FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326723
Sequences flagged as poor quality0
Sequence length248
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGACGACGGTCGTGATTGACTCACTCAGTG8580.26260777478169584No Hit
GTCGTGACTGGGAAAACCCTGGCCCTCAGTCGTGATAACCATACCCAGTG8450.2586288691031853No Hit
GTCGTGACTGGGAAAACCCTGGCCGCACGTCGTGATTTCTGCGGCCAGTG8020.24546787339734272No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGGGTCGTGATAAGAAGTTGCAGTG7520.230164390018456No Hit
GTCGTGACTGGGAAAACCCTGGGCCATCGTCGTGATTGGACATTTCAGTG7390.22618548433994545No Hit
GTCGTGACTGGGAAAACCCTGGAGTATTGTCGTGATGATGCCATTCAGTG7240.22159443932627942No Hit
GTCGTGACTGGGAAAACCCTGGATGAAAGTCGTGATAGGACATACCAGTG7030.21516697630714704No Hit
GTCGTGACTGGGAAAACCCTGGGTCTGGAGTCGTGATCGGTGTATCAGTG6900.21118807062863648No Hit
GTCGTGACTGGGAAAACCCTGGTTCTCGGTCGTGATCTTAGGACTCAGTG6820.20873951328801463No Hit
GTCGTGACTGGGAAAACCCTGGCCTCGAGTCGTGATCCTGAGTACCAGTG6720.20567881661223728No Hit
GTCGTGACTGGGAAAACCCTGGACACACGTCGTGATGACTAGGAACAGTG6700.20506667727708183No Hit
GTCGTGACTGGGAAAACCCTGGGTCCGTGTCGTGATAGCATATGTCAGTG6620.20261811993645992No Hit
GTCGTGACTGGGAAAACCCTGGCTACGGGTCGTGATATAGTTTATCAGTG6380.19527244791459433No Hit
GTCGTGACTGGGAAAACCCTGGCAGCATGTCGTGATTTGAACATCCAGTG6370.19496637824701657No Hit
GTCGTGACTGGGAAAACCCTGGTACCATGTCGTGATGAAAATTACCAGTG6350.19435423891186113No Hit
GTCGTGACTGGGAAAACCCTGGTCGCAGGTCGTGATGCAAATGGCCAGTG6260.1915996119036615No Hit
GTCGTGACTGGGAAAACCCTGGTCTAAAGTCGTGATCAGATATTAACAGT6150.18823284556030642No Hit
GTCGTGACTGGGAAAACCCTGGACTTCGGTCGTGATGCCCTGTCCCAGTG5810.17782647686266348No Hit
GTCGTGACTGGGAAAACCCTGGCATTCCGTCGTGATGGAAAGGAGCAGTG5740.17568398918961933No Hit
GTCGTGACTGGGAAAACCCTGGACCCATGTCGTGATAACAAGTCTCAGTG5580.1707868745083756No Hit
GTCGTGACTGGGAAAACCCTGGGGCCGAGTCGTGATCTTACCTCACAGTG5520.16895045650290919No Hit
GTCGTGACTGGGAAAACCCTGGCCATTAGTCGTGATTTAAAACCGCAGTG5480.16772617783259827No Hit
GTCGTGACTGGGAAAACCCTGGTTCGCAGTCGTGATTCATAAACTCAGTG5450.16680796882986507No Hit
GTCGTGACTGGGAAAACCCTGGTCCCCAGTCGTGATAAGCAGTACCAGTG5370.16435941148924318No Hit
GTCGTGACTGGGAAAACCCTGGCCAACCGTCGTGATAGAAATACTCAGTG5340.16344120248650998No Hit
GTCGTGACTGGGAAAACCCTGGCTGAGAGGTGGCTTCAACTGGCTCAGTG5330.16313513281893224No Hit
GTCGTGACTGGGAAAACCCTGGAATTCAGTCGTGATCTAGACCCTCAGTG5240.16038050581073265No Hit
GTCGTGACTGGGAAAACCCTGGAGGTAGGTCGTGATTTGTGTAAACAGTG5110.1564016001322221No Hit
