FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908660
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGACTTG23810.12474720484528412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG1879750.0240.880265
GTGACTG1880250.0240.763574
TCGTGAC1885800.0240.519362
CGTGACT1884050.0240.431053
ACTGGGA1884650.0239.804957
GACTGGG1886600.0239.74286
CTGGGAA1921550.0235.149698
TGGGAAA1961800.0230.263479
GTCGTGA3668000.0123.6190261
TGACCGG6500.0107.9751365
ACCGGGA5400.0107.552987
TCGTGGC6750.087.945522
CGTGACC7800.082.261243
ACTAGGA3250.081.905747
GCGTGGC3450.080.76632
ACGTGAC5150.079.9821552
GAACCAG263550.078.63212240-242
AGAACCA266450.078.29097240-242
AACCAGG260050.076.41781240-242
CGAGTTA14250.075.28972240-242
Produced by FastQC (version 0.11.5)