FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508973
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTAGG8620.16936065370854642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG498100.0240.930954
TCGTGAC498400.0240.927982
TGACTGG498900.0240.47375
ACTGGGA497750.0240.372947
TGGGAAA501200.0238.911459
GACTGGG501350.0238.743426
CTGGGAA515750.0231.983758
CGTGACT520900.0230.499713
CGAGACT1600.0174.041293
TCGAGAC1700.0163.85192
ACGTGAC1150.0157.966862
TCGACTG251.2185841E-4145.32952
GACTGGA2550.0132.863666
GTCGGAC1050.0126.949881
GACTGGC1450.0125.173276
GTCGTGA968300.0124.045281
CGTAACT405.2232754E-6121.0722053
ACCGGGA1200.0121.000847
GACGTGA1500.0113.100791
GTGACCG1150.0105.249144
Produced by FastQC (version 0.11.5)