FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72143
Sequences flagged as poor quality0
Sequence length248
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTCCCCAGTCGTGATGGAGTGACAACTTG12741.7659370971542632No Hit
GTCGTGACTGGGAAAACCCTGGGGGCAAGTCGTGATTTGCGTCCCACTTG11181.5496999015843533No Hit
GTCGTGACTGGGAAAACCCTGGTTCGGTGTCGTGATCTAATTCTTACTTG8791.2184134288842992No Hit
GTCGCGACTGGGAAAACCCTGGTTGAGAGTCGTGATACCTCATTTACTTG8691.2045520701939203No Hit
GTCGTGACTGGGAAAACCCTGGACCGCCGTCGTGATTGATAGCCCACTTG7461.0340573583022608No Hit
GTCGTGACTGGGAAAACCCTGGCTCGATGTCGTGATGGCTAAGACACTTG7351.0188098637428442No Hit
GTCGTGACTGGGAAAACCCTGGAATCAGGTCGTGATGTCCAAAGCACTTG7231.0021762333143895No Hit
GTCGTGACTGGGAAAACCCTGGTACTTGGTCGTGATGCGTATACAACTTG6090.8441567442440708No Hit
GTCGTGACTGGGAAAACCCTGGCAACTAGTCGTGATGGCCGAGGAACTTG5500.7623747279708357No Hit
GTCGTGACTGGGAAAACCCTGGCTGGTAGTCGTGATGAACAGAAGACTTG5420.7512856410185327No Hit
GTCGTGACTGGGAAAACCCTGGAAAGGCGTCGTGATTTACACTCTACGTA4510.6251472769360853No Hit
GTCGTGACGGGAAAACCCTGGTTGAGAGTCGTGATACCTCATTTGACTTG4410.6112859182457064No Hit
GTCGTGACTGGGAAAACCCTGGTATTAGGTCGTGATGAGTGCACCACTTG4360.604355238900517No Hit
GTCGTGACTGGGAAAACCCTGGACATGTGTCGTGATCGCTGTGACACTTG4230.5863354726030245No Hit
GTCGTGACTGGGAAAACCCTGGGTTTCAGTCGTGATTGCGCGAGAACTTG4110.56970184217457No Hit
GTCGTGACTGGGAAAACCCTGGCGCTGAGTCGTGATCTATTGTCCACTTG4010.5558404834841911No Hit
GTCGTGACTGGGAAAACCCTGGGCGCCTGTCGTGATGCTAGTTATACTTG4000.5544543476151532No Hit
GTCGTGACTGGGAAAACCCTGGGAGGTGGTCGTGATGTTAATGGCACTTG3670.508711863936903No Hit
GTCGTGACTGGGAAAACCCTGGATCTGGGTCGTGATGTAGAGAGTACTTG3560.4934643693774864No Hit
GTCGTGACTGGGAAAACCCTGGTAGGCTGTCGTGATCCGAGGAGCACTTG3510.48653369003229696No Hit
GTCGTGACTGGGAAAACCCTGGATAAAAGTCGTGATAGGACACCCACTTG3460.47960301068710753No Hit
GTCGTGACTGGGAAAACCCTGGCACAAAGTCGTGATACGTACAAAACTTG3400.47128619547288025No Hit
GTCGTGACTGGGAAAACCCTGGTTCGTTGTCGTGATCTCGAGCAAACTTG3340.4629693802586529No Hit
GTCGTGACTGGGAAAACCCTGGCGGCTCGTCGTGATTGATATTCGACTTG3290.45603870091346355No Hit
GTCGTGACTGGGAAAACCCTGGATATAAGTCGTGATGATCTTGACACTTG3250.450494157437312No Hit
GTCGTGACTGGGAAAACCCTGGACAGATGTCGTGATGCGAGTCTCACTTG3060.4241575759255922No Hit
GTCGTGACTGGGAAAACCCTGGTAGTTCGTCGTGATCGCAAGACCACTTG3050.4227714400565543No Hit
GTCGTGACTGGGAAAACCCTGGGGCTACGTCGTGATAAAACCTTTACTTG2970.4116823531042512No Hit
GTCGTGACTGGGAAAACCCTGGGAATCCGTCGTGATGCATAAGCCACTTG2720.3770289563783042No Hit
