FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330041
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGATG17150.1289433934743365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA1303950.0240.998237
TCGTGAC1308400.0240.71772
GTGACTG1310200.0240.354774
TGACTGG1311250.0240.144845
TGGGAAA1311450.0239.66619
GACTGGG1320850.0237.99656
CGTGACT1343000.0234.34823
CTGGGAA1358700.0231.29598
ACCGGGA3350.0162.53767
GACTGGA4550.0159.57876
TCGAGAC3600.0127.8810962
ACGTGAC3150.0126.919592
GTCGTGA2536100.0124.154771
TCGGACT1400.0121.150522
CGAGACT3850.0119.496163
CGGACTG1450.0116.8937153
CGGGGAA3050.0115.049399
TGCGAAA1900.0114.631789
CTGCGAA2050.0106.244098
GACGTGA3850.0103.8628541
Produced by FastQC (version 0.11.5)