FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096821
Sequences flagged as poor quality0
Sequence length248
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGACACAAGTCGTGATTCCCTGTCCCAGTG152261.3881936979689484No Hit
GTCGTGACTGGGAAAACCCTGGCCGAAGGTCGTGATTAAACCGTGCAGTG138101.2590933251642702No Hit
GTCGTGACTGGGAAAACCCTGGCATTTGGTCGTGATCTATACGGACAGTG128271.1694706793542429No Hit
GTCGTGACTGGGAAAACCCTGGCAATGTGTCGTGATCGGTATATCCAGTG110431.0068187972330946No Hit
GTCGTGACTGGGAAAACCCTGGGACCCAGTCGTGATATAGAGGTTCAGTG99430.9065289596023417No Hit
GTCGTGACTGGGAAAACCCTGGTGCGGTGTCGTGATAGCCGGCCTCAGTG96790.882459398570961No Hit
GTCGTGACTGGGAAAACCCTGGAATCAGTCGTGATACGTCTACTTCAGTG96630.8810006372963318No Hit
GTCGTGACTGGGAAAACCCTGGAATCAGTCCTGATACGTCTACTTCAGTG94160.8584810101192447No Hit
GTCGTGACTGGGAAAACCCTGGATAGACGTCGTGATAAGCTCCATCAGTG93790.8551076246716648No Hit
GTCGTGACTGGGAAAACCCTGGTAACCGGTCGTGATAGGGGAGTTCAGTG92940.8473579554001974No Hit
GTCGTGACTGGGAAAACCCTGGACGGGTGTCGTGATCTTATTAAACAGTG87240.7953895849915346No Hit
GTCGTGACTGGGAAAACCCTGGGCTGAAGTCGTGATCTAAACCCGCAGTG84830.7734169932924333No Hit
GTCGTGACTGGGAAAACCCTGGCGCTCAGTCGTGATTACATCGGTCAGTG83550.7617469030954003No Hit
GTCGTGACTGGGAAAACCCTGGTGCAGGGTCGTGATGCAGGCAGACAGTG83390.7602881418207711No Hit
GTCGTGACTGGGAAAACCCTGGTTAACGGTCGTGATATTTGCCCGCAGTG80790.7365832711080477No Hit
GTCGTGACTGGGAAAACCCTGGGCCACCGTCGTGATGTACTCCAGCAGTG79280.7228162115787352No Hit
GTCGTGACTGGGAAAACCCTGGTTACCCGTCGTGATACTTTCCCGCAGTG76100.6938233312454812No Hit
GTCGTGACTGGGAAAACCCTGGCCTTTCGTCGTGATATTTAGAGCCAGTG75220.685800144235021No Hit
GTCGTGACTGGGAAAACCCTGGTAGGCTGTCGTGATCCAAATCCGCAGTG74610.6802386168754975No Hit
GTCGTGACTGGGAAAACCCTGGGATCAGGTCGTGATTAGCTTTGTCAGTG74500.67923571849919No Hit
GTCGTGACTGGGAAAACCCTGGAGAACCGTCGTGATATTTTATACCAGTG71990.6563514010034455No Hit
GTCGTGACTGGGAAAACCCTGGGCCCGTGTCGTGATGGGAGTATTCAGTG71470.6516104268609008No Hit
GTCGTGACTGGGAAAACCCTGGGAGAGTGTCGTGATAGGAACATTCAGTG71270.6497869752676143No Hit
GTCGTGACTGGGAAAACCCTGGACAGGTGTCGTGATGTCAATATGCAGTG71110.6483282139929851No Hit
GTCGTGACTGGGAAAACCCTGGCGCAAGGTCGTGATGATGATATTCAGTG70190.6399403366638676No Hit
GTCGTGACTGGGAAAACCCTGGTGCGAAGTCGTGATCAACCCTACCAGTG69000.6290907996838134No Hit
