAGAT8280.18736804469678284Illumina Single End PCR Primer 1 (95% over 23bp)
GACCACATGATCGGAAGTGCGTCGTGTAGGCAAAGAGTGTAGATCCAGAT7040.15930809597407622Illumina Single End PCR Primer 1 (96% over 27bp)
GTCGTGACTGGGAAAACCCTGGCTGTACGTCGTGATCTTGATCCGCAGAT6890.15591374733826494No Hit
GTCGTGACTGGGAAAACCCTGGAGTTTAGTCGTGATCGCAAGTTTCATGA6500.1470884408851556No Hit
GACCACATGATCGGAACTCCGTCGTGTAGGCAAAGAGTGTAGATCCAGAT6250.14143119315880348Illumina Single End PCR Primer 1 (96% over 26bp)
GAGCACATGATCGGAAGTGCGTCGTGTAGGCAAAGAGTGTAGATCCAGAT5550.12559089952501748Illumina Single End PCR Primer 1 (96% over 27bp)
GTCGTGACTGGGAAAACCCTGGTACCAGGTCGTGATGGGTTCTGACAGAT5490.12423316007069296No Hit
GACCACAAGATCGGAACACCGTCGTGTAGGCAAAGAGTGTAGATCCAGAT5460.12355429034353071Illumina Single End PCR Primer 1 (96% over 26bp)
GTCGTGACTGGGAAAACCCGGGCGTAAGGTCGTGATCTGCTCTGTCAGAT5000.11314495452704279No Hit
GTCGTGACTGGGAAAACCCTGGAAGCTAGTCGTGATTTGGATAGCCAGAT4680.10590367743731202No Hit
GTCGTGACTGGGAAAACCCTGGTATATTGTCGTGATAAAAAGTCCCAGAT4420.10002013980190581No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACG550.0242.4751
GATCGCT151.5828311E-5242.474991
GGCCGTA251.715307E-9242.474991
GGCCGAT100.0015223862242.474991
GTTAGCA201.6434024E-7242.474991
GCGCAAA251.715307E-9242.474991
GTTAACT151.5828311E-5242.474991
GTGCTAA100.0015223862242.474991
GTTCGCA151.5828311E-5242.474991
GTCCGTT201.6434024E-7242.474991
GTCCGAT201.6434024E-7242.474991
GAGTTCT100.0015223862242.474991
GGACATT100.0015223862242.474991
GCGCTCA100.0015223862242.474991
GTACGAT100.0015223862242.474991
TTCTATT100.001524972242.33762
TTAGGTT100.001524972242.33762
GCCTTGT201.648059E-7242.33762
TCCGCTA100.001524972242.33762
TTAGCGA100.001524972242.33762
Produced by FastQC (version 0.11.5)