FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748406
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTCCTTCGTCGTGATGGGGCGTAGTAGCT152902.043008741244725No Hit
GTCGTGACTGGGAAAACCCTGGGTGGTTGTCGTGATACGGTGTTCTAGCT127291.7008147983848336No Hit
GTCGTGACTGGGAAAACCCTGGCGGGGTGTCGTGATGGTATACCCTAGCT116831.5610510872440895No Hit
GTCGTGACTGGGAAAACCCTGGGGAATCGTCGTGACGAGACCCCATAGCT115581.5443489229108265No Hit
GTCGTGACTGGGAAAACCCTGGAACGACGTCGTGATGGGAACGCATAGCT113761.5200305716415956No Hit
GTCGTGACTGGGAAAACCCTGGGGATCGGTCGTGATGAGTGCTCGTAGCT112461.5026603207350022No Hit
GTCGTGACTGGGAAAACCCTGGCGGCCTGTCGTGATGCTAGGGTGTAGCT111641.4917037009323817No Hit
GTCGTGACTGGGAAAACCCTGGGTGCAAGTCGTGATACGTATAAATAGCT106741.4262312167459907No Hit
GTCGTGACTGGGAAAACCCTGGAAGTGAGTCGTGATAGGACGTTCTAGCT105921.4152745969433702No Hit
GTCGTGACTGGGAAAACCCTGGTACTCCGTCGTGATAGCAGGGTCTAGCT105161.4051196810287463No Hit
GTCGTGACTGGGAAAACCCTGGGTTATTGTCGTGATAAAATACCCTAGCT96561.2902087904158972No Hit
GTCGTGACTGGGAAAACCCTGGATCTATGTCGTGATCGGTCCCCCTAGCT94881.2677610815519917No Hit
GTCGTGACTGGGAAAACCCTGGGACAGGGTCGTGATCAAGTGTTCTAGCT91351.2205941694748572No Hit
GTCGTGACTGGGAAAACCCTGGCCTGCCGTCGTGATACCAAAGACTAGCT91181.2183226751255334No Hit
GTCGTGACTGGGAAAACCCTGGGAAGAAGTCGTGATTGGAAACCCTAGCT90241.2057626475469196No Hit
GTCGTGACTGGGAAAACCCTGGCTCCTCGTCGTGATGGTTTGTTCTAGCT88841.1870562234936652No Hit
GTCGTGACTGGGAAAACCCTGGCATCATGTCGTGATAACGTGCCCTAGCT88481.1822460001656856No Hit
GTCGTGACTGGGAAAACCCTGGGTGCTAGTCGTGATACTTTGTTCTAGCT85471.1420271884511883No Hit
GTCGTGACTGGGAAAACCCTGGGTGGAGGTCGTGATTGACTGTTCTAGCT84191.124924172173927No Hit
GTCGTGACTGGGAAAACCCTGGCCACGGGTCGTGATTCACGACGTTAGCT83011.1091573290433268No Hit
GTCGTGACTGGGAAAACCCTGGGGGTGCGTCGTGATGCAGGACTATAGCT81811.0931232512833944No Hit
GTCGTGACTGGGAAAACCCTGGTGCCGAGTCGTGATCAACCACCCTAGCT80621.077222790838128No Hit
GTCGTGACTGGGAAAACCCTGGGCCTTCGTCGTGATAAAAGCCCATAGCT79461.0617231823368598No Hit
GTCGTGACTGGGAAAACCCTGGGTGTTCGTCGTGATGGTATAACTTAGCT77881.0406116466196156No Hit
GTCGTGACTGGGAAAACCCTGGACCGCAGTCGTGATAATACACCCTAGCT76861.0269826805236728No Hit
GTCGTGACTGGGAAAACCCTGGCCGCATGTCGTGATCGACGTCTTTAGCT75221.005069440918432No Hit
GTCGTGACTGGGAAAACCCTGGCCACAGGTCGTGATGAATGCTAGTAGCT74931.001194538793115No Hit
GTCGTGACTGGGAAAACCCTGGCGCGCGGTCGTGATATAGTGCCCTAGCT74520.9957162288918048No Hit
GTCGTGACTGGGAAAACCCTGGGTCATCGTCGTGATAGCATCACTTAGCT72970.9750055451185586No Hit
GTCGTGACTGGGAAAACCCTGGGGGGCCGTCGTGATGGCGCGGTTTAGCT72120.9636480733719399No Hit
GTCGTGACTGGGAAAACCCTGGAACCTCGTCGTGATTTGGAGTTCTAGCT71480.9550965652333092No Hit
GTCGTGACTGGGAAAACCCTGGCTCTTAGTCGTGATCGCAGTGGCTAGCT69830.9330497083134021No Hit
GTCGTGACTGGGAAAACCCTGGAAGCTAGTCGTGATTATAGCCCATAGCT68980.9216922365667833No Hit
GTCGTGACTGGGAAAACCCTGGTCAGACGTCGTGATTTAAACCAATAGCT67940.9077960358415085No Hit
