FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868443
Sequences flagged as poor quality0
Sequence length248
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTTGCGGGTCGTGATGTGAGGTTTTAGGC102020.5460161214444326No Hit
GTCGTGACTGGGAAAACCCTGGGATGTGGTCGTGATTGAGCCGCCTTAGG72330.38711376263552055No Hit
GTCGTGACTGGGAAAACCCTGGACCCTAGTCGTGATAGCGGCGCGTTAGG55440.2967176413730577No Hit
GTCGTGACTGGGAAAACCCTGGACCACGGTCGTGATTAATAACTGTTAGG34160.18282602145208604No Hit
GTCGTGACTGGGAAAACCCTGGCCGATAGTCGTGATGCCGAGGGCTTAGG33420.17886550459393194No Hit
GTCGTGACTGGGAAAACCCTGGCATTAAGTCGTGATAGCTTCGCATTAGG19270.1031339998062558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1720300.0241.738362
ACTGGGA1744750.0241.180477
GTGACTG1746200.0240.659564
TGACTGG1744900.0240.584475
TGGGAAA1714450.0239.776269
GACTGGG1768000.0239.327946
CGTGACT1754650.0234.227023
ACGGGGA28300.0229.67887
GTGACGG27050.0222.136414
CTGGGAA1888350.0221.848188
CGTGACG19150.0220.201643
TCGAGAC3800.0220.144822
CGTGACA21700.0220.012123
TGACGGG27100.0218.932925
GACGGGG31300.0218.908846
GTGGTGA55600.0217.271241
CGTGGCT10750.0207.40513
TGACAGG24350.0205.369235
GTGACAG24600.0204.863024
TGGTGAC60950.0188.771072
Produced by FastQC (version 0.11.5)