GTCGTGACTGGGAAAACCCTGGGCTTTGGTCGTGATGACAGTTATCAGTG4970.15211662478613383No Hit
GTCGTGACTGGGAAAACCCTGGTCGGGAGTCGTGATGGGTATTGTCAGTG4950.15150448545097836No Hit
GTCGTGACTGGGAAAACCCTGGTACGACGTCGTGATAGTTAGTTACAGTG4880.1493619977779342No Hit
GTCGTGACTGGGAAAACCCTGGGATCCCGTCGTGATCGTCAGAGCCAGTG4860.14874985844277874No Hit
GTCGTGACTGGGAAAACCCTGGCTTGCGGTCGTGATGCCTAGAGTCAGTG4730.1447709527642682No Hit
GTCGTGACTGGGAAAACCCTGGATGGACGTCGTGATGGGTACTCTCAGTG4730.1447709527642682No Hit
GTCGTGACTGGGAAAACCCTGGCCCTTAGTCGTGATCCAGATTCTCAGTG4650.14232239542364633No Hit
GTCGTGACTGGGAAAACCCTGGCCCTAAGTCGTGATTAATGTACTCAGTG4520.13834348974513577No Hit
GTCGTGACTGGGAAAACCCTGGAACGCAGTCGTGATCTCGAGGGCCAGTG4480.13711921107482486No Hit
GTCGTGACTGGGAAAACCCTGGTCAACCGTCGTGATTAGATTGGTCAGTG4470.13681314140724712No Hit
GTCGTGACTGGGAAAACCCTGGTATGAGGTCGTGATTTTACGTAACAGTG4450.13620100207209163No Hit
GTCGTGACTGGGAAAACCCTGGCCCGCGGTCGTGATAAAAGCTACCAGTG4440.13589493240451392No Hit
GTCGTGACTGGGAAAACCCTGGAAGGGAGTCGTGATCCGCACTTTCAGTG4350.1331403053963143No Hit
GTCGTGACTGGGAAAACCCTGGGGAAAAGTCGTGATCTTGAGGAGCAGTG4350.1331403053963143No Hit
GTCGTGACTGGGAAAACCCTGGACATAAGTCGTGATTACCCGTCACAGTG4330.13252816606115883No Hit
GTCGTGACTGGGAAAACCCTGGTTAGTTGTCGTGATACTTATGGGCAGTG4290.13130388739084792No Hit
GTCGTGACTGGGAAAACCCTGGGTTGCGGTCGTGATAGGCAGGTGCAGTG4190.12824319071507057No Hit
GTCGTGACTGGGAAAACCCTGGCTCGCTGTCGTGATGCTACGGATCAGTG4180.12793712104749283No Hit
GTCGTGACTGGGAAAACCCTGGAAACGGGTCGTGATGTGAACCCCCAGTG4120.12610070304202645No Hit
GTCGTGACTGGGAAAACCCTGGCCTTGTGTCGTGATATCGATTCTCAGTG4110.12579463337444868No Hit
GTCGTGACTGGGAAAACCCTGGGCCCTCGTCGTGATTAGGAGTTGCAGTG4100.12548856370687095No Hit
GTCGTGACTGGGAAAACCCTGGTGGCTCGTCGTGATATCTCTGTGCAGTG3970.1215096580283604No Hit
GTCGTGACTGGGAAAACCCTGGCTTTAAGTCGTGATTTCAGCGACCAGTG3920.11997930969047176No Hit
GTCGTGACTGGGAAAACCCTGGGGCCGAGTCGTGATGCTGTGAAACAGTG3870.11844896135258308No Hit
GTCGTGACTGGGAAAACCCTGGAAGTTCGTCGTGATTCATTTGGCACAGT3780.11569433434438348No Hit
GTCGTGACTGGGAAAACCCTGGTACTGTGTCGTGATGACAATCTCCAGTG3780.11569433434438348No Hit
GTCGTGACTGGGAAAACCCTGGCTATTGGTCGTGATCTCCTAGACCAGTG3760.115082195009228No Hit
GTCGTGACTGGGAAAACCCTGGGAGCGTGTCGTGATTATTAATACCAGTG3690.11293970733618386No Hit
GTCGTGACTGGGAAAACCCTGGCCCGTGGTCGTGATAAGGACTTGCAGTG3670.11232756800102839No Hit
GTCGTGACTGGGAAAACCCTGGTAAATTGTCGTGATCCAACGGAACAGTG3650.11171542866587293No Hit
