FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46225
Sequences flagged as poor quality0
Sequence length248
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCCATTGCCTCTCTCAACTGCACTTACACGATG35077.586803677663602No Hit
GTCGTGACTGGGAAAACCCTGGGCACATGTCGTGATAGGTACTGGCGAAT17603.807463493780422No Hit
GTCGTGACTGGGAAAACCCTGGGCTAACGTCTGATCCTAACTATTCGATG3210.6944294213088156No Hit
GTCGTGACTGGGAAAACCCTGGATATTAGTCGTGATTCAAAGAACCGAAT2660.5754461871281774No Hit
GTCGTGACTGGGAAAACCCTGGCTCATGGTCGTGATGAACTCGGACAATG1580.34180638182801515No Hit
GTCGTGACTGGGAACACCCCATTGCCTCTCTCAACTGCACTTACACGATG730.15792320173066524No Hit
GTCGTGACTGGGAAAACCCTGGGAGCAGGTCGTGATGGAGACCTCCGATG610.13196322336398053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGA201.6436388E-7242.000021
GTGAGTG100.0015269283242.000024
ACGGGGA500.0241.999987
TGACAGG500.0241.999985
ACTGGGA40500.0241.402477
TCGTGAC40500.0241.10372
GTGACTG40800.0239.924034
TGACTGG40750.0239.624545
TGGGAAA39500.0239.549369
GACTGGG41500.0238.209646
CGTGACT40500.0237.219763
CTGGGAA41700.0233.004798
TGGTGAC850.0227.764712
CGTGGCT700.0224.71433
GACGGGG600.0221.833336
CGTGACG400.0211.750023
CGTGACA650.0204.769233
CGGGGAA600.0201.666678
GTGGTGA1050.0195.904751
GTGACGG500.0193.599994
Produced by FastQC (version 0.11.5)