FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138889
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCCATAGGTCGTGATGATGCGAGTGATCA29980.26323899870839035No Hit
GTCGTGACTGGGAAAACCCTGGCTCAGTGTCGTGACATGAGAGGTGATCA28970.25437070689066277No Hit
GTCGTGACTGGGAAAACCCTGGCACCCCGTCGGATTATACAGTGAGATCA28410.24945363419964545No Hit
GTCGTGACTGGGAAAACCCTGGCTGCTAGTCGTGATCACGTAGAGGATCA26690.23435119664866377No Hit
GTCGTGACTGGGAAAACCCTGGATTTTCGTCGTGATGAATACGACGATCA26340.23127802621677793No Hit
GTCGTGACTGGGAAAACCCTGGCATATCGTCGTGATACGACGAAAGATCA26080.22899509961023418No Hit
GTCGTGACTGGGAAAACCCTGGGCAGTCGTCGTGATGGTCGTCAGGATCA25670.22539509996145368No Hit
GTCGTGACTGGGAAAACCCTGGCCATTCGTCGTGATTTAGATGCTGATCA24990.21942436883664695No Hit
GTCGTGACTGGGAAAACCCTGGGCGGAAGTCGTGATATTGATGGAGATCA24740.21722924709958566No Hit
GTCGTGACTGGGAAAACCCTGGCTAACCGTCGTGATAGCGATGGAGATCA24490.21503412536252436No Hit
GTCGTGACTGGGAAAACCCTGGAACAACGTCGTGATTGATGGAGAGATCA23840.209326808846165No Hit
GTCGTGACTGGGAAAACCCTGGTATGAGGTCGTGATCTAATGATCGATCA23280.2044097361551477No Hit
GTCGTGACTGGGAAAACCCTGGTCACCAGTCGTGATAATGGCAGCGATCA23250.20414632154670034No Hit
GTCGTGACTGGGAAAACCCTGGCGACGAGTCGTGATAGAGATTTTGATCA23210.20379510206877052No Hit
GTCGTGACTGGGAAAACCCTGGCGTCATGTCGTGATCTGCATGAGGATCA23200.20370729719928807No Hit
GTCGTGACTGGGAAAACCCTGGAAGAAAGTCGTGATCGCCTCGGAGATCA22770.19993168781154264No Hit
GTCGTGACTGGGAAAACCCTGGTTGCCTGTCGTGATAAGAATTATGATCA22670.19905363911671814No Hit
GTCGTGACTGGGAAAACCCTGGGAGCATGTCGTGATCGCGAAGGCGATCA21970.1929072982529465No Hit
GTCGTGACTGGGAAAACCCTGGACCTAAGTCGTGATAACCAAGATGATCA21660.1901853472989905No Hit
GTCGTGACTGGGAAAACCCTGGAAGCCTGTCGTGATATATAGTGCGATCA21370.1876390060839994No Hit
GTCGTGACTGGGAAAACCCTGGCCGGTAGTCGTGATACGAGGAGTGATCA21300.18702437199762226No Hit
GTCGTGACTGGGAAAACCCTGGTGAATCGTCGTACTCAGGATGGGGATCA21120.1854438843469381No Hit
GTCGTGACTGGGAAAACCCTGGATTTGCGTCGTGATGGATGCGGGGATCA21110.18535607947745567No Hit
GTCGTGACTGGGAAAACCCTGGCATCCTGTCGTGATGAGCAAGTTGATCA20850.18307315287091191No Hit
GTCGTGACTGGGAAAACCCTGGGGCTTAGTCGTGATGTTATGGAAGATCA20800.18263412852349967No Hit
GTCGTGACTGGGAAAACCCTGGTCATTTGTCGTGATAGCGCTTCCGATCA20380.1789463240052367No Hit
GTCGTGACTGGGAAAACCCTGGAGGCATGTCGTGATCCGAAGGGGGATCA20240.17771705583248237No Hit
GTCGTGACTGGGAAAACCCTGGAAGGTCGTCGTGATACCAAGAGGGATCA20130.1767512022681754No Hit
GTCGTGACTGGGAAAACCCTGGGACGTAGTCGTGATACCGCAAGCGATCA20070.1762243730512807No Hit
