FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093814
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG1967900.0241.635854
TGACTGG1968950.0241.419625
CGTGACT1936050.0241.144013
TCGTGAC1963400.0240.909712
GACTGGG1992650.0240.149266
ACTGGGA1982500.0239.874057
TGGGAAA1986150.0234.929899
CTGGGAA2019550.0234.401348
ACGGGGA24600.0221.424167
GACGGGG27200.0214.550496
CGTGACG17550.0214.031943
TGACGGG25400.0206.437485
CGTGACA27700.0191.596833
AGGGAAA30750.0182.645979
GTGACGG29600.0178.019244
CGTGGCT16750.0177.233283
GTGGTGA63750.0171.971471
GGCGTGA17250.0161.69881
GCGTGAC16450.0160.673542
TCGTGGC16850.0159.737472
Produced by FastQC (version 0.11.5)