FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806121
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTCGTCAGTCGTGATGTCAGGTGATAGCT32000.39696273884441663No Hit
GTCGTGACTGGGAAAACCCTGGCTCCATGTCGTGATAATAGCCAGTAGCT31200.38703867037330625No Hit
GTCGTGACTGGGAAAACCCTGGCGCTCTGTCGTGATTAAAACCCTTAGCT30010.37227661852252947No Hit
GTCGTGACTGGGAAAACCCTGGCCTCCCGTCGTGATCTATATAATTAGCT28090.34845885419186445No Hit
GTCGTGACTGGGAAAACCCTGGAAGCTCGTCGTGATACAGTTGGATAGCT26400.32749425954664374No Hit
GTCGTGACTGGGAAAACCCTGGGGTCTGGTCGTGATAGGTACAAGTAGCT26090.32364868301408845No Hit
GTCGTGACTGGGAAAACCCTGGATGTTGGTCGTGATCGCGAAACCTAGCT23570.29238786733009065No Hit
GTCGTGACTGGGAAAACCCTGGAGTACCAGTCGTGATCGGTAGTTTAGCT21240.26348401790798154No Hit
GTCGTGACTGGGAAAACCCTGGCCGTCGGTCGTGATATCATCCAATAGCT21050.26112705164609284No Hit
GTCGTGACTGGGAAAACCCTGGACTCTCGTCGTGATTGATAGATCTAGCT20380.25281564430153786No Hit
GTCGTGACTGGGAAAACCCTGGTGTCATGTCGTGATTCTTGATGCTAGCT19940.2473574066424271No Hit
GTCGTGACTGGGAAAACCCTGGCACTTAGTCGTGATATGGAGTTGTAGCT19290.23929410100964993No Hit
GTCGTGACTGGGAAAACCCTGGACCAATGTCGTGATTACCATGACTAGCT18970.23532447362120573No Hit
GTCGTGACTGGGAAAACCCTGGGTGATGGTCGTGATGGACTCACCTAGCT17770.2204383709145401No Hit
GTCGTGACTGGGAAAACCCTGGGAATCTGTCGTGATGCTAAGACTTAGCT16280.20195479338709696No Hit
GTCGTGACTGGGAAAACCCTGGTGACGGGTCGTGATAAATACGATTAGCT16230.2013345391076526No Hit
GTCGTGACTGGGAAAACCCTGGAAGAACGTCGTGATGGAACCTTGTAGCT15760.1955041488808752No Hit
GTCGTGACTGGGAAAACCCTGGGCGCATGGTCGTGATAGTTCACATAGCT14850.18421552099498711No Hit
GTCGTGACTGGGAAAACCCTGGGTACGCGTCGTGATACTGTGGAATAGCT14840.18409147013909824No Hit
GTCGTGACTGGGAAAACCCTGGAGTTGTGTCGTGATACTGAGAAGTAGCT14250.1767724696416543No Hit
GTCGTGACTGGGAAAACCCTGGTTTGTGGTCGTGATGGTGGGGAATAGCT13990.1735471473885434No Hit
GTCGTGACTGGGAAAACCCTGGCTCCGCGTCGTGATATCCCGCCCTAGCT13690.169825621711877No Hit
GTCGTGACTGGGAAAACCCTGGCCGCTGGTCGTGATGCAGAAACTTAGCT13380.16598004517932172No Hit
GTCGTGACTGGGAAAACCCTGGACGATGGTCGTGATCCGTATTCATAGCT12930.1603977566643221No Hit
GTCGTGACTGGGAAAACCCTGGCGTCACGTCGTGATTCTGAGATTTAGCT12740.15804079040243338No Hit
GTCGTGACTGGGAAAACCCTGGCTAGGCGTCGTGATTATAAGGGGTAGCT12390.1536990104463226No Hit
GTCGTGACTGGGAAAACCCTGGCTGCCTGTCGTGATAGCCAGTAATAGCT12310.15270660359921154No Hit
GTCGTGACTGGGAAAACCCTGGCGTTGTGTCGTGATCACTGGATGTAGCT12280.15233445103154492No Hit
GTCGTGACTGGGAAAACCCTGGTTCATTGTCGTGATCGGGAGTTGTAGCT11500.14265848427221223No Hit
GTCGTGACTGGGAAAACCCTGGGCTGGGGTCGTGATCTAAAAACATAGCT11350.14079772143387903No Hit
GTCGTGACTGGGAAAACCCTGGGCGATGGTCGTGATGTCATGTAATAGCT11250.13955721287499023No Hit
GTCGTGACTGGGAAAACCCTGGGATTCCGTCGTGATGCTAGATACTAGCT11110.1378205008925459No Hit
GTCGTGACTGGGAAAACCCTGGTACAGCGTCGTGATATACATGGATAGCT11100.13769645003665704No Hit
GTCGTGACTGGGAAAACCCTGGCTCGTCGTCGTGATTGCATTGCTTAGCT10550.13087365296276862No Hit
GTCGTGACTGGGAAAACCCTGGACCTGGGTCGTGATATAATAGCGTAGCT10500.13025339868332422No Hit
GTCGTGACTGGGAAAACCCTGGCAATCGGTCGTGATCCATTGTGGTAGCT10460.1297571952597687No Hit
GTCGTGACTGGGAAAACCCTGGTCTGATGTCGTGATCCTTTGATATAGCT10240.12702807643021333No Hit
GTCGTGACTGGGAAAACCCTGGTCATCTGTCGTGATTGATTTCCATAGCT10210.12665592386254668No Hit