GTCGTGACTGGGAAAACCCTGGAATATCGTCGTGATATAAACTATCAGTG67280.6134091159815503No Hit
GTCGTGACTGGGAAAACCCTGGTCTGTGGTCGTGATAAATGTTTTCAGTG67240.6130444256628931No Hit
GTCGTGACTGGGAAAACCCTGGATTATGGTCGTGATTCATTAGACCAGTG66990.6107651111712851No Hit
GTCGTGACTGGGAAAACCCTGGGAGACAGTCGTGATAGCAAGAAGCAGTG66210.603653649957468No Hit
GTCGTGACTGGGAAAACCCTGGTGTCGTGTCGTGATGATCACGATCAGTG63700.5807693324617235No Hit
GTCGTGACTGGGAAAACCCTGGCAAACGGTCGTGATGTAGCCCGACAGTG63480.5787635357091084No Hit
GTCGTGACTGGGAAAACCCTGGGCTGAAGTCGTGATAGGTAGGGGCAGTG62250.567549308410397No Hit
GTCGTGACTGGGAAAACCCTGGTACAAAGTCGTGATCCCAAGTCGCAGTG62140.5665464100340893No Hit
GTCGTGACTGGGAAAACCCTGGGTCTGCGTCGTGATATACATCCGCAGTG61650.5620789536305377No Hit
GTCGTGACTGGGAAAACCCTGGATTCCCGTCGTGATGGATGACCGCAGTG59870.5458502344502886No Hit
GTCGTGACTGGGAAAACCCTGGCCATGGGTCGTGATGACTGGTGTCAGTG59440.5419298135247228No Hit
GTCGTGACTGGGAAAACCCTGGCAGGGTGTCGTGATTCTCGGTTTCAGTG56540.5154897654220698No Hit
GTCGTGACTGGGAAAACCCTGGGATATGGTCGTGATGCTACGATTCAGTG54910.5006286349367854No Hit
GTCGTGACTGGGAAAACCCTGGAACTCAGTCGTGATAACTATTTTCAGTG54480.49670821401121973No Hit
GTCGTGACTGGGAAAACCCTGGCTCATTGTCGTGATGGCAACACACAGTG52830.4816647383666068No Hit
GTCGTGACTGGGAAAACCCTGGTGGAGCGTCGTGATACAGCTCTGCAGTG52570.47929425129533443No Hit
GTCGTGACTGGGAAAACCCTGGCTCCATGTCGTGATATAGTGAAACAGTG52400.477744317441041No Hit
GTCGTGACTGGGAAAACCCTGGCTGCAGGTCGTGATGCCAAGAGACAGTG52370.47747079970204803No Hit
GTCGTGACTGGGAAAACCCTGGGAATCTGTCGTGATGTTATCCCACAGTG51640.4708152013865526No Hit
GTCGTGACTGGGAAAACCCTGGATCTTAGTCGTGATCCTTAATATCAGTG51640.4708152013865526No Hit
GTCGTGACTGGGAAAACCCTGGAGCCACGTCGTGACTGGCCATTTCAGTG51560.47008582074923805No Hit
GTCGTGACTGGGAAAACCCTGGAACGAGGTCGTGATGAGGACCCGCAGTG51330.4679888514169586No Hit
GTCGTGACTGGGAAAACCCTGGTTTAATGTCGTGATGGTCAAGAGCAGTG51230.4670771256203155No Hit
GTCGTGACTGGGAAAACCCTGGCAGATAGTCGTGATATACGGAAGCAGTG50870.46379491275239987No Hit
GTCGTGACTGGGAAAACCCTGGCATCGCGTCGTGATGCTAAAGGCCAGTG50380.4593274563488482No Hit
GTCGTGACTGGGAAAACCCTGGAAAAACGTCGTGATGCGGAAGAGCAGTG48650.4435546000669207No Hit
GTCGTGACTGGGAAAACCCTGGCCGTGAGTCGTGATTATATACCCCAGTG48500.4421870113719559No Hit
GTCGTGACTGGGAAAACCCTGGCAAACAGTCGTGATGCAGAGTGACAGTG47630.43425499694116No Hit
GTCGTGACTGGGAAAACCCTGGTGCCTTGTCGTGATGTCATGACTCAGTG47530.43334327114451676No Hit
