FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339861
Sequences flagged as poor quality0
Sequence length248
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGGTTGGGTCGTGATGTGGATTTCCAGAT170285.0102836159488735No Hit
GTCGTGACTGGGAAAACCCTGGTGACGGTCTGATGTGGAGATAGTCAGAC83002.4421748891458566No Hit
GTCGTGACTGGGAAAACCCTGGTTGCGAGTCGTGATGACCACACGCAGAT46621.371737269059998No Hit
GTCGTGACTGGGAAAACCCGGGGGTTGGGTCGTGATGTGGATTTCCAGAT9010.26510838254462854No Hit
GACCACAAGATCGGAAGTGCGTCGTGTAGGCAAAGAGTGTAGATCCAGAT5140.15123830036397232Illumina Single End PCR Primer 1 (95% over 23bp)
GACCACAAGATCGGAACTCCGTCGTGTAGGCAAAGAGTGTAGATCCAGAT4410.12975893085702683No Hit
GACCACATGATCGGAAGTGCGTCGTGTAGGCAAAGAGTGTAGATCCAGAT4340.12769926528786768Illumina Single End PCR Primer 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATG151.5865631E-5242.319633
CTACGTG100.0015251561242.319613
CGCATTA251.722583E-9242.28394
TCGTGAC206400.0241.75772
GTGACTG203700.0241.629734
ACTGGGA202550.0241.54357
TGACTGG203350.0241.533485
CGTGACT203100.0240.649293
GACTGGG205650.0240.116066
TGGGAAA201050.0239.612019
ATGATCG17950.0238.768777
TATCGGA9700.0237.148629
CTGGGAA205350.0236.741178
TTGATCG8550.0236.47717
CACTAGA1650.0234.941994
TTATCGG5050.0232.517218
ATATCGG5000.0232.421348
AAGATCG26850.0232.22117
TAGATCG11750.0231.837337
TGATCGG40400.0228.921598
Produced by FastQC (version 0.11.5)