FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149704
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTAATAGGTCGTGATAGAGTGGAAACCTA15951.0654357932987764No Hit
GTCGTGACTGGGAAAACCCTGGGAATCGGTCGTGATGGCTACCCCGCCAA11470.7661785924223802No Hit
GTCGTGACTGGGAAAACCCTGGAGGTACGTCGTGATGCAGCTTCGGCCAA7870.5257040560038476No Hit
GTCGTGACTGGGAAAACCCTGGCGGAGTGTCGTGATACCGTTATTGCCAA6960.4649174370758296No Hit
GTCGTGACTGGGAAAACCCTGGAACACTGTCGTGATTTAATCCATGCCAA5710.38141933415272805No Hit
GTCGTGACTGGGAAAACCCTGGCTATCTGTCGTGATGTACGTGGGGCCAA5340.35670389568749No Hit
GTCGTGACTGGGAAAACCCTGGTACAAGGTCGTGATAGAAACCCGGCCAA4910.32798054828194306No Hit
GTCGTGACTGGGAAAACCCTGGATGACTGTCGTGATTAGAGTGTGTCCAT4480.29925720087639607No Hit
GTCGTGACTGGGAAAACCCTGGCCGTTCGGTCGTGATATAAACCCGCCAA2790.18636776572436273No Hit
GTCGTGACTGGGAAAACCCTGGCTTATAGTCGTGATTGCGAAGGAGCCAA1880.12558114679634477No Hit
GTCGTGACTGGGAAAACCCTGGTGAGCCGTCGTGATCAAAATTCCGCCAA1750.11689734409234222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT130100.0242.048363
TCGTGAC136200.0241.960022
GTGACTG131850.0240.32964
TGACTGG131400.0239.96625
GACTGGG133050.0237.536356
ACTGGGA133650.0233.842537
TGGGAAA131600.0232.33259
CGTGACC3150.0227.02923
CGTGACA1550.0226.780963
GTGACCT3000.0226.108284
CTGGGAA137100.0225.131798
GTGACGA1450.0208.843864
TGACGAC1450.0208.774025
CGTGACG2450.0207.789443
TGACGGG1650.0198.145545
TCGTGGC1600.0189.518282
CGTGGCT1900.0185.005513
TGACCTG4000.0175.578955
GTGACGG2050.0171.353824
TGACAGG2300.0163.206825
Produced by FastQC (version 0.11.5)