FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421793
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTAGAGTGTCGTGATCAGCCGGCAGCCAA41580.2924476347822785No Hit
GTCGTGACTGGGAAAACCCTGGCATGCAGTCGTGATTCTCACCCCGCCAA18410.1294843904844095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1295950.0241.715413
TCGTGAC1318350.0241.560152
GTGACTG1327050.0241.4514
TGACTGG1327400.0241.219675
GACTGGG1342450.0240.320886
ACTGGGA1346750.0238.692957
TGGGAAA1326700.0237.721629
CTGGGAA1372400.0233.359858
ACGGGGA21150.0218.625357
TGACGGG19800.0216.467455
CGTGACA16550.0215.942673
GGTGACT34550.0214.944783
GTGGTGA46150.0204.702871
GTGACGG21250.0203.470474
GACGGGG24850.0199.740526
CGTGACG15000.0197.874243
TGGTGAC46750.0193.924772
CGGGGAA24900.0182.770258
AGGGAAA20650.0179.357019
TGACAGG21500.0172.883855
Produced by FastQC (version 0.11.5)