FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58997
Sequences flagged as poor quality0
Sequence length248
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGCACGTGTCGTGATGGAATATGACGATG2560.43392036883231355No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTACATCGGGGGGGAGCGATG2140.3627303083207621No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTACATCGACCACAAGCGATG1350.2288251945014153No Hit
GTCGTGACTGGGAAAACCCTGGGGCTGCGTCGTGATCGATCATTGCGATG1320.22374019017916166No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTACATCGGCCACAAGCGATG870.14746512534535655No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTACATCGACCGCAAGCGATG710.12034510229333695No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTACATCGGGCGGGAGCGATG710.12034510229333695No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTACATCGGGGGCGAGCGATG700.11865010085258573No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTACATCGACCGCGAGCGATG680.11526009797108328No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTACATCGTCCACAAGCGATG670.11356509653033206No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTACATCGGCCGCGAGCGATG640.10848009220807839No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTACATCGAGGGGGAGCGATG640.10848009220807839No Hit
GTCGTGACTGGGAAAACCCTGGACAGGGGTCGTGATGCCCGGGTTCGATG640.10848009220807839No Hit
GTCGTGACTGGGAAAACCCTGGTGTGATGTCGTGATTGTTAGGATCGATG610.10339508788582472No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGAC100.0015138015242.75492
TCGTAAC100.0015138015242.75492
GCGTGGC100.0015138015242.75492
CGTGACA750.0242.754883
TTGACTG100.0015176651242.548484
ACCGGGG151.579336E-5242.342397
GACTGGC100.0015215353242.342396
TCGTGAC54200.0241.63522
CGTGACT53450.0241.619463
ACTGGGA53150.0238.694727
TGGGAAA51200.0238.589749
GTGACTG55000.0238.359014
GACTGGG53400.0238.031056
TGACTGG54550.0237.899835
CTGGGAA53650.0234.915458
ACTGGAA1300.0223.700677
GTGGTGA1050.0219.822481
GTGACAG900.0215.598624
TGACTGA700.0207.722065
ACAGGGA900.0201.951987
Produced by FastQC (version 0.11.5)