GTCGTGACTGGGAAAACCCTGGGCCAATGTCGTGATAGAGAGGGATAGCT10150.1259116187272134No Hit
GTCGTGACTGGGAAAACCCTGGGTCTGAGTCGTGATAAGGCCTGATAGCT10030.12442300845654684No Hit
GTCGTGACTGGGAAAACCCTGGTGCTGTGTCGTGATATTTATTACTAGCT9840.12206604219465811No Hit
GTCGTGACTGGGAAAACCCTGGCATAAAGTCGTGATCGCCTCGACTAGCT9820.12181794048288035No Hit
GTCGTGACTGGGAAAACCCTGGGCCTATGTCGTGATGCAAAATACTAGCT9450.1172280588149918No Hit
GTCGTGACTGGGAAAACCCTGGCTGAGCGTCGTGATGCTTGACTATAGCT9400.11660780453554741No Hit
GTCGTGACTGGGAAAACCCTGGGACAATGTCGTGATGGGCCGAGATAGCT9380.11635970282376965No Hit
GTCGTGACTGGGAAAACCCTGGAATGTCGTCGTGATCACCATGATTAGCT9360.11611160111199188No Hit
GTCGTGACTGGGAAAACCCTGGGTTCAGGTCGTGATTGACTATACTAGCT9330.11573944854432525No Hit
GTCGTGACTGGGAAAACCCTGGCGCGTCGTCGTGATCCAGATCGGTAGCT9190.11400273656188091No Hit
GTCGTGACTGGGAAAACCCTGGAGGCACGTCGTGATTGTGGAGTTTAGCT9160.11363058399421427No Hit
GTCGTGACTGGGAAAACCCTGGTCTCCTGTCGTGATGGCTTCGCTTAGCT9160.11363058399421427No Hit
GTCGTGACTGGGAAAACCCTGGACCTGGGTCGTGATAAACGGGGGTAGCT9140.11338248228243651No Hit
GTCGTGACTGGGAAAACCCTGGCCTCAGGTCGTGATGCTGTCGATTAGCT9140.11338248228243651No Hit
GTCGTGACTGGGAAAACCCTGGCGAAGCGTCGTGATGGTATATACTAGCT9040.11214197372354771No Hit
GTCGTGACTGGGAAAACCCTGGGAACTCGTCGTGATGCCAGAGAATAGCT9020.11189387201176995No Hit
GTCGTGACTGGGAAAACCCTGGCCAATTGTCGTGATCATGTGTACTAGCT8990.11152171944410331No Hit
GTCGTGACTGGGAAAACCCTGGTCTGCGGTCGTGATCAGAACGAGTAGCT8930.11077741430877003No Hit
GTCGTGACTGGGAAAACCCTGGAACTCCGTCGTGATGACACAATGTAGCT8930.11077741430877003No Hit
GTCGTGACTGGGAAAACCCTGGGAACATGTCGTGATTTGTGTGGGTAGCT8840.1096609566057701No Hit
GTCGTGACTGGGAAAACCCTGGCCCGCCGTCGTGATCGTGAGACGTAGCT8790.1090407023263257No Hit
GTCGTGACTGGGAAAACCCTGGGTCGAAGTCGTGATAGATACCTCTAGCT8760.10866854975865907No Hit
GTCGTGACTGGGAAAACCCTGGCGTGGGGTCGTGATGGAGACATCTAGCT8750.10854449890277018No Hit
GTCGTGACTGGGAAAACCCTGGCACAAAGTCGTGATGTATGGTACTAGCT8530.10581538007321481No Hit
GTCGTGACTGGGAAAACCCTGGATGCCAGTCGTGATGAGTACTCTTAGCT8410.10432676980254825No Hit
GTCGTGACTGGGAAAACCCTGGCTGCGTGTCGTGATTGACCACCTTAGCT8320.10321031209954833No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGTGTCGTGATACCCACGGGTAGCT8290.10283815953188169No Hit
GTCGTGACTGGGAAAACCCTGGATATTGGTCGTGATGGCAAGATTTAGCT8250.10234195610832617No Hit
GTCGTGACTGGGAAAACCCTGGTTGTATGTCGTGATCCTAGATACTAGCT8230.1020938543965484No Hit
GTCGTGACTGGGAAAACCCTGGTACATGGTCGTGATGGCAAGTACTAGCT8210.10184575268477064No Hit
GTCGTGACTGGGAAAACCCTGGTCCATCGTCGTGATTCAGTTATCTAGCT8140.10097739669354848No Hit
GTCGTGACTGGGAAAACCCTGGTAGTACGTCGTGATTTCAAGGGCTAGCT8110.10060524412588184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC767500.0241.500582
CGTGACT766900.0241.217863
TGACTGG766200.0241.166065
GTGACTG766350.0241.145484
TCGTGGC14050.0240.675452
GACTGGG767050.0240.12726
ACTGGGA762700.0239.815387
CTGGGAA779150.0236.214268
TGGGAAA765450.0235.872579
CGTGGCT17250.0230.413383
CGTGACA1750.0228.506653
CGTTGCA351.2876626E-8173.111083
ACTGGGG8850.0143.609657
GTGGCTG30100.0135.594994
GGTGACT3850.0132.193923
GTGACTT5450.0131.109954
GGCGTGG7350.0128.716751
GTCTGAC2400.0126.3445741
GCGTGGC7200.0126.250372
GTCGTGA1516600.0122.2824861
Produced by FastQC (version 0.11.5)