GTCGTGACTGGGAAAACCCTGGCCATCAGTCGTGATGAAACTGATCAGTG3640.11140935899829518No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGGCTGAGTGTCCCGCGGGGCAGTG3640.11140935899829518No Hit
GTCGTGACTGGGAAAACCCTGGACACAGGTCGTGATCTGGACCCACAGTG3620.11079721966313971No Hit
GTCGTGACTGGGAAAACCCTGGGTATAGGTCGTGATGCTGACAACCAGTG3610.11049114999556199No Hit
GTCGTGACTGGGAAAACCCTGGTATGATGTCGTGATTTGACGGCACAGTG3590.10987901066040653No Hit
GTCGTGACTGGGAAAACCCTGGAACAAAGTCGTGATAAGAAGAGTCAGTG3580.10957294099282879No Hit
GTCGTGACTGGGAAAACCCTGGAACCAGGTCGTGATTGAAATTTTCAGTG3560.10896080165767331No Hit
GTCGTGACTGGGAAAACCCTGGTTGTAGGTCGTGATGGCGATATGCAGTG3560.10896080165767331No Hit
GTCGTGACTGGGAAAACCCTGGCGATTCGTCGTGATTAGAATGGCCAGTG3550.10865473199009558No Hit
GTCGTGACTGGGAAAACCCTGGTCAGCAGTCGTGATATCGTGGACCAGTG3530.10804259265494011No Hit
GTCGTGACTGGGAAAACCCTGGCTTTGAGTCGTGATCATTAGAAGCAGTG3460.10590010498189599No Hit
GTCGTGACTGGGAAAACCCTGGTTACGTGTCGTGATACGGTCACCCAGTG3450.10559403531431824No Hit
GTCGTGACTGGGAAAACCCTGGACCATTGTCGTGATATTACAAATCAGTG3390.10375761730885184No Hit
GTCGTGACTGGGAAAACCCTGGTCAGAAGTCGTGATTTCCAATAACAGTG3360.10283940830611864No Hit
GTCGTGACTGGGAAAACCCTGGCACGGAGTCGTGATCCCCGCGACCAGTG3360.10283940830611864No Hit
GTCGTGACTGGGAAAACCCTGGGATTATGTCGTGATGTATTCTCCCAGTG3330.10192119930338543No Hit
GTCGTGACTGGGAAAACCCTGGCTCTCCGTCGTGATTGGTTGGATCAGTG3330.10192119930338543No Hit
GTCGTGACTGGGAAAACCCTGGCTCGCAGTCGTGATTGTATTTACCAGTG3330.10192119930338543No Hit
GTCGTGACTGGGAAAACCCTGGCAACCAGTCGTGATGTTAAGGTGCAGTG3320.10161512963580771No Hit
GTCGTGACTGGGAAAACCCTGGATAGCCGTCGTGATCACTATGAACAGTG3300.10100299030065224No Hit
GTCGTGACTGGGAAAACCCTGGTGGAAAGTCGTGATGTCAATGTACAGTG3290.10069692063307449No Hit
GTCGTGACTGGGAAAACCCTGGCACCAAGCGTGATCCGAGCTCCCCAGTG3280.10039085096549677No Hit
GTCGTGACTGGGAAAACCCTGGGGATCCGTCGTGATGCAGGAGGGCAGTG3270.10008478129791903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC309650.0241.50142
CGTGACT310800.0239.600853
TGACTGG308950.0239.166535
TGGGAAA293050.0239.133139
GTGACTG311350.0239.065434
ACTGGGA306650.0237.763377
GACTGGG308200.0237.078126
CTGGGAA309650.0235.498938
GTGACTT1550.0187.491674
CGTGGCT3950.0183.988073
TGACTGA3300.0183.382975
TCGTGGC3450.0165.08742
GTCGTGG3500.0162.828841
CATGACT150.005135772161.500643
TGGGAAC14100.0157.943479
CTCGTGA402.8443537E-8151.569411
ACTGGAA5600.0151.290947
TGGAACC4050.0149.423169
AGCGACA650.0149.237561
GGTGACT2500.0140.505553
Produced by FastQC (version 0.11.5)