GTCGTGACTGGGAAAACCCTGGCACGGCGTCGGATCGTGCCCGTGCAGTG47510.43316092598518807No Hit
GTCGTGACTGGGAAAACCCTGGGACTCAGTCGTGATTAATGATCTCAGTG47130.42969636795794386No Hit
GTCGTGACTGGGAAAACCCTGGATCGCCGTCGTGATGAAGATTCCCAGTG46070.420032074513526No Hit
GTCGTGACTGGGAAAACCCTGGATAGAAGTCGTGATAGTGAGGGGCAGTG45110.4112795068657511No Hit
GTCGTGACTGGGAAAACCCTGGATTCCTGTCGTGATGGCGAGATGCAGTG45040.41064129880810085No Hit
GTCGTGACTGGGAAAACCCTGGGCACCCGTCGTGATGTGATGCTGCAGTG44080.4018887311603261No Hit
GTCGTGACTGGGAAAACCCTGGAAAGATGTCGTGATTCGGGGGTGCAGTG44000.40115935052301144No Hit
GTCGTGACTGGGAAAACCCTGGGTCTCAGATTCTGTACACTGGTACAGTG43830.399609416668718No Hit
GTCGTGACTGGGAAAACCCTGGATGATCGTCGTGATAATGACATTCAGTG43780.39915355377039646No Hit
GTCGTGACTGGGAAAACCCTGGCGGACAGTCTGATAACAGCTGACCAGTG43640.39787713765509597No Hit
GTCGTGACTGGGAAAACCCTGGGCTAATGTCGTGATGGGTACGTCCAGTG42910.3912215393396005No Hit
GTCGTGACTGGGAAAACCCTGGACCCTGGTCGTGATGATGTATGCCAGTG42880.3909480216006076No Hit
GTCGTGACTGGGAAAACCCTGGAAGAATGTCGTGATAAGGACCCGCAGTG42330.3859335297190699No Hit
GTCGTGACTGGGAAAACCCTGGCCCACCGTCGTGATATGTGGGAGCAGTG42270.385386494241084No Hit
GTCGTGACTGGGAAAACCCTGGGTCCGTGTCGTGATCTGTACCCCCAGTG42170.38447476844444084No Hit
GTCGTGACTGGGAAAACCCTGGAGTAACGTCGTGATCTTCATGGCCAGTG42050.3833806974884689No Hit
GTCGTGACTGGGAAAACCCTGGCCTTCTGTCGTGATATTGAAAGTCAGTG40900.37289585082707205No Hit
GTCGTGACTGGGAAAACCCTGGCCGTTGGTCGTGATTGCGAGAAGCAGTG40560.36979598311848516No Hit
GTCGTGACTGGGAAAACCCTGGCATCATGTCGTGATGGGCTCCCGCAGTG40380.36815487668452734No Hit
GTCGTGACTGGGAAAACCCTGGTCTCGAGTCGTGATAACCTGGGGCAGTG40090.36551087187426207No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGGGTCGTGATTCAAACACCCAGTG39870.363505075121647No Hit
GTCGTGACTGGGAAAACCCTGGAAGACCGTCGTGATCTTATTAAACAGTG39830.3631403848029897No Hit
GTCGTGACTGGGAAAACCCTGGCTACCGGTCGTGATAATTCGTGGCAGTG39750.3624110041656752No Hit
GTCGTGACTGGGAAAACCCTGGCCTATGGTCGTGATTGCTGGAACCAGTG39300.3583082380807807No Hit
GTCGTGACTGGGAAAACCCTGGGATAAAGTCGTGATAACATGGATCAGTG39030.3558465784298441No Hit
GTCGTGACTGGGAAAACCCTGGCCGCTGGTCGTGATCGCGTCGCCCAGTG38910.3547525074738722No Hit
GTCGTGACTGGGAAAACCCTGGAGTTAGGTCGTGATAACCGGTGGCAGTG38750.3532937461992431No Hit
