FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541380
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTTTCAAGTCGTGATAGCACAGGGGCCAA310555.73626657800436No Hit
GTCGTGACTGGGAAAACCCTGGGTCAACGTCGTGATAATGCGCTCGCCAA271105.007573238760205No Hit
GTCGTGACTGGGAAAACCCTGGCCTATGGTCGTGATCGGAATGAGGCCAA219224.049281465883483No Hit
GTCGTGACTGGGAAAACCCTGGACACTCGTCGTGATGAAGTGGGAGCCAA152522.817244818796409No Hit
GTCGTGACTGGGAAAACCCTGGATTGGTGTCGTGATGTGAGTAAGGCCAA132502.447449111529794No Hit
GTCGTGACTGGGAAAACCCTGGACTCAAGTCGTGATACTATCTCTGCAAG114472.1144113192212495No Hit
GTCGTGACTGGGAAAACCCTGGTCTGCCGTCGTGATAGATACATGGCCAA113052.0881820532712694No Hit
GTCGTGACTGGGAAAACCCTGGTTTCGCGTCGTGATTAGCGTTATGCCAA91041.6816284310465848No Hit
GTCGTGACTGGGAAAACCCTGGAAGTTCGTCGTGATCGGGATTTTGCCAA89951.6614946987328678No Hit
GTCGTGACTGGGAAAACCCTGGGACCCCGTCGTGATAAACGGGGCGCCAA84171.554730503528021No Hit
GTCGTGACTGGGAAAACCCTGGGCGGATGTCGTGATCCAAAGTAAGCCAA69011.274705382540914No Hit
GTCGTGACTGGGAAAACCCTGGATACGAGTCGTGATTAAGGGAGCGCCAA68721.2693487014666223No Hit
GTCGTGACTGGGAAAACCCTGGGATATTGTGTGATCCAACGGAAAGCCAA63691.1764379917987364No Hit
GTCGTGACTGGGAAAACCCTGGCGCTCAGTCGTGATCGGGACTAGGCCAA62091.1468838893198863No Hit
GTCGTGACTGGGAAAACCCTGGCATTTTGTCGTGATATATGATCTGCCAA61641.1385717979977097No Hit
GTCGTGACTGGGAAAACCCTGGGAACGCGTCGTGATTAGAGTAGTGCCAA59291.0951642099818981No Hit
GTCGTGACTGGGAAAACCCTGGTATCGTGTCGTGATGTCTGCGAGGCCAA59071.0911005208910562No Hit
GTCGTGACTTGGAAAACCCTGGACACTCGTCGTGATGAAGTGGGAGCCAA58731.0848202741143005No Hit
GTCGTGACTGGGAAAACCCTGGCGAACCGTCGTGATAAGGAGTTCGCCAA55451.0242343640326572No Hit
GTCGTGACTGGGAAAACCCTGGTCGCTTGTCGTGATCTTAAGAAGGCCAA53930.9961579666777495No Hit
GTCGTGACTGGGAAAACCCTGGCGTGATGTCGTGATCTAGGCAGTGCCAG53020.9793490708929034No Hit
GTCGTGACTGGGAAAACCCTGGTAATGCGTCGTGATGCGCACCAGGCCAA52680.9730688241161476No Hit
GTCGTGACTGGGAAAACCCTGGAAATTTGTCGTGATTGACTCCACGCCAA52380.9675274299013633No Hit
GTCGTGACTGGGAAAACCCTGGATTCTTGTCGTGATCACAACGGAGCCAA51400.9494255421330674No Hit
GTCGTGACTGGGAAAACCCTGGATCTGTGTCGTGATGCCAGGGAAGCCAA48900.9032472570098636No Hit
GTCGTGACTGGGAAAACCCTGGCTCTATGTCGTGATTATGTTATGGCCAA46810.8646422106468654No Hit
GTCGTGACTGGGAAAACCCTGGACGTCTGTCGTGATCGGCGCGTTGCCAA45940.8485721674239906No Hit
GTCGTGACTGGGAAAACCCTGGTGTCTGGTCGTGATTAAGCTCTAGCCAA42860.7916805201522036No Hit
GTCGTGACTGGGAAAACCCTGGTGGTTAGTCGTGATTGCAGGTGTGCCAA42530.7855849865159408No Hit
GTCGTGACTGGGAAAACCCTGGAAGCCAGTCGTGATTAATCCCTAGCCAA40010.7390372751117514No Hit
GTCGTGACTGGGAAAACCCTGGTGCCTAGTCGTGATGCACACGTTGCCAA37620.6948908345339687No Hit
GTCGTGACTGGGAAAACCCTGGTTAACCGTCGTGATGAATATACCGCCAA37410.6910118585836196No Hit
GTCGTGACTGGGAAAACCCTGGTGTATGGTCGTGATCGCTGAGACGCCAA35990.6647825926336399No Hit
