FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149670
Sequences flagged as poor quality0
Sequence length248
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCTTTACGTCGTGATACGCACTAGCAGAT145279.706019910469701No Hit
GTCGTGACTGGGAAAACCCTGGGCGGGGGTCGTGATTAGAATACACAGAT87585.851540054787199No Hit
GTCGTGACTGGGAAAACCCTGGGACAACGTCGTGATCAAAATGGCCAGAT72144.819937195162692No Hit
GTCGTGACTGGGAAAACCCTGGCAGTTTGTCGTGATATTAACTTGCAGAT60104.015500768357052No Hit
GTCGTGACTGGGAAAACCCTGGTGGCCAGTCGTGATTTTACGGTGCAGAT50713.3881205318367074No Hit
GTCGTGACTGGGAAAACCCTGGATGTGGGTCGTGATCTAAATTCCCAGAT39822.660519810249215No Hit
GTCGTGACTGGGAAAACCCTGGTTTGAGTCGTGATGCGAAGATGGCAGAT37132.4807910736954635No Hit
GTCGTGACTGGGAAAACCCTGGAGAATAGTCGTGATTGACTAGGTCAGAT36752.4554018841451195No Hit
GTCGTGACTGGGAAAACCCTGGCTGACCGTCGTGATTAGTCATCTCAGAT29801.991046970000668No Hit
GTCGTGACTGGGAAAACCCTGGTTTGAGTCCTGATGCGAAGATGGCAGAT27821.858755929712033No Hit
GTCGTGACTGGGAAAACCCTGGTACAAGGTCGTGATGTCTGGGTGCAGAT17721.1839379969265718No Hit
GTCGTGACTGGGAAAACCCTGGTCGACCGTCGTGATGTTCGTTTCCAGAT16781.121133159617826No Hit
GTCGTGACTGGGAAAACCCTGGAGCGGGGTCGTGATACGGAGCCCCAGAT14280.9540990178392463No Hit
GTCGTGACTGGGAAAACCCTGGTTTGAGTCCTGATTCGAAGATGGCAGAT10450.6982027126344625No Hit
GTCGTGACTGGGAAAACCCGGGTTTGAGTCGTGATGCGAAGATGGCAGAT9420.6293846462216878No Hit
GTCGTGACTGGGAAAACCCGGGCTTTACGTCGTGATACGCACTAGCAGAT9070.6059998663726865No Hit
GTCGTGACTGGGAAAACCCGGGGCGGGGGTCGTGATTAGAATACACAGAT8040.5371817999599118No Hit
GTCGTGACTGGGAAAACCCGGGCAGTTTGTCGTGATATTAACTTGCAGAT5590.37348834101690387No Hit
GTCGTGACTGGGAAAACCCGGGATGTGGGTCGTGATCTAAATTCCCAGAT4500.30066145520144316No Hit
GTCGTGACTGGGAAAACCCTGGCCGGATGTCGTGATGCTGAGGTCCAGAT4370.291975679828957No Hit
GTGGTGACTGGGAAAACCCTGGGCGGGGGTCGTGATTAGAATACACAGAT3910.2612413977416984No Hit
GTGGTGACTGGGAAAACCCTGGCTTTACGTCGTGATACGCACTAGCAGAT3880.25923698804035544No Hit
GTCGTGACTGGGAAAACCCGGGTGGCCAGTCGTGATTTTACGGTGCAGAT3690.24654239326518343No Hit
GTCGTGACTGGGAAAACCCGGGTTTGAGTCCTGATGCGAAGATGGCAGAT3540.23652034475846864No Hit
GTCGTGACTGGGAAAACCCTGGTCGACCGTCGTGATGTTCGTTTACAGAT3270.21848065744638204No Hit
GTCGTGACTGGGAAAACCCTGGCCGGATGTCGTGATGCTGAGGACCAGAT2910.19442774103026658No Hit
GTCGTGACTGGGAAAACCCGGGGACAACGTCGTGATCAAAATGGCCAGAT2780.18574196565778045No Hit
GTCGTGACTGGGAACACCCTGGCTTTACGTCGTGATACGCACTAGCAGAT2670.17839246341952295No Hit
GTGGTGACTGGGAAAACCCTGGGACAACGTCGTGATCAAAATGGCCAGAT2550.17037482461415115No Hit
GTGGTGACTGGGAAAACCCGGGTTTGAGTCGTGATGCGAAGATGGCAGAT2450.16369345894300794No Hit
GTCGTGACTGGGAAAACCCTGGGTGAGCGTCGTGATGAGGAACTGCAGAT2440.16302532237589365No Hit
GTGGTGACTGGGAAAACCCTGGCAGTTTGTCGTGATATTAACTTGCAGAT2190.14632190819803567No Hit
GTGGTGACTGGGAAAACCCGGGCTTTACGTCGTGATACGCACTAGCAGAT2110.14097681566112114No Hit
GTGGTGACTGGGAAAACCCTGGTTTGAGTCGTGATGCGAAGATGGCAGAT2080.13897240595977817No Hit
GTCGTGACTGGGAAACCCCTGGCTTTACGTCGTGATACGCACTAGCAGAT2030.1356317231242066No Hit
GTGGTGACTGGGAAAACCCGGGGCGGGGGTCGTGATTAGAATACACAGAT2020.13496358655709229No Hit
GTCGTGACAGGGAAAACCCTGGCTTTACGTCGTGATACGCACTAGCAGAT1810.12093271864769158No Hit
GTCGTGACTGGGAAAACCCTGGGCGGGGGTCGTGATTAGAAAACACAGAT1740.11625576267789135No Hit
GTCGTGACTGGGAAAACCCTGGTTTGAGTCGTGATTCGAAGATGGCAGAT1740.11625576267789135No Hit
GTCGTGACGGGGAAAACCCTGGGCGGGGGTCGTGATTAGAATACACAGAT1720.11491948954366273No Hit
GTCGTGACTGGGAAAACCCTGGCTGTAAGTCGTGATACTGTGTTCCAGAT1550.10356116790271931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG102100.0242.153474
TTCTAGA100.0015274392242.153464
GAGTGTG100.0015274392242.153461
TCGCATG151.5891388E-5242.153463
GTTCTGT100.0015274392242.153461
TTAATTA100.0015274392242.153464
CGAGTGA301.8189894E-11242.153464
ACTGAGA100.0015274392242.153464
ACTGAAG100.0015274392242.153463
CGTGAGT800.0242.153463
CGTGAGA100.0015274392242.153464
GGCCGTA151.5891388E-5242.153461
GACCGAA151.5891388E-5242.153461
GCAAATG151.5891388E-5242.153463
GCACGAG201.6506965E-7242.153463
GTCTAGA100.0015274392242.153464
CAATTGA100.0015274392242.153464
TGAGTGA100.0015274392242.153464
CTGACTG100.0015274392242.153464
GACCAAT100.0015274392242.153461
Produced by FastQC (version 0.11.5)