GTCGTGACTGGGAAAACCCTGGCTTAAAGTCGTGATCATCCCTGCCAGTG38710.3529290558805858No Hit
GTCGTGACTGGGAAAACCCTGGGTAGCTGTCGTGATTCTTAGTACCAGTG38600.35192615750427825No Hit
GTCGTGACTGGGAAAACCCTGGGGAGGAGTCGTGATGGTAATATTCAGTG38260.34882628979569136No Hit
GTCGTGACTGGGAAAACCCTGGCAGAGTGTCGTGATTGGAAACCTCAGTG37290.33998254956825225No Hit
GTCGTGACTGGGAAAACCCTGGATTCAAGTCGTGATCGCAAGGGTCAGTG36830.3357886109036935No Hit
GTCGTGACTGGGAAAACCCTGGGATCTTGTCGTGATACTTACCGTCAGTG36800.3355150931647005No Hit
GTCGTGACTGGGAAAACCCTGGACGTAGGTCGTGATTGGGAGCCTCAGTG36350.3314123270798061No Hit
GTCGTGACTGGGAAAACCCTGGCGTCCCGTCGTGATAGTCGAGCCCAGTG36100.3291330125881981No Hit
GTCGTGACTGGGAAAACCCTGGTTGGGTGTCGTGATAAGCTACCGCAGTG35330.3221127239540454No Hit
GTCGTGACTGGGAAAACCCTGGCCTAACGTCGTGATATGCGATGGCAGTG34940.31855699334713683No Hit
GTCGTGACTGGGAAAACCCTGGGTGCCGGTCGTGATACTCACCGTCAGTG34300.3127219482486203No Hit
GTCGTGACTGGGAAAACCCTGGCAAGTGGTCGTGATAACAAGGTGCAGTG34000.3099867708586907No Hit
GTCGTGACTGGGAAAACCCTGGCTCTTTGTCGTGATCACAAGGCCCAGTG33810.3082544918450686No Hit
GTCGTGACTGGGAAAACCCTGGTTGATGGTCGTGATTTTGCAGATCAGTG33110.3018724112685662No Hit
GTCGTGACTGGGAAAACCCTGGCACTCCGTCGTGATTCAAAGGGACAGTG33010.30096068547192295No Hit
GTCGTGACTGGGAAAACCCTGGTACGGAGTCGTGATCCACATGTCCAGTG32370.29512564037340644No Hit
GTCGTGACTGGGAAAACCCTGGTGAGCTGTCGTGATCTGAATGGCCAGTG32220.2937580516784416No Hit
GTCGTGACTGGGAAAACCCTGGCCGTCGGTCGTGATTACAGACAGCAGTG31560.2877406614205964No Hit
GTCGTGACTGGGAAAACCCTGGCCTTCCGTCGTGATACCAAGTAACAGTG30710.2799909921491292No Hit
GTCGTGACTGGGAAAACCCTGGAAACCGGTCGTGATAAGTCCGGGCAGTG30430.2774381599185282No Hit
GTCGTGACTGGGAAAACCCTGGATGGATGTCGTGATCAACCCTACCAGTG30400.27716464217953524No Hit
GTCGTGACTGGGAAAACCCTGGCTGGTGGTCGTGATCCCTGTGAACAGTG30270.27597939864389903No Hit
GTCGTGACTGGGAAAACCCTGGCCGGTCGTCGTGATGATGGTATACAGTG29490.268867937430082No Hit
GTCGTGACTGGGAAAACCCTGGGCCTATGTCGTGATCCTGGCTCCCAGTG28850.26303289233156546No Hit
GTCGTGACTGGGAAAACCCTGGTTTGGTGTCGTGATCTAATGTCGCAGTG28530.26011536978230726No Hit
GTCGTGACTGGGAAAACCCTGGGCTTGTGTCGTGATGATCTGTATCAGTG28170.2568331569143917No Hit
GTCGTGACTGGGAAAACCCTGGTTTTTTGTCGTGATCGAAGGTACCAGTG28000.25528322306009826No Hit
GTCGTGACTGGGAAAACCCTGGCGCCCTGTCGTGATACTATTCCCCAGTG27990.2551920504804339No Hit
GTCGTGACTGGGAAAACCCTGGGTGAGCGTCGTGATTAGACATGACAGTG27760.25309508114815454No Hit