GTCGTGACGGGAAAACCCTGGTTGAGAGTCCTGATACCTCATTTGACTTG2660.3687121411640769No Hit
GTCGTGACTGGGAAAACCCTGGTTATTAGTCGTGATCCATATAGCACTTG2630.36455373355696324No Hit
GTCGTGACTGGGAAAACCCTGGTTCATCGTCGTGATTATATGTAGACTTG2600.36039532594984963No Hit
GTCGTGACTGGGAAAACCCTGAGCCTGGTCCTGATCTAATGAAGAACTTG2500.3465339672594708No Hit
GTCGTGACTGGGAAAACCCTGGCTTGTGGTCGTGATCCATTTCATACTTG2470.3423755596523571No Hit
GTCGTGACTGGGAAAACCCTGGCACTCCGTCGTGATTGCAAGATGACTTG2450.33960328791428135No Hit
GTCGTGACTGGGAAAACCCTGGAACCCTGTCGTGATACGAAATTGACTTG2420.33544488030716774No Hit
GTCGTGACTGGGAAAACCCTGGAGTAACGTCGTGATTTCGATTCTACTTG2390.33128647270005407No Hit
GTCGTGACTGGGAAAACCCCTGCTGAGGTGCAACTACTCATCGTCACTTG2240.31049443466448584No Hit
GTCGTGACTGGGAAAACCCTGGGGTTGAGTCGTGATCCAACTGAGACTTG2190.3035637553192964No Hit
GTCGTGACTGGGAAAACCCTGGTGAAATGTCGTGATCAGTGCAAAACTTG2190.3035637553192964No Hit
GTCGTGACTGGGAAAACCCTGGCTGAAAGTCGTGATCCGAGGTGAACTTG2170.30079148358122065No Hit
GTCGTGACTGGGAAAACCCTGGCGACGGGTCGTGATGCAAGTGGCACTTG2160.29940534771218275No Hit
GTCGTGACTGGGAAAACCCTGGCTGGTCGTCGTGATGTCTGCGGGACTTG2160.29940534771218275No Hit
GTCGTGACTGGGAAAACCCTGGAAAAGCGTCGTGATAACAATTAGACTTG2120.2938608042360312No Hit
GTCGTGACTGGGAAAACCCTGGCTCAACGTCGTGATAAGAAGCACACTTG2110.2924746683669933No Hit
GTCGTGACTGGGAAAACCCTGGTCTCCAGTCGTGATTACACAGGGACTTG1970.273068766200463No Hit
GTCGTGACTGGGAAAACCCTGGGCTGATGTCGTGATGCGAAGAAAACTTG1930.2675242227243114No Hit
GTCGTGACTGGGAAAACCCTGGTGACACGTCGTGATAATTACTCAACTTG1920.26613808685527357No Hit
GTCGTGACTGGGAAAACCCTGGAAACGTGTCGTGATAAGTACAGCACTTG1910.26475195098623566No Hit
GTCGTGACTGGGAAAACCCTGGTAGAGGGTCGTGATGGCGGGAGCACTTG1870.25920740751008414No Hit
GTCGTGACTGGGAAAACCCTGGGGATCCGTCGTGATGGCTAGGGGACTTG1860.25782127164104623No Hit
GTCGTGACTGGGAAAACCCTGGGATAAGGTCGTGATACCGAGAGGACTTG1850.2564351357720084No Hit
GTCGTGACTGGGAAAACCCTGGCTGATGGTCGTGATCTAACGAGCACTTG1820.2522767281648947No Hit
GTCGTGACTGGGAAAACCCTGAGCCTGGTCGTGATCTAATGAAGAACTTG1780.2467321846887432No Hit
GTCGTGACTGGGAAAACCCTGGCCGTTCGTCGTGATCGGTCACCCACTTG1720.2384153694745159No Hit
GTCGTGACTGGGAAAACCCTGGTCCAAAGTCGTGATAGGTGCGGCACTTG1710.23702923360547798No Hit
GTCGTGACTGGGAAAACCCTGGCTAGGGGTCGTGATCGAATCACTACTTG1680.23287082599836437No Hit
GTCGTGACTGGGAAAACCCTGGCTGCCAGTCGTGATGCTAAGATCACTTG1660.2300985542602886No Hit
GTCGTGACTGGGAAAACCCTGGGTAGATGTCGTGATATAAGTGGGACTTG1650.2287124183912507No Hit