GTCGTGACTGGGAAAACCCTGGACCGCTGTCGTGATGTGTGCTTCTAGCT64880.8669091375536807No Hit
GTCGTGACTGGGAAAACCCTGGACCGATGTCGTGATAGATTCTTATAGCT58930.787406835327349No Hit
GTCGTGACTGGGAAAACCCTGGTCAGGCGTCGTGATAGCCCGTGGTAGCT54010.721667116511626No Hit
GTCGTGACTGGGAAAACCCTGGCATCCAGTCGTGATCCACATGGTTAGCT52900.7068355945836885No Hit
GTCGTGACTGGGAAAACCCTGGTCAATAGTCGTGATCAAGATTCTTAGCT52140.6966806786690647No Hit
GTCGTGACTGGGAAAACCCTGGCCACACGTCGTGATATCAATCCCTAGCT51970.6944091843197409No Hit
GTCGTGACTGGGAAAACCCTGGGCTCGAGTCGTGATGCCTTTTTATAGCT49310.6588669786185574No Hit
GTCGTGACTGGGAAAACCCTGGAAGAGTGTCGTGATTGCTAAAAATAGCT48100.6426992835439588No Hit
GTCGTGACTGGGAAAACCCTGGCATATGGTCGTGATCAGCAGTTCTAGCT47520.6349494792933248No Hit
GTCGTGACTGGGAAAACCCTGGCTCTCAGTCGTGATTTATGCCCCTAGCT46680.623725624861372No Hit
GTCGTGACTGGGAAAACCCTGGTTGCCAGTCGTGATGTGGTCTAGTAGCT46440.6205188093093855No Hit
GTCGTGACTGGGAAAACCCTGGCCCCCAATAAAGCTAAAACTCACTAGCT45710.61076474533876No Hit
GTCGTGACTGGGAAAACCCTGGATAGGAGTCGTGATTAAGCCCCGTAGCT45130.603014941088126No Hit
GTCGTGACTGGGAAAACCCTGGTGAAAGGTCGTGATTCATTCCCCTAGCT45120.6028813237734598No Hit
GTCGTGACTGGGAAAACCCTGGACTCGTGTCGTGATGTAGTGTTCTAGCT45090.6024804718294615No Hit
GTCGTGACTGGGAAAACCCTGGGGACATGTCGTGATTCAAACTACTAGCT44400.5932608771175004No Hit
GTCGTGACTGGGAAAACCCTGGACCCACGTCGTGATACAGAGAGTTAGCT42700.5705459336242628No Hit
GTCGTGACTGGGAAAACCCTGGATAACCGTCGTGATAAAGATGGATAGCT40700.5438224706910421No Hit
GTCGTGACTGGGAAAACCCTGGTACCGAGTCGTGATGAGAAACCCTAGCT40010.5346028759790808No Hit
GTCGTGACTGGGAAAACCCTGGATTATGGTCGTGATTATGCGATCTAGCT39150.5231117869177959No Hit
GTCGTGACTGGGAAAACCCTGGGTGGGGGTCGTGATTGAATTACCTAGCT36130.48275935788863267No Hit
GTCGTGACTGGGAAAACCCTGGAATGTGGTCGTGATATATACCCTTAGCT36120.48262574057396657No Hit
GTCGTGACTGGGAAAACCCTGGAGACCCGTCGTGATATGGTGACATAGCT35320.47193635540067824No Hit
GTCGTGACTGGGAAAACCCTGGGCAGGTGTCGTGATACTAACTGGTAGCT35010.467794218646029No Hit
GTCGTGACTGGGAAAACCCTGGTAAGCTGTCGTGATAGTGATTAGTAGCT33770.4512256716274322No Hit
GTCGTGACTGGGAAAACCCTGGCTCGTTGTCGTGATGTCAACGAGTAGCT33150.44294139811813377No Hit
GTCGTGACTGGGAAAACCCTGGCTTCGAGTCGTGATACAGGACCTTAGCT33100.4422733115448032No Hit
GTCGTGACTGGGAAAACCCTGGGAAGACGTCGTGATTCCGATGGGTAGCT32220.4305149878541861No Hit
GTCGTGACTGGGAAAACCCTGGCCTGACGTCGTGATGTGAGAACCTAGCT31570.4218298624008894No Hit
GTCGTGACTGGGAAAACCCTGGATCTCTGTCGTGATGTTTACCCCTAGCT30820.4118085638009316No Hit
GTCGTGACTGGGAAAACCCTGGTTATATGTCGTGATAAGATTGGGTAGCT30650.40953706945160784No Hit
GTCGTGACTGGGAAAACCCTGGCGATCGGTCGTGATTCTGAGTTGTAGCT30590.4087353655636112No Hit
GTCGTGACTGGGAAAACCCTGGTACCACGTCGTGATAGCAATGGGTAGCT28970.38708936058770244No Hit
GTCGTGACTGGGAAAACCCTGGAGTTTCGTCGTGATCCCTACGGGTAGCT28470.3804084948543972No Hit
GTCGTGACTGGGAAAACCCTGGAGCCTCGTCGTGATTTACAGTACTAGCT28440.3800076429103989No Hit