GTCGTGACTGGGAAAACCCTGGGGTAATGTCGTGATCTTAAGGATCAGTG27680.25236570051083995No Hit
GTCGTGACTGGGAAAACCCTGGGAGAGTGTCGTGATGTTGAGTCCCAGTG27360.2494481779615817No Hit
GTCGTGACTGGGAAAACCCTGGCTCAAAGTCGTGATATTTGAGTTCAGTG26110.2380516055035416No Hit
GTCGTGACTGGGAAAACCCTGGTAGGGGGTCGTGATCACCCGCCTCAGTG26090.23786926034421296No Hit
GTCGTGACTGGGAAAACCCTGGGACTGGGTCGTGATAAGGATCCACAGTG25410.23166952492703913No Hit
GTCGTGACTGGGAAAACCCTGGGGAAGAGTCGTGATGGAAACGAGCAGTG25390.2314871797677105No Hit
GTCGTGACTGGGAAAACCCTGGCGTAGGGTCGTGATGCAAGAAGCCAGTG25240.23011959107274568No Hit
GTCGTGACTGGGAAAACCCTGGTTACAGGTCGTGATGGAAACCCGCAGTG25040.2282961394794593No Hit
GTCGTGACTGGGAAAACCCTGGTTTCACGTCGTGATGTTATAGGGCAGTG24970.22765793142180904No Hit
GTCGTGACTGGGAAAACCCTGGCCCACTGTCGTGATAGAAAGAGCCAGTG24580.2241022008149005No Hit
GTCGTGACTGGGAAAACCCTGGCAAGTGGTCGTGATGATGTTTGTCAGTG24360.22209640406228548No Hit
GTCGTGACTGGGAAAACCCTGGCTTCTTGTCGTGATTGGATGTCACAGTG24180.22045529762832766No Hit
GTCGTGACTGGGAAAACCCTGGACGATGGTCGTGATTGCAATTGTCAGTG23560.2148025976891398No Hit
GTCGTGACTGGGAAAACCCTGGTGCGTAGTCGTGATGTAGAAGTCCAGTG23530.21452907995014683No Hit
GTCGTGACTGGGAAAACCCTGGTGAGTAGTCGTGATGTGAAAACCCAGTG23320.2126144557771961No Hit
GTCGTGACTGGGAAAACCCTGGCAGGGCGTCGTGACAAGAGTGCCCAGTG23120.2107910041839097No Hit
GTCGTGACTGGGAAAACCCTGGAACTGGGTCGTGATGAGCCAGTACAGTG22980.20951458806860918No Hit
GTCGTGACTGGGAAAACCCTGGTCAGGAGTCGTGATTAATCGTATCAGTG22940.2091498977499519No Hit
GTCGTGACTGGGAAAACCCTGGGCACCCGTCGTGATTTACTCCCTCAGTG22770.20759996389565846No Hit
GTCGTGACTGGGAAAACCCTGGGCATATGTCGTGATCTTTTGGAACAGTG22660.20659706551935092No Hit
GTCGTGACTGGGAAAACCCTGGCTCATAGTCGTGATTAAACCCGTCAGTG22530.20541182198371474No Hit
GTCGTGACTGGGAAAACCCTGGGGACTAGTCGTGATTAGTACGTGCAGTG22430.20450009618707155No Hit
GTCGTGACTGGGAAAACCCTGGAGTCGGGTCGTGATCAACAGGGACAGTG22330.20358837039042835No Hit
GTCGTGACTGGAAAAACCCTGGCCCGCTGTCGTGATTTCAGTTTCCAGTG21900.19966794946486255No Hit
GTCGTGACTGGGAAAACCCTGGTCCTTGGTCGTGATAATAGGTCTCAGTG21870.1993944317258696No Hit
GTCGTGACTGGGAAAACCCTGGCTGGATGTCGTGATAATGAGAACCAGTG21460.19565635595963243No Hit
GTCGTGACTGGGAAAACCCTGGTCTCAAGTCGTGATGTCTTCTCTCAGTG21120.19255648825104552No Hit
GTCGTGACTGGGAAAACCCTGGTGCATCGTCGTGATGCTTAATACCAGTG20620.18799785926782947No Hit