GTCGTGACTGGGAAAACCCTGGTGCCGAGTCGTGATCCTCAAGATGCCAA35980.6645978794931472No Hit
GTCGTGACTGGGAAAACCCTGGTGCGGCGTCGTGATCCACTTTGTGCCAA34960.64575713916288No Hit
GTCGTGACTGGGAAAACCCTGGCGCACTGTCGTGATCAGCTTACGGCCAA34730.6415087369315453No Hit
GTCGTGACTGGGAAAACCCTGGCGCTAGGTCGTGATATGAATAATGCCAA33900.6261775462706417No Hit
GTCGTGACTGGGAAAACCCTGGCAGCGTGTCGTGATGTAGATGCCGCCAA32980.6091839373453027No Hit
GTCGTGACTGGGAAAACCCTGGGAACCAGTCGTGATCTGGCGAGAGCCAA31400.579999261147438No Hit
GTCGTGACTGGGAAAACCCTGGTCATCAGTCGTGATATAAATTACGCCAA31260.5774132771805387No Hit
GTCGTGACTGGGAAAACCCTGGGACCTCGTCGTGATCAAGATACAGCCAA29450.5439801987513392No Hit
GTCGTGACTGGGAAAACCCTGGCCCTTCGTCGTGATAGCATGATTGCCAA27300.504266873545384No Hit
GTCGTGACTGGGAAAACCCTGGGCGCTAGTCGTGATGTTTGGTTTGCCAA26540.49022867486793015No Hit
GTCGTGACTGGGAAAACCCTGGTTAACTGTCGTGATATGGTGTTTGCCAA24740.4569803095792234No Hit
GTCGTGACTGGGAAAACCCTGGGGTATTGGCACCACCGTGGGCCGGCCAA24680.45587203073626664No Hit
GTCGTGACTGGGAAAACCCTGGGATATTGTCTGATCCAACGGAAAGCCAA24570.4538401861908456No Hit
GTCGTGACTGGGAAAACCCTGGCAACTCGTCGTGATAGGGTCACGGCCAA24570.4538401861908456No Hit
GTCGTGACTGGGAAAACCCTGGTACCACGTCGTGATTATGGTTCTGCCAA23540.4348147327200857No Hit
GTCGTGACTGGGAAAACCCTGGTACGTAGTCGTGATTGCCATTACGCCAA23470.433521740736636No Hit
GTCGTGACTGGGAAAACCCTGGATGCGCGTCGTGATCAAAATTGAGCCAA22540.41634341867080427No Hit
GTCGTGACTGGGAAAACCCTGGTAATTCGTCGTGATCCGGAAACCGCCAA22470.41505042668735453No Hit
GTCGTGACTGGGAAAACCCTGGTATCGCGTCGTGATCTGAACAGGGCCAA22450.41468100040636896No Hit
GTCGTGACTGGGAAAACCCTGGAGGAAAGTCGTGATTGAGAGGTGGCCAA21840.40341349883630717No Hit
GTCGTGACTGGGAAAACCCTGGGCGGATGTCGTGATGGATGATCCGCCAA19350.35741992685359636No Hit
ATCAATGAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTGCCAA18880.3487384092504341No Hit
GTCGTGACTGGGAAACCCCTGGTTTCAAGTCGTGATAGCACAGGGGCCAA18250.3371014813993867No Hit
GTCGTGACTGGGAAAACCCTGGAACCTCGTCGTGATCAGGTGGCTGCCAA17320.319923159333555No Hit
GTCGTGACTGGGAAACCCCTGGGTCAACGTCGTGATAATGCGCTCGCCAA16080.29701872991244593No Hit
GTCGTGACTGGGAAAACCCTGGTCTATAGTCGTGATCAGGCGGGAGCCAA15880.2933244671025897No Hit
GTCGTGACTGGGAAAACCCTGGACGGCGGTCGTGATACCGAGAGAGCCAA15660.28926077801174777No Hit
ATGAATGAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTGCCAA14620.270050611400495No Hit
GTCGTGACTGGGAAAACCCTGGATTCCTGTCGTGATATAGATAGGGCCAA13820.2552735601610699No Hit
GTCGACTCTGAAAACCCTCGGTCAACGTCGTGATAATGCGCTCTTGCCAA11190.2066940042114596No Hit
GTCGTGACTGGGAAAACCCTGGATACTCGTCGTGATAGGTCAAACGCCAA10970.20263031512061766No Hit
GTCGTGACTGGGAAAACCCGGGTTTCAAGTCGTGATAGCACAGGGGCCAA10850.2004137574347039No Hit
GTCGTGACTGGGAAAACCCTGGGCCCCCGTCGTGATCAAATCACCGCCAA10740.19838191288928295No Hit