GTCGTGACTGGGAAAACCCTGGACGACGGTCGTGATCTAGGGGGTACTTG1650.2287124183912507No Hit
GTCGTGACTGGGAAAACCCTGGTGCACGGTCGTGATAGATTCACTACTTG1630.22594014665317494No Hit
GTCGTGACTGGGAAAACCCTGGCTAGTCGTCGTGATCGCGCGGAGACTTG1630.22594014665317494No Hit
GTCGTGACTGGGAAAACCCTGGACTGAGGTCGTGATGAAGAGTACACTTG1550.21485105970087187No Hit
GTCGTGACTGGGAAAACCCTGGTATGTGGTCGTGATGGCAGAGGGACTTG1540.21346492383183402No Hit
GTCGTGACTGGGAAAACCCTGGGTATGCGTCGTGATGCCTGCACTACTTG1510.20930651622472035No Hit
GTCGTGACTGGGAAAACCCTGGTTAGTAGTCGTGATGCCGGCAGTACTTG1500.20792038035568247No Hit
GTCGTGACTGGGAAAACCCTGGAATGACGTCGTGATACATACGGAACTTG1480.2051481086176067No Hit
GTCGTGACTGGGAAAACCCTGGTTTGTAGTCGTGATTGGGACCAGACTTG1480.2051481086176067No Hit
GTCGTGACTGGGAAAACCCTGGAGTATGGTCGTGATCCGAAGTGTACTTG1480.2051481086176067No Hit
GTCGTGACTGGGAAAACCCTGGTTTTCTGTCGTGATTAATAAACCACTTG1470.2037619727485688No Hit
GTCGTGACTGGGAAAACCCTGGTTCAGGGTCGTGATATTAGGAGTACTTG1440.1996035651414552No Hit
GTCGTGACTGGGAAAACCCTGGGCGTCCGTCGTGATGCCGAGTATACTTG1440.1996035651414552No Hit
GTCGTGACTGGGAAAACCCTGGCCATCAGTCGTGATATGGTCGTTACTTG1390.19267288579626574No Hit
GTCGTGACTGGGAAAACCCTGGCGCCCTGTCGTGATGTAAGCATCACTTG1390.19267288579626574No Hit
GTCGTGACTGGGAAAACCCTGGATCGTGGTCGTATACAAAGGAACACTTG1370.18990061405818998No Hit
GTCGTGACTGGGAAAACCCTGGCAAATCGTCGTGATGCCCATCCGACTTG1370.18990061405818998No Hit
GTCGTGACTGGGAAAACCCTGGGTGACCGTCGTGATTTACACGGGACTTG1340.18574220645107634No Hit
GTCGTGACTGGGAAAACCCTGGGCCAATGTCGTGATAGTAACGACACTTG1330.18435607058203846No Hit
GTCGTGACTGGGAAAACCCTGGGAGAAAGTCGTGATAACTGAGATACTTG1330.18435607058203846No Hit
GTCGTGACTGGGAAAACCCTGGCCGTGAGTCGTGATTCGATCGATACTTG1300.18019766297492482No Hit
GTCGTGACTGGGAAAACCCTGGTACACAGTCGTGATACCAGGCTGACTTG1300.18019766297492482No Hit
GTCGTGACTGGGAAAACCCTGGTCTGCAGTCGTGATCAGAACAATACTTG1280.17742539123684903No Hit
GTCGTGACTGGGAAAACCCTGGGCAATTGTCGTGATCATGATTGGACTTG1270.17603925536781115No Hit
GTCGTGACTGGGAAAACCCTGGTTCTAGGTCGTGATGTGCTAGCTACTTG1260.17465311949877327No Hit
GTCGTGACTGGGAAAACCCTGGCATTAAGTCGTGATATAGGGGAAACTTG1240.1718808477606975No Hit
GTCGTGACTGGGAAAACCCTGGGCCATCGTCGTGATGAGAGGAAAACTTG1210.16772244015358387No Hit
GTCGTGACTGGGAAAACCCTGGCAGTTGGTCGTGATTAATATGAGACTTG1210.16772244015358387No Hit
GTCGTGACTGGGAAAACCCTGGGATTGAGTCGTGATCAAAGCGTTACTTG1200.166336304284546No Hit
GTCGTGACTGGGAAAACCCTGGCATACGGTCGTGATCTAAACCTGACTTG1200.166336304284546No Hit
GTCGTGACTGGGAAAACCCTGGGTCCACAGTCGTGATCACAATTCACTTG1180.1635640325464702No Hit
