FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973361
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGAGGTAGTCGTGATCCTTTGAAGTAGCT56200.1890116941736977No Hit
GTCGTGACTGGGAAAACCCTGGGTGCGGGTCGTGATATTAAGTGATAGCT46020.15477434458849765No Hit
GTCGTGACTGGGAAAACCCTGGCTAACAGTCGTGATGCTCGTTGATAGCT41890.14088433930491454No Hit
GTCGTGACTGGGAAAACCCTGGTCCTCAGTCGTGATGGACACTCCTCGCT39510.1328799294804768No Hit
GTCGTGACTGGGAAAACCCTGGCGCCGTGTCGTGATTTAGAACCCTAGCT37740.1269270700732269No Hit
GTCGTGACTGGGAAAACCCTGGGCCAGCGTCGTGATAGGACCCCTTAGCT37660.12665801428080883No Hit
GTCGTGACTGGGAAAACCCTGGACGGTGGTCGTGATGAGATTATGTAGCT37230.12521183939656166No Hit
GTCGTGACTGGGAAAACCCTGGCTTAAAGTCGTGATCCTAAGGAGTAGCT36170.12164685014702217No Hit
GTCGTGACTGGGAAAACCCTGGCCAGGCGTCGTGATCAAAGCAGCTAGCT36140.12154595422486539No Hit
GTCGTGACTGGGAAAACCCTGGCCACGGGTCGTGATCAGATCATGTAGCT36110.12144505830270862No Hit
GTCGTGACTGGGAAAACCCTGGGACAGTGTCGTGATACGGCGGTCTAGCT35640.11986435552225243No Hit
GTCGTGACTGGGAAAACCCTGGGTCGTAGTCGTGATATGCTCATCTAGCT35180.1183172847158485No Hit
GTCGTGACTGGGAAAACCCTGGTTCTGCGTCGTGATGCATAGGAATAGCT35010.11774554115696009No Hit
GTCGTGACTGGGAAAACCCTGGCGGAGTGTCGTGATACCGTTATTTAGCT34770.11693837377970587No Hit
GTCGTGACTGGGAAAACCCTGGATGGATGTCGTGATCCACATAGCTAGCT34540.1161648383765039No Hit
GTCGTGACTGGGAAAACCCTGGTGTTGTGTCGTGATGCAACTCCCTAGCT34110.11471866349225673No Hit
GTCGTGACTGGGAAAACCCTGGACTAGAGTCGTGATGTAAACAGCTAGCT33890.11397876006310705No Hit
GTCGTGACTGGGAAAACCCTGGTCCCTTGTCGTGATTCATTTTTATAGCT33830.11377696821879349No Hit
GTCGTGACTGGGAAAACCCTGGAAATAGGTCGTGATCCATATAGCTAGCT32780.11024561094330623No Hit
GTCGTGACTGGGAAAACCCTGGGTAATCGTCGTGATAAGCATACTTAGCT32580.10957297146226105No Hit
GTCGTGACTGGGAAAACCCTGGGAAGGCGTCGTGATGGCAAGAGCTAGCT32380.10890033198121588No Hit
GTCGTGACTGGGAAAACCCTGGATGCGAGTCGTGATCCTAACTGATAGCT32080.10789137275964808No Hit
GTCGTGACTGGGAAAACCCTGGCCCCGCGTCGTGATTGGAGATCCTAGCT31880.1072187332786029No Hit
GTCGTGACTGGGAAAACCCTGGAATGTAGTCGTGATGTTGGCACTTAGCT31410.10563803049814671No Hit
GTCGTGACTGGGAAAACCCTGGCGCAACGTCGTGATAGTACTTAGTAGCT31310.10530171075762412No Hit
GTCGTGACTGGGAAAACCCTGGCAATGAGTCGTGAAGTTATAGCCTAGCT31280.10520081483546732No Hit
GTCGTGACTGGGAAAACCCTGGGTTAAGGTCGTGATGCATCCCCCTAGCT31230.10503265496520604No Hit
GTCGTGACTGGGAAAACCCTGGTATCCCGTCGTGATCTAACGCAATAGCT31210.10496539101710152No Hit
GTCGTGACTGGGAAAACCCTGGACCCTGGTCGTGATGCGCACCACTAGCT31120.10466270325063119No Hit
GTCGTGACTGGGAAAACCCTGGCCAGCAGTCGTGATAGGGAAAGCTAGCT31050.10442727943226539No Hit
GTCGTGACTGGGAAAACCCTGGACAATAGTCGTGATAGTTATAACTAGCT31020.10432638351010859No Hit
GTCGTGACTGGGAAAACCCTGGGATCTCGTCGTGATACCCTCTCCTAGCT30910.10395643179553374No Hit
GTCGTGACTGGGAAAACCCTGGTCATCCGTCGTGATAAACATAGCTAGCT30690.10321652836638402No Hit
GTCGTGACTGGGAAAACCCTGGTCCGGCGTCGTGATATGCAGAAATAGCT30430.1023420970410253No Hit
GTCGTGACTGGGAAAACCCTGGCGCTTGGTCGTGATAATATGTATTAGCT30430.1023420970410253No Hit
GTCGTGACTGGGAAAACCCTGGAAATTAGTCGTGATATTATGTAGTAGCT30200.10156856163782332No Hit
GTCGTGACTGGGAAAACCCTGGTGATCCGTCGTGATGTTAACACCTAGCT30170.10146766571566654No Hit
GTCGTGACTGGGAAAACCCTGGATCAGGGTCGTGATTGAGTTGATTAGCT30060.10109771400109169No Hit
GTCGTGACTGGGAAAACCCTGGGCATTTGTCGTGATCAGTCGTCGTAGCT30000.10089592215677813No Hit
GTCGTGACTGGGAAAACCCTGGCTAGGGGTCGTGATACCTTGTAGTAGCT29830.10032417859788972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG2801400.0241.979344
CGTGACT2766350.0241.83553
TGACTGG2801000.0241.824585
TCGTGAC2805650.0241.255312
GACTGGG2832050.0240.554146
ACGTGAC4150.0233.80192
TGGGAAA2995350.0222.225779
GGTGACT42500.0213.898453
CGTGACA36850.0213.143883
TTGTGAC1800.0208.879262
ACTGGGA3247400.0208.457187
CTGGGAA3292550.0204.754828
CGTGGCT23250.0204.36123
TCGTGGC20750.0198.147112
GACGGGG30650.0194.749536
TCGGGAC19800.0192.340422
CGGGACT19050.0192.153053
ACGGGGA29000.0183.263217
TGGTGAC61600.0179.958192
ACCGGGA6650.0171.125637
Produced by FastQC (version 0.11.5)