GTCGTGACTGGGAACACCCTGGTTTCAAGTCGTGATAGCACAGGGGCCAA10590.19561121578189072No Hit
GTCGACTCTGAAAACCCTGGGTCAACGTCGTGATAATGCGCTCTTGCCAA10130.18711441131922124No Hit
GTCGTGACTGGGAAACCCCTGGCCTATGGTCGTGATCGGAATGAGGCCAA10010.1848978536333075No Hit
GTCGTGACTGGGAAAGCCCTGGTTTCAAGTCGTGATAGCACAGGGGCCAA9910.18305072222837931No Hit
GTCGTGACTGGGAAAACCCTGGTGCAGTGTCGTGATCCCCGGTGCGCCAA9400.17363035206324579No Hit
GTCGACTCTGAAAACCCTGTGTCAACGTCGTGATAATGCGCTCTTGCCAA9010.166426539584026No Hit
GTCGTGACTGGGAACACCCTGGGTCAACGTCGTGATAATGCGCTCGCCAA8720.16106985850973438No Hit
GTCGTGACTGGGAAACCCCTGGACACTCGTCGTGATGAAGTGGGAGCCAA8700.16070043222874875No Hit
GTCGTGACTGGGAAAGCCCTGGGTCAACGTCGTGATAATGCGCTCGCCAA7840.1448151021463667No Hit
GTCGTGACTGGGAAAACCCTGGCGTGATGTCGTGATCTAGGCAGTGCCAA7500.138534855369611No Hit
GTCGTGACTGGGAAAACCCTGGATTTATGTCGTGATCGTACCCCGGCCAA7310.1350253057002475No Hit
GTCGTGACTGGGAAAACCCTGGCTCTGTGTCGTGATCAATCCACTGCCAA7170.1324393217333481No Hit
GTCGTGACTGGGAAAACCCTGGCTATTTGTCGTGATGCGGTAGGGGCCAA6990.12911448520447746No Hit
GTCGTGACTGGGAAAACCCTGGTGTAACGTCGTGATGCAGCGGGAGCCAA6710.12394251727067863No Hit
GTCGTGACTGGGAAAACCCTGGTGCATGGTCGTGATTTCTACTTTGCCAA6710.12394251727067863No Hit
GTCGTGACTGGGAAAACCCGGGGTCAACGTCGTGATAATGCGCTCGCCAA6660.12301895156821456No Hit
GTCGTGACTGGGAAAACCCTGGCCGAGAGTCGTGATGCCTGGGGTGCCAA6640.12264952528722893No Hit
GTCGTGACTGGGAAAACCCTGGGTACCGGTCGTGATCAGGTATGCGCCAA6490.11987882817983671No Hit
GTCGACTCTGAAAACCCTCTGTCAACGTCGTGATAATGCGCTCTTGCCAA6490.11987882817983671No Hit
GTCGTGACTGGGAACACCCTGGCCTATGGTCGTGATCGGAATGAGGCCAA6430.11877054933687983No Hit
GTCGTGACTGGGAAACCCCTGGTCTGCCGTCGTGATAGATACATGGCCAA6280.11599985222948761No Hit
ATCAATGATACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTGCCAA6240.11526099966751634No Hit
GTCGTGACTGGGAAAACCCTGGAGTGTCGTCGTGATACGGTTTAGGCCAA6190.11433743396505228No Hit
GTCGTGACTGGAAAACCCTGGGACGGGGTCGTGATATCAATTACTGCCAA6000.1108278842956888No Hit
GTCGTGACTGGGAAAACCCTGGCTCCGCGTCGTGATACAATGTAGGCCAA5630.10399349809745466No Hit
GTCGTGACTGGGAACACCCTGGACACTCGTCGTGATGAAGTGGGAGCCAA5600.1034393586759762No Hit
GTCGTGACTGGGAAACCCCTGGTTTCGCGTCGTGATTAGCGTTATGCCAA5460.10085337470907679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC498000.0242.445242
CGTGACT496800.0242.332983
GTTACTT100.0015259247242.292084
GTGACTG495700.0242.218774
TGCGAAA251.7298589E-9242.18019
TGACTGG496100.0241.980745
GACTGGG497100.0241.424966
ACTGGGA494800.0241.127767
TGGGAAA467450.0240.988489
GTGACTT8550.0240.875174
ATGAGAC5250.0239.917955
TCAATGA4400.0239.738312
TGAGACA5550.0237.81656
ATGATAC1800.0235.49645
TCTGAAA7150.0235.40587
CTGGGAA508650.0235.085858
GTCGACT7850.0234.792921
CGTGGCT6500.0233.059393
TGAAAAC7900.0231.450599
GGTGACT7250.0229.008973
Produced by FastQC (version 0.11.5)