GTCGTGACTGGGAAAACCCTGGGATATAGTCGTGATCATCTCTACACTTG1170.16217789667743232No Hit
GTCGTGACTGGGAAAACCCTGGCATATAGTCGTGATTCCAGTGGGACTTG1170.16217789667743232No Hit
GTCGTGACTGGGAAAACCCTGGTAACCGTCCTGATTGGCAGAAGCACTTG1160.16079176080839444No Hit
GTCGTGACTGGGAAAACCCTGGAACGTTGTCGTGATGTTGGCGCTACTTG1160.16079176080839444No Hit
GTCGTGACTGGGAAAACCCTGGTAACCGTCGTGATTGGCAGAAGCACTTG1140.15801948907031868No Hit
GTCGTGACTGGGAAAACCCTGGAATGTGGTCGTGATCTGCAGTTGACTTG1140.15801948907031868No Hit
GTCGTGACTGGGAAAACCCTGGACTCCCGTCGTGATACATACAGCACTTG1140.15801948907031868No Hit
GTCGTGACTGGGAAAACCCTGGGCCCGTGTCGTGATCCCTACACCACTTG1120.15524721733224292No Hit
GTCGTGACTGGGAAAACCCTGGGCCGTAGTCGTGATGAAAATAGGACTTG1050.1455442662489777No Hit
GTCGTGACTGGGAAAACCCTGGCCCTTTGTCGTGATAATGCTCCGACTTG1050.1455442662489777No Hit
GTCGTGACTGGGAAAACCCTGGGTACTCGTCGTGATTAACAGGTGACTTG1020.14138585864186407No Hit
GTCGTGACTGGGAAAACCCTGGGATCCGGTCGTGATTAAGTAACTACTTG1020.14138585864186407No Hit
GTCGTGACTGGGAAAACCCTGGGGTCAGGTCGTGATGAGTTGTGAACTTG1000.1386135869037883No Hit
GTCGTGACTGGGAAAACCCTGGTATAGCGTCGTGATTCCGATCCCACTTG990.13722745103475043No Hit
GTCGTGACGGGAAAACCCTTGTTGAGAGTCGTGATACCTCATTTGACTTG970.13445517929667467No Hit
GTCGTGACTGGGAAAACCCTGGTAAGCCGTCGTGATTGTCACTGAACTTG950.13168290755859888No Hit
GTCGTGACTGGGAAAACCCTGGTACTAGGTCGTGATTCTTACATTACTTG950.13168290755859888No Hit
GTCGTGACTGGGAAAACCCTGGTAATTTGTCGTGATGTCGAGTGGACTTG950.13168290755859888No Hit
GTCGTGACTGGGAAAACCCTGGGCCTGCGTCGTGATAAGAATTGAACTTG940.130296771689561No Hit
GTCGTGACTGGGAAAACCCTGGCCCCGCGTCGTGATGTCAAGGATACTTG940.130296771689561No Hit
GTCGTGACTGGGAAAACCCTGGACTCAAGTCGTGATCTAAAGTTGACTTG930.12891063582052312No Hit
GTCGTGACTGGGAAAACCCTGGACGGTCGTCATCTCAGCGACTGACTTGT920.12752449995148524No Hit
GTCGTGACTGGGAAAACCCTGGAAAACAGTCGTGATACGGGGAACACTTG920.12752449995148524No Hit
GTCGTGACTGGGAAAACCCTGGGTTTGAGTCGTGATGTCGAGGACACTTG910.12613836408244736No Hit
GTCGTGACTGGGAAAACCCTGGTGTGGCGTCGTGATAAGTTGCCGACTTG900.12475222821340948No Hit
GTCGTGACTGGGAAAACCCTGGATGGGTGTCGTGATTTCTGGAGCACTTG900.12475222821340948No Hit
GTCGTGACTGGGAAAACCCTGGAGTATAGTCGTGATATGTCCACAACTTG890.1233660923443716No Hit
GTCGTGACTGGGAAAACCCTGGCGTTGGGTCGTGATTAGGGCACCACTTG890.1233660923443716No Hit
GTCGTGACTGGGAAAACCCTGGTTCTCGGTCGTGATAAACATTTCACTTG870.12059382060629582No Hit
GTCGTGACTGGGAAAACCCTGGCGCTACGTCGTGATCTAAGGTCCACTTG870.12059382060629582No Hit
GTCGTGACTGGGAAAACCCTGGAACGCGGTCGTGATTCAAAACCCACTTG850.11782154886822006No Hit
