FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2698655
Sequences flagged as poor quality0
Sequence length248
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGTGAAAGTCGTGATCTAAATGCCTTAGG29470.10920254719480631No Hit
GTCGTGACTGGGAAAACCCTGGAGGAGCGTCGTGATCCCTCGGAGTTAGG27370.10142089300040205No Hit
GTCGTGACTGGGAAAACCCTGGCTTCATGTCGTGATCGGTACACTTTAGG27290.10112444903109141No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC2472500.0241.743292
ACTGGGA2490750.0241.277627
GTGACTG2504950.0240.294754
TGACTGG2500600.0240.156945
TGGGAAA2450050.0240.109289
GACTGGG2524100.0239.447546
CGTGACT2519000.0234.128573
CTGGGAA2577800.0232.347528
ACGGGGA42400.0226.419577
CGTGACA32250.0225.845063
GTGACGG41400.0218.318974
GACGGGG48400.0217.409076
TGACGGG41300.0217.0235
GTGGTGA78300.0215.644531
TGACAGG35400.0211.108645
TGGTGAC76900.0207.520722
CGTGACG28750.0207.392533
GGTGACG16000.0194.661013
TCGGGAC24150.0189.804532
CGGGGAA49300.0189.80128
Produced by FastQC (version 0.11.5)