FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494886
Sequences flagged as poor quality0
Sequence length248
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA1427600.0240.738857
TCGTGAC1439800.0240.458012
TGGGAAA1403800.0239.398189
GTGACTG1466700.0236.016534
TGACTGG1467400.0235.781975
CGTGACT1471800.0234.831563
GACTGGG1472150.0234.726946
CTGGGAA1488550.0231.703238
CGTGGCT22250.0206.905643
TGGGAAC33700.0177.057429
CGTTACA750.0161.53163
TGCGAAA900.0161.374859
CGTGACA4600.0158.020033
TCGAGAC1700.0156.854222
GTGACTT8250.0149.713644
GTCGTGA2789850.0124.150891
TCGTGGC32650.0121.019912
GCGTGGC8350.0120.479752
CGTTACT2000.0115.0912553
GTGACAG6650.0114.718664
Produced by FastQC (version 0.11.5)