GTCGTGACTGGGAAAACCCTGGACTTCAGTCGTGATGTCGTGGGTCAGTG20590.18772434152883652No Hit
GTCGTGACTGGGAAAACCCTGGACTGGGGTCGTGATACCAATTTTCAGTG20360.18562737219655714No Hit
GTCGTGACTGGGAAAACCCTGGGGTGTTGTCGTGATATGTACCAGCAGTG19610.1787894287217331No Hit
GTCGTGACTGGGAAAACCCTGACATCCGTCGTGATAGCATTGGGCCAGTG18630.16985451591462966No Hit
GTCGTGACTGGGAAAACCCTGGGTCGCAGTCGTGATACACTGGCTCAGTG18390.16766637400268594No Hit
GTCGTGACTGGGAAAACCCTGGGAGAAGGTCGTGATGGGTGATACCAGTG17720.16155781116517645No Hit
GTCGTGACTGGGAAAACCCTGGACTCTTGTTTATCAGGATCCACCCAGTG17530.15982553215155437No Hit
GTCGTGACTGGGAAAACCCTGGTTAGGGTGATTTTGGCCATGGCTCAGTG17530.15982553215155437No Hit
GTCGTGACTGGGAAAACCCTGGTTGTGAGTCGTGATGGATACGCACAGTG17480.15936966925323276No Hit
GTCGTGACTGGGAAAACCCTGGGAAACTGTCGTGATCTTTCATTTCAGTG17130.15617862896498153No Hit
GTCGTGACTGGGAAAACCCTGGTGTAGTGTCGTGATGAGATTTCTCAGTG16540.1507994467647866No Hit
GTCGTGACTGGGAAAACCCTGGTTGAACGTCGTGATGCGTAGAGACAGTG16440.1498877209681434No Hit
GTCGTGACTGGGAAAACCCTGGTATTGCGTCGTGATGGCAGGGGACAGTG16260.1482466145341856No Hit
GTCGTGACTGGGAAAACCCTGGTAAAACGTCGTGATCCCCCGCCCCAGTG16260.1482466145341856No Hit
GTCGTGACTGGGAAAACCCTGGGACCCAGTCGTGATTCTAACCCCCAGTG16030.14614964520190624No Hit
GTCGTGACTGGGAAAACCCTGGGAGTTTGTCGTGATGCTGTCTAACAGTG14650.13356782920822996No Hit
GTCGTGACTGGGAAAACCCTGGCGAACAGTCGTGATTAACTATACCAGTG14390.13119734213695763No Hit
GTCGTGACTGGGAAAACCCTGACATCCGTCCTGATAGCATTGGGCCAGTG14310.13046796149964304No Hit
GTCGTGACTGGGGAAATCCCTGGTCCTCGGTCGTGATCTGTCCAGCAGTG14270.13010327118098577No Hit
GTCGTGACTGGGAAAACCCTGGACTTACGGCGGTGACCCAGCTTGCAGTG14000.12764161153004913No Hit
GTCGTGACTGGGAAAACCCTGGCTGTCCGTCGTGATTACAGCCCACAGTG13800.1258181599367627No Hit
GTCGTGACTGGGAAAACCCTGGTATCAAGTCGTGATCAAAAACCTCAGTG13520.12326532770616172No Hit
GTCGTGACTGGGAAAACCCTGGCCCGCTGTCGTGATTTCAGTTTCCAGTG12920.11779497292630248No Hit
GTCGTGACTGGGAAAACCCTGGCTGAATGTCGTGATGTGTCTTCACAGTG12680.11560683101435879No Hit
GTCGTGACTGGGAAAACCCTGGTCCAGAGTCGTGATTGCTAGTAGCAGTG12360.1126893084651005No Hit
GTCGTGACTGGGAAAACCCTGGGTCCTAGTCGTGATAAACCCGGGCAGTG11060.1008368731087388No Hit
GTCGTGACTGGGAAAACCCTGGCGCGGGGTCGTGATAAGTAATCTCAGTG11010.1003810102104172No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)