GTCGTGACTGGGAAAACCCTGGGGGGCTGTCGTGATCGTACTGCCGATCA20050.17604876331231575No Hit
GTCGTGACTGGGAAAACCCTGGTTGGAGGTCGTGATGGCGAGGGGGATCA19860.1743804707921492No Hit
GTCGTGACTGGGAAAACCCTGGAACAAGGTCGTGATGAAAGGTATGATCA19410.17042925166543885No Hit
GTCGTGACTGGGAAAACCCTGGCATTTCGTCGTGATGGCCCGACTGATCA19400.1703414467959564No Hit
GTCGTGACTGGGAAAACCCTGGTTGACTGTCGTGATCCTATACAAGATCA19160.16823412992837758No Hit
GTCGTGACTGGGAAAACCCTGGGCCAACGTCGTGATGACGCCACGGATCA19000.16682925201665835No Hit
GTCGTGACTGGGAAAACCCTGGGGTGATGTCGTGATATCGAAGGCGATCA18840.1654243741049391No Hit
GTCGTGACTGGGAAAACCCTGGCCACCGGTCGTGATTAAAGGGCAGATCA18760.16472193514907948No Hit
GTCGTGACTGGGAAAACCCTGGCAACTTGTCGTGATTCCAATCAAGATCA18680.16401949619321987No Hit
GTCGTGACTGGGAAAACCCTGGCACCGTCGTGATAGTAGGATACTGATCA18370.16129754523926387No Hit
GTCGTGACTGGGAAAACCCTGGCTGATGGTCGTGATATGCATGACGATCA18220.1599804721970271No Hit
GTCGTGACTGGGAAAACCCTGGCAGCTCGTCGTGATCGAAGAGCCGATCA18060.15857559428530787No Hit
GTCGTGACTGGGAAAACCCTGGGCTAATGTCGTGATTCTAGGACCGATCA18010.15813656993789563No Hit
GTCGTGACTGGGAAAACCCTGGCTGCACGTCGTGATACGCGGTTTGATCA17970.1577853504599658No Hit
GTCGTGACTGGGAAAACCCTGGGCCATGGTCGTGATGAGGTCTACGATCA17890.1570829115041062No Hit
GTCGTGACTGGGAAAACCCTGGCCAATAGTCGTGATGTGCAGAAAGATCA17660.1550633995060098No Hit
GTCGTGACTGGGAAAACCCTGGTAGACTGTCGTGATGGGATGTACGATCA17660.1550633995060098No Hit
GTCGTGACTGGGAAAACCCTGGTTGAGTGTCGTGATACGGATTATGATCA17470.15339510698584322No Hit
GTCGTGACTGGGAAAACCCTGGCCCACTGGTCGTGATTGTACGGAGATCA17440.15313169237739585No Hit
GTCGTGACTGGGAAAACCCTGGTGTATTGTCGTGATAGTGTAGAGGATCA17430.15304388750791342No Hit
GTCGTGACTGGGAAAACCCTGGGAACTGGTCGTGATCGTTAGTTCGATCA17410.1528682777689485No Hit
GTCGTGACTGGGAAAACCCTGGTATAACGTCGTGATTTGCGGACCGATCA17330.1521658388130889No Hit
GTCGTGACTGGGAAAACCCTGGTGAGTTGTCGTGATTACAAGGTTGATCA17310.151990229074124No Hit
GTCGTGACTGGGAAAACCCTGGTCGGACGTCGTGATTCCCGCCAGGATCA17230.15128779011826438No Hit
GTCGTGACTGGGAAAACCCTGGAGCACTGTCGTGATAAAGACTGGGATCA16830.1477755953389663No Hit
GTCGTGACTGGGAAAACCCTGGAGTCCTGTCGTGATTTCAATACAGATCA16820.14768779046948385No Hit
GTCGTGACTGGGAAAACCCTGGTATATTGTCGTGATATACGAGTGGATCA16740.14698535151362424No Hit
GTCGTGACTGGGAAAACCCTGGCCACTTGTCGTGATACGGTGGATGATCA16720.14680974177465933No Hit
GTCGTGACTGGGAAAACCCTGGCGCCTGGTCGTGATAGTTACACTGATCA16580.145580473601905No Hit
GTCGTGACTGGGAAAACCCTGGCCATCCGTCGTGATCTAGATGAAGATCA16570.14549266873242256No Hit
GTCGTGACTGGGAAAACCCTGGGTGTGAGTCGTGATACAAGGCCTGATCA16420.1441755956901858No Hit
