FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914141
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTAGG12490.1366310011256469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA894900.0240.88567
TCGTGAC897800.0240.869422
GTGACTG897900.0240.726094
TGACTGG900700.0239.817735
TGGGAAA900200.0239.332929
GACTGGG907550.0237.661336
CGTGACT926750.0233.427283
CTGGGAA937500.0229.810688
TGCGAAA1250.0193.606319
ACGTGAC2450.0182.950822
CTGCGAA1450.0166.9028
TCGAGAC2050.0147.73552
GACTGGA3900.0133.41466
CGAGACT2750.0132.062093
GTCGTGA1743150.0124.02361
GCGTGGC2000.0121.1431052
ACGAGAC602.2737368E-9121.1431052
GACGTGA3750.0119.534411
ACTCGGA1250.0116.163797
GACTGGC1850.0111.192816
Produced by FastQC (version 0.11.5)