FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4479711
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCTACCCGTCGTGATCTCATATACTGCAC89010.19869585337089823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC4422950.0241.195632
GTGACTG4424550.0240.868644
TGACTGG4426900.0240.758625
ACTGGGA4420400.0240.71337
TGGGAAA4433500.0239.982999
GACTGGG4450700.0239.240286
CGTGACT4542600.0234.703983
CTGGGAA4582200.0232.137048
ACGTGAC6750.0147.189542
CGAGACT11300.0137.15213
GACTGGA16800.0132.522176
TCGAGAC11150.0127.138752
GTCGTGA8623950.0123.6967541
CTGCGAA5600.0110.191378
GTGACCG11800.0105.659784
ACCGGGA12450.0100.100947
ACTCGGA3100.097.5772557
CGGACTG3700.094.9002153
GACGTGA9800.092.754121
TCGGACT4450.089.850572
Produced by FastQC (version 0.11.5)