GTCGTGACTGGGAAAACCCTGGGCTGAAGTCGTGATAGTTAAGAAGATCA16360.14364876647329108No Hit
GTCGTGACTGGGAAAACCCTGGAAAACAGTCGTGATGGGACCACTGATCA16360.14364876647329108No Hit
GTCGTGACTGGGAAAACCCTGGATCCGCGTCGTGATAATTAGGCTGATCA16320.14329754699536126No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGGGTCGTGATTCAAATGAAGATCA16310.1432097421258788No Hit
GTCGTGACTGGGAAAACCCTGGATTCGGGTCGTGATTGTGAGGAAGATCA16230.1425073031700192No Hit
GTCGTGACTGGGAAAACCCTGGAAGGCTGTCGTGATCACGGTGATGATCA16200.14224388856157183No Hit
GTCGTGACTGGGAAAACCCTGGCTCAAGGTCGTGATAAAACACCCGATCA16180.14206827882260695No Hit
GTCGTGACTGGGAAAACCCTGGGAGGGTGTCGTGATAATACGAAAGATCA16170.1419804739531245No Hit
GTCGTGACTGGGAAAACCCTGGCCCGTCGTCGTGATATAATTGATGATCA16170.1419804739531245No Hit
GTCGTGACTGGGAAAACCCTGGAAGGTAGTCGTGATCTCACGAACGATCA16160.14189266908364204No Hit
GTCGTGACTGGGAAAACCCTGGTACTAGGTCGTGATTGGGAGGTGGATCA16150.14180486421415958No Hit
GTCGTGACTGGGAAAACCCTGGTTTGAAGTCGTGATCTCAAGGAGGATCA16090.14127803499726488No Hit
GTCGTGACTGGGAAAACCCTGGGAGAGCGTCGTGATCTGGACTGCGATCA15990.14039998630244035No Hit
GTCGTGACTGGGAAAACCCTGGTGCCTTGTCGTGATCAGGACCCTGATCA15990.14039998630244035No Hit
GTCGTGACTGGGAAAACCCTGGGGATTAGTCGTGATGTGAACGAAGATCA15900.1396097424770983No Hit
GTCGTGACTGGGAAAACCCTGGCCACGCGTCGTGATAGGAACAAGGATCA15870.13934632786865095No Hit
GTCGTGACTGGGAAAACCCTGGCCGATGGTCGTGATCATATAAGAGATCA15630.1372390110010721No Hit
GTCGTGACTGGGAAAACCCTGGGGCAAGGTCGTGATCCGGATTCCGATCA15460.13574632821987043No Hit
GTCGTGACTGGGAAAACCCTGGAACAACGTCGTGATGGTCTGTAGGATCA15440.13557071848090552No Hit
GTCGTGACTGGGAAAACCCTGGCAGAGCGTCGTGATTAATCACTTGATCA15420.1353951087419406No Hit
GTCGTGACTGGGAAAACCCTGGTTGCGCGTCGTGATCTGGTCATCGATCA15390.13513169413349324No Hit
GTCGTGACTGGGAAAACCCTGGACCATTGTCGTGATACCATGATGGATCA15110.1326731577879846No Hit
GTCGTGACTGGGAAAACCCTGGGCCTTAGTCGTGATGTCTATCCCGATCA15110.1326731577879846No Hit
GTCGTGACTGGGAAAACCCTGGTGGTGTGTCGTGATGGGTACCTCGATCA15100.13258535291850215No Hit
GTCGTGACTGGGAAAACCCTGGTTGCTAGTCGTGATTCGACGGGGGATCA15090.1324975480490197No Hit
GTCGTGACTGGGAAAACCCTGGCTTGCAGTCGTGATTCTAACACGGATCA14980.13153169448471275No Hit
GTCGTGACTGGGAAAACCCTGGCCGATGGTCGTGATCTAAAGGGCGATCA14910.13091706039833556No Hit
GTCGTGACTGGGAAAACCCTGGGCGGCCGTCGTGATGGATATCCAGATCA14900.13082925552885313No Hit
GTCGTGACTGGGAAAACCCTGGCATAACGTCGTGATCGTATCGACGATCA14880.1306536457898882No Hit
GTCGTGACTGGGAAAACCCTGGCCCCCTGTCGTGATCGTTACACTGATCA14810.13003901170351106No Hit
