FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139776
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGATTGGAGTCGTGATTAGAATTACACTTG62490.5482656241226346No Hit
GTCGTGACTGGGAAAACCCTGGCCCAATGTCGTGATAATAACAAAACTTG54420.47746223819417144No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGACTTG16370.14362471222415632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1121050.0241.011282
CGTGACT1119850.0240.9813
TGACTGG1119100.0240.97185
GTGACTG1119250.0240.960654
ACTGGGA1123300.0239.651217
GACTGGG1124600.0239.57866
CTGGGAA1153050.0233.310538
TGGGAAA1162150.0231.504479
TGACCGG2900.0179.421075
ACCGGGA3150.0145.961387
TCGTGGC3050.0135.038832
CGTGACC3650.0126.033013
GTCGTGA2184300.0123.6598741
CGTGGCT4050.0122.5525743
ACGTGAC2700.0116.65122
TCGTTAC1300.0102.50122
GACGTGA3050.099.3412551
GACCGGG5550.095.923416
GACTGCG1800.094.106676
CGGGGAA3800.092.337759
Produced by FastQC (version 0.11.5)