GTCGTGACTGGGAAAACCCTGGAACATTGTCGTGATCAATACAGCTAGCT27820.37172336940110046No Hit
GTCGTGACTGGGAAAACCCTGGGAAATAGTCGTATAATAAACCAATAGCT27040.3613012188571444No Hit
GTCGTGACTGGGAAAACCCTGGTCCTGAGTCGTGATCCTGCTCAATAGCT26840.3586288725638223No Hit
GTCGTGACTGGGAAAACCCTGGACAAATGTCGTGATCGGTAATTATAGCT25820.34499990646787976No Hit
GTCGTGACTGGGAAAACCCTGGTTTCCCGTAACGGGGACGGCCGGTAGCT23760.3174747396466624No Hit
GTCGTGACTGGGAAAACCCTGGGGTGCCGTCGTGATGGCGTTGCCTAGCT23430.31306536826268094No Hit
GTCGTGACTGGGAAAACCCTGGCTACCGGTCGTGATAAGATATACTAGCT22610.3021087484600604No Hit
GTCGTGACTGGGAAAACCTGGTATGTAGTCCTGATCTAACTCTGATAGCT22130.29569511735608744No Hit
GTCGTGACTGGGAAAACCCTGGCCAATGGGTCGTGATGATCCGCCTAGCT21200.28326870709213986No Hit
GTCGTGACTGGGAAAACCCTGGAATAGTGTCGTGATTTGCATTCTTAGCT20130.26897165442286675No Hit
GTCGTGACTGGGAAAACCCTGGATTCTCGTCGTGATTCCGACCTTTAGCT20090.2684371851642023No Hit
GTCGTGACTGGGAAAACCTGGTATGTAGTCGTGATCTAACTCTGATAGCT18980.2536056632362648No Hit
GTCGTGACTGGGAAAACCCTGGACTGTCGTCGTGATATGAAGTGGTAGCT17820.23810605473499677No Hit
GTCGTGACTGGGAAAACCCTGGGACCTGGTCGTGATCTGTGAACATAGCT17060.22795113882037288No Hit
GTCGTGACTGGGAAAACCCTGGCCTATGGTCGTGATGGTAAGTGCTAGCT16290.2176626055910829No Hit
GTCGTGACTGGGAAAACCCTGGTTGTGCGTCGTGATCCTGCGTTCTAGCT15590.20830939356445566No Hit
GTCGTGACTGGGAAAACCCTGGACCATCGTCGTGATCCAAACTCCTAGCT14530.19414595820984865No Hit
GTCGTGACTGGGAAAACCCTGGAACCTCGTCGTGATTTGGAGTTATAGCT14400.19240893311918933No Hit
GTCGTGACTGGGAAAACCCTGGCAACCGTCCTGATCTACATAAACTAGCT13960.18652977127388076No Hit
GTCGTGACTGGGAAAACCCTGGGTAGAGTCCTGATACAATGAGGGTAGCT13640.18225401720456544No Hit
GTCGTGACTGGGAAAACCCTGGCACAGAGTCGTGATAAAATGGATTAGCT11670.155931406215343No Hit
GTCGTGACTGGGAAAACCCTGGCTGCGAGTCGTGATCGGAGGGACTATCT11380.152056504090026No Hit
GTCGTGACTGGGAAAACCCTGGACTGGGGTCGTGATCCCGAGGGGTAGCT11350.1516556521460277No Hit
GTCGTGACTGGGAAAACCCTGGCTACAGGTCGTGATAGATGTTAGTAGCT11270.15058671362869885No Hit
GTCGTGACTGGGAAAACCCTGGGTAGAGTCGTGATACAATGAGGGTAGCT10260.13709136484742238No Hit
GTCGTGACTGGGAAAACCCTGGCCTTACGTCGTGATTCAAGACTTTAGCT9950.13294922809277315No Hit
GTCGTGACTGGGAAAACCCTGGATGAACGTCGTATTGGCTGTCTGTAGCT9830.1313458203167799No Hit
GTCGTGACTGGGAAAACCCTGGTGTACTGTCGTGATAATTATTACTAGCT9380.12533304115680527No Hit
GTCGTGACTGGGAAAACCCTGGTGGTATGTCGTGATAGCGATGGGTAGCT9220.12319516412214761No Hit
GTCGTGACTGGGAAAACCCTGGGTGCTCGTCGTGATGGTTTGTTCTAGCT9160.12239346023415099No Hit
GTCGTGACTGGGAAAACCCTGGCAACCGTCGTGATCTACATAAACTAGCT8440.11277301357819151No Hit
GTCGTGACTGGGAAAACCCTGGCATCTTGTCGTGATTTACGGTCATAGCT7960.10635938247421854No Hit
GTCGTGACTGGGAAAACCCTGGTTTCCCGTAACGGTGACGGCCGGTAGCT7730.10328618423689816No Hit
GTCGTGACTGGGAAAACCCTGGTCCTGAGTCGAGATAAAGCTCAATAGCT7500.10021298599957777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)