FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842880
Sequences flagged as poor quality0
Sequence length248
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGACCCTGCCGTCGTGATGCCGCGAGATCAC19480.23111237661351558No Hit
GTCGTGACTGGGAAAACCCTGGCCCTATGTCGTGAATGTAACCCAACCTC13460.15969058466211086No Hit
GTCGTGACTGGGAAAACCCTGGTGGGCCGTCGTGATTGTTACGAGATCAC12930.15340261958997722No Hit
GTCGTGACTGGGAAAACCCTGGCCTGGGGTCGTGATAGGTACGGGATCAC12420.14735193621867881No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTCGTGATAAAAAAAGATCAC8740.1036921032649962No Hit
GTCGTGACTGGGAAAACCCTGGCCTGCCGTCGTGATGCCGCGAGAATCAC8610.1021497722095672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGGC7300.0242.543622
TCGTGAC803700.0242.317292
CGTGACT793850.0242.097663
GTGACTG796650.0241.937884
TGACTGG796550.0241.746145
GACTGGG797150.0241.49086
ACTGGGA791250.0241.457727
CGTGGCT8300.0240.881973
TGGGAAA788550.0240.350629
CTGGGAA797850.0238.808048
GTGACAG10450.0238.74984
CGTGACA10500.0233.109793
TGACAGG10850.0228.736635
CGAGACT1250.0222.954513
TCGAGAC1150.0221.452882
ACAGGGA11350.0220.727877
GGTGACT3350.0220.639623
AGACTGG1050.0219.066455
GACAGGG11550.0216.93156
TGACCGG1400.0216.183995
Produced by FastQC (version 0.11.5)