FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597750
Sequences flagged as poor quality0
Sequence length248
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGTGGCCGTCGTGATGACAACGCCCAGTG13340.2231702216645755No Hit
GTCGTGACTGGGAAAACCCTGGCCAACCGTCGTGATCTGGTGTACCAGTG10010.16746131325805103No Hit
GTCGTGACTGGGAAAACCCTGGTATCGAGTCGTGATGTAGGAAAGCAGTG9190.15374320368046843No Hit
GTCGTGACTGGGAAAACCCTGGCCACTTGTCGTGATTCGTTCTTACAGTG8130.13601003764115432No Hit
GTCGTGACTGGGAAAACCCTGGCATTAGGTCGTGATGTAAGTCAGCAGTG7510.12563780844834796No Hit
GTCGTGACTGGGAAAACCCTGGCCATTAGTCGTGATGCGTGGATTCAGTG7480.12513592639063154No Hit
GTCGTGACTGGGAAAACCCTGGTCGCTTGTCGTGATTATTCTAAAAAGTA7230.12095357590966123No Hit
GTCGTGACTGGGAAAACCCTGGTCACCAGTCGTGATGGCGTGAGCCAGTG6740.11275616896695942No Hit
GTCGTGACTGGGAAAACCCTGGAAGCTTGTCGTGATATATACATTCAGTG6570.10991217063989961No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTCACTGGGGGGGAGACAGTG6540.10941028858218318No Hit
GTCGTGACTGGGAAAACCCTGGATTGAAGTCGTGATTGCCGTCATCAGTG6400.10706817231283981No Hit
GTCGTGACTGGGAAAACCCTGGGCACATGTCGTGATAAGAATGGGCAGTG6260.10472605604349644No Hit
GTCGTGACTGGGAAAACCCTGGCTAATTGTCGTGATTTCACCCCCCAGTG6160.10305311585110832No Hit
GTCGTGACTGGGAAAACCCTGGCTCACGGTCGTGATTTAATGAAACAGTG6100.10204935173567546No Hit
GTCGTGACTGGGAAAACCCTGGAAATTGGTCGTGATATAACCCCGCAGTG6050.10121288163948139No Hit
GTCGTGACTGGGAAAACCCTGGCGGATGGTCGTGATGGGTGAGCCCAGTG6050.10121288163948139No Hit
GTCGTGACTGGGAAAACCCTGGATCGTCGTCGTGATCCCATCTGGCAGTG6010.10054370556252615No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTT251.7316779E-9242.142643
TCGTGAC569800.0241.55762
ACTGGGA564200.0239.513067
TGGGAAA548500.0238.537389
CGTGACT576250.0238.444843
CTGGGAA573550.0235.903888
CGTGGCT5700.0227.274223
GTGACTG606000.0226.581194
TGACTGG603800.0226.369775
GACTGGG602650.0224.954566
TCGGACT305.122274E-9201.97152
CGTTACT305.149559E-9201.785543
TCGTTGC451.8189894E-12188.506712
TGGGAAC17350.0182.02379
GTGACTT3350.0162.60594
CGGACTT301.2482851E-6161.428423
TCGTGGC6400.0153.372122
CGTGACA2450.0153.192283
TCGTTAC750.0145.419462
GTCGTGA1001950.0137.474731
Produced by FastQC (version 0.11.5)