GTCGTGACTGGGAAAACCCTGGAAAATGGTCGTGATGTCAATAGTACTTG850.11782154886822006No Hit
GTCGTGACTGGGAAAACCCTGGGCGTCCGTCGTGATGCCGAGTATCTTGT850.11782154886822006No Hit
GTCGTGACTGGGAAAACCCTGGTTAATTGTCGTGATTACTAGTACACTTG840.11643541299918218No Hit
GTCGTGACTGGGAAAACCCTGGGAAATCGTCGTGATTGAACCCCCACTTG840.11643541299918218No Hit
GTCGTGACTGGAAAACCCTGGTTGAGAGTCGTGATACCTCATTTGACTTG830.1150492771301443No Hit
GTCGTGACTGGGAAAACCCTGGAACTCCGTCGTGATATGGAAACGACTTG820.11366314126110641No Hit
GTCGTGACTGGGAAAACCCTGGAAAATTGTCGTGATACGAGTGGCACTTG800.11089086952303065No Hit
GTCGTGACTGGGAAAACCCTGGTGAATTGTCGTGATCATAGCAGTACTTG790.10950473365399277No Hit
GTCGTGACTGGGAAAACCCTGGCAAAGCGTCGTGATATTAAATCAACTTG790.10950473365399277No Hit
GTCGTGACTGGGAAAACCCTGGCTCAGAGTCGTGATGAGAAGAGTACTTG780.10811859778495489No Hit
GTCGTGACTGGGAAAACCCTGGGTCATAGTCGTGATGATTTATGCACTTG780.10811859778495489No Hit
GTCGTGACTGGGAAAACCCTGGAGATATGTCGTGATGTAGAGGAGACTTG780.10811859778495489No Hit
GTCGTGACTGGGAAAACCCTGGCGCATGGTCGTGATGGTCAACGAACTTG780.10811859778495489No Hit
GTCGTGACTGGGAAAACCCTGGAGTCCAGTCGTGATGTAGCCGGAACTTG770.10673246191591701No Hit
GTCGTGACTGGGAAAACCCTGGCTCAGAGTCGTGATAAACGAGCCACTTG770.10673246191591701No Hit
GTCGTGACTGGGAAAACCCTGGACGTGTGTCGTGATCCCTATGGAACTTG770.10673246191591701No Hit
GTCGTGACTGGGAAAACCCTGGCCAGTGGTCGTGATGATAACGGGCACTT760.10534632604687912No Hit
GTCGTGACTGGGAAAACCCTGGGTCAAGGTCGTGATCTAAAATGGACTTG760.10534632604687912No Hit
GTCGTGACTGGGAAAACCCTGGGTCACGGTCGTGATCGTGGAGATACTTG760.10534632604687912No Hit
GTCGTGACTGGGAAAACCCTGGCCCGGGGTCGTGACGTATATTCGACTTG750.10396019017784124No Hit
GTCGTGACTGGGAAAACCCTGGATAGCAGTCGTGATGGTATGTGAACTTG740.10257405430880336No Hit
GTCGTGACTGGGAAAACCCTGGTTAGTAGTCGTGATGTGTACTCGACTTG730.10118791843976548No Hit
GTCGTGACTGGGAAAACCCTGGAAAAACGTCGTGATCCATTCACTACTTG730.10118791843976548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGA151.582519E-5242.276412
TCGCGAC1350.0242.276382
GACTGCG100.001526654242.108286
GTGACGG1600.0242.108284
TATCGGG100.001526654242.108286
TGACGGG1500.0242.108265
TGACAGG750.0242.108265
GACGGGG650.0242.108256
TCGTGAC61550.0241.882752
GACTGGG62150.0241.718726
GTGACTG60350.0241.50654
TGGGAAA59000.0239.646159
CGTGGCT2700.0233.14133
CGTGACT62450.0231.446963
ACTGGGA63400.0230.079247
GTCGCGA1450.0225.567691
TGACTGG64950.0224.402115
CTGGGAA65250.0221.14418
GACGGGA1050.0219.050326
TGGGGGA1050.0219.050329
Produced by FastQC (version 0.11.5)