FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003045
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTGCGGGGTCGTGATTCAGAGTTGGCCAA22310.11138042330551737No Hit
GTCGTGACTGGGAAAACCCTGGGCGACAGTCGTGATCAATAAGGTGCCAA21530.1074863520290358No Hit
GTCGTGACTGGGAAAACCCTGGCTTCCTGTCGTGATACTTTAGGAGCCAA21290.10628817625165685No Hit
GTCGTGACTGGGAAAACCCTGGACACACGTCGTGATAATAGAGGAGCCAA20630.10299319286386477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG1898950.0241.402154
TCGTGAC1893250.0241.373952
TGACTGG1904300.0240.841085
GACTGGG1906400.0240.367316
CGTGACT1911200.0238.682533
ACTGGGA1908950.0238.240757
TGGGAAA1875350.0235.056039
CTGGGAA1961900.0233.78458
CGTGGCT55200.0213.505313
TCGTGGC44500.0212.677412
GTCGTGG62850.0152.787981
GCGTGGC22000.0127.2388152
GTGGCTG94850.0127.0063254
GGTGACT29000.0126.555073
GTCGTGA3717350.0122.993131
TGGCTGG104400.0115.713195
GGCTGGG126400.0113.664266
CGTTGCA551.9177787E-7110.113923
GCTGGGA70600.0105.768757
CGTGACA9150.0103.254373
Produced by FastQC (version 0.11.5)