GTCGTGACTGGGAAAACCCTGGCCCGAGGTCGTGATGTTAACAGCGATCA14800.1299512068340286No Hit
GTCGTGACTGGGAAAACCCTGGGACCAGGTCGTGATCGTAAGATCGATCA14780.1297755970950637No Hit
GTCGTGACTGGGAAAACCCTGGTCATTCGTCGTGAGCCTGCCCTTGATCA14760.1295999873560988No Hit
GTCGTGACTGGGAAAACCCTGGACCGAGGTCGTGATCAAGTGACTGATCA14590.1281073045748971No Hit
GTCGTGACTGGGAAAACCCTGGGTAATCGTCGTGATGTGAACAACGATCA14440.12679023153266034No Hit
GTCGTGACTGGGAAAACCCTGGTAAGTAGTCGTGATAGTGGCATTGATCA14320.1257365730988709No Hit
GTCGTGACTGGGAAAACCCTGGTACCATGTCGTGATTAAGAGTCTGATCA14210.12477071953456394No Hit
GTCGTGACTGGGAAAACCCTGGGGTCCCGTCGTGATATCGCACAGGATCA13990.12283901240595001No Hit
GTCGTGACTGGGAAAACCCTGGCCCCAGGTCGTGATTCCATAGGGGATCA13930.12231218318905529No Hit
GTCGTGACTGGGAAAACCCTGGCGCAGTGTCGTGATCCAGGCGTCGATCA13840.12152193936371322No Hit
GTCGTGACTGGGAAAACCCTGGCGGAGTGTCGTGATTCTAAGAGTGATCA13680.12011706145199401No Hit
GTCGTGACTGGGAAAACCCTGGCGGGACGTCGTGATTTCCGGGCTGATCA13610.11950242736561685No Hit
GTCGTGACTGGGAAAACCCTGGACTGATGTCGTGATTTACAAGATGATCA13520.11871218354027478No Hit
GTCGTGACTGGGAAAACCCTGGTCTCGGGTCGTGATTCCCTCGGAGATCA13520.11871218354027478No Hit
GTCGTGACTGGGAAAACCCTGGGGGGCTGTCGTGATAATAAAGGCGATCA13400.11765852510648535No Hit
GTCGTGACTGGGAAAACCCTGGCACTTCGTCGTGATCGTAACGAAGATCA13350.1172195007590731No Hit
GTCGTGACTGGGAAAACCCTGGAAGACAGTCGTGATCCCGGAGGAGATCA13300.11678047641166085No Hit
GTCGTGACTGGGAAAACCCTGGGGCTATGTCGTGATCATATTAGCGATCA13290.11669267154217838No Hit
GTCGTGACTGGGAAAACCCTGGCGCACAGTCGTGATAGGATGGACGATCA13250.11634145206424858No Hit
GTCGTGACTGGGAAAACCCTGGCCCTAGGTCGTGATGAGTGTGCTGATCA13210.11599023258631877No Hit
GTCGTGACTGGGAAAACCCTGGGTACGAGTCGTGATAATAGGTTCGATCA13130.11528779363045917No Hit
GTCGTGACTGGGAAAACCCTGGAGGCTAGTCGTGATGCAACGTTGGATCA13100.1150243790220118No Hit
GTCGTGACTGGGAAAACCCTGGCTCATTGTCGTGATGTGCGTATGGATCA13050.11458535467459956No Hit
GTCGTGACTGGGAAAACCCTGGTTACACGTCGTGATCAAAACCGTGATCA13000.11414633032718728No Hit
GTCGTGACTGGGAAAACCCTGGATCCCTGTCGTGAATGACCCCCTGATCA12990.11405852545770484No Hit
GTCGTGACTGGGAAAACCCTGGGCCCTGGTCGTGATGTGACCCCCGATCA12980.11397072058822239No Hit
GTCGTGACTGGGAAAACCCTGGCAACCTGTCGTGATGTGGTATGAGATCA12960.11379511084925749No Hit
GTCGTGACTGGGAAAACCCTGGCCATATGTCGTGATTTTGCTGCTGATCA12860.11291706215443295No Hit
GTCGTGACTGGGAAAACCCTGGAGCTCTGTCGTGATGTATCGAAAGATCA12810.1124780378070207No Hit
GTCGTGACTGGGAAAACCCTGGAATAATGTCGTGATACAGATGTGGATCA12750.11195120859012599No Hit
GTCGTGACTGGGAAAACCCTGGACCACAGTCGTGATATATACGAAGATCA12670.11124876963426637No Hit
GTCGTGACTGGGAAAACCCTGGGGTGTGGTCGTGATAGGTACCAGGATCA12570.11037072093944186No Hit
GTCGTGACTGGGAAAACCCTGGAAGAGCGTCGTGATCTCTACACTGATCA12540.11010730633099451No Hit
GTCGTGACTGGGAAAACCCTGGTAGGCAGTCGTGATCCCGAATTGGATCA12530.11001950146151206No Hit
GTCGTGACTGGGAAAACCCTGGCCAAGGGTCGTGATAGGCTGTGAGATCA12420.10905364789720509No Hit
GTCGTGACTGGGAAAACCCTGGACGCAGGTCGTGATGTATAGAACGATCA12410.10896584302772262No Hit
GTCGTGACTGGGAAAACCCTGGGGGCAGGTCGTGATTGCGGCCATGATCA12400.10887803815824018No Hit
GTCGTGACTGGGAAAACCCTGGAGCAAGGTCGTGATTCCGAGAGCTCTCA12360.10852681868031037No Hit
GTCGTGACTGGGAAAACCCTGGTTGGACGTCGTGATTATAAAAAGGATCA12270.1077365748549683No Hit
GTCGTGACTGGGAAAACCCTGGAAAAGCGTCGTGATATCATTACTGATCA12240.10747316024652094No Hit
GTCGTGACTGGGAAAACCCTGGGTTGACGTCGTTCTGGAGGACAGGATCA12230.1073853553770385No Hit
GTCGTGACTGGGAAAACCCTGGCGCGGTGTCGTGATATGACTAGCGATCA12150.1066829164211789No Hit
GTCGTGACTGGGAAAACCCTGGGCTTGGGTCGTGATTGCGAGTGCGATCA12050.10580486772635436No Hit
GTCGTGACTGGGAAAACCCTGGGCAGAAGTCGTGATTAATACGGCGATCA12030.10562925798738947No Hit
GTCGTGACTGGGAAAACCCTGGGCAGTTGTCGTGATGAATAGGAGGATCA11970.10510242877049475No Hit
GTCGTGACTGGGAAAACCCTGGGTTGGGGTCGTGATGGAAAGGACGATCA11950.10492681903152985No Hit
GTCGTGACTGGGAAAACCCTGGAACTCAGTCGTGATCCTCGGTTTGATCA11930.10475120929256494No Hit
GTCGTGACTGGGAAAACCCTGGGTTCCCGTCGTGATCGTGAGGATGATCA11920.1046634044230825No Hit
GTCGTGACTGGGAAAACCCTGGGCAGTTGTCGTGATGATTCCTTTGATCA11920.1046634044230825No Hit
GTCGTGACTGGGAAAACCCTGGCGACTTGTCGTGATATGCGGATTGATCA11870.10422438007567025No Hit
GTCGTGACTGGGAAAACCCTGGCAGTCGGTCTGATGACCGGGGGCGATCA11850.10404877033670533No Hit
GTCGTGACTGGGAAAACCCTGGCCAGTCGTCGTGATATGTAGATGGATCA11760.10325852651136326No Hit
GTCGTGACTGGGAAAACCCTGGTGTGCTGTCGTGATAAGTGGGACGATCA11730.1029951119029159No Hit
GTCGTGACTGGGAAAACCCTGGTAAAATGTCGTGATTTCCTGAAAGATCA11680.10255608755550365No Hit
GTCGTGACTGGGAAAACCCTGGGAACCTGTCGTGATCACGTGGCGGATCA11660.10238047781653875No Hit
GTCGTGACTGGGAAAACCCTGGCTTGAAGTCGTGATTATCTAGATGATCA11620.10202925833860893No Hit
GTCGTGACTGGGAAAACCCTGGCGAACCGTCGTGATAGTTATGAAGATCA11560.10150242912171424No Hit
GTCGTGACTGGGAAAACCCTGGCCGACAGTCGTGATTTGTTCGACGATCA11510.10106340477430197No Hit
GTCGTGACTGGGAAAACCCTGGGACGAAGTCGTGATTCGGAGTAGGATCA11510.10106340477430197No Hit
GTCGTGACTGGGAAAACCCTGGCCTCCAGTCGTGATAACGTATTAGATCA11500.10097559990481952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)