FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325287
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1296850.0241.606263
TGACTGG1299500.0241.28345
GTGACTG1299300.0241.198154
TCGTGAC1301750.0241.140272
ACTGGGA1303400.0240.256457
GACTGGG1306500.0240.019876
CTGGGAA1339050.0233.932538
TGGGAAA1384500.0226.165869
CGTGGCT6450.0155.848683
ACGTGAC1950.0136.721022
CGTGACA4500.0129.185413
TCGAGAC3950.0125.78652
GTCGTGA2535200.0123.7880551
CGAGACT3750.0119.49653
TCGTGGC8000.0107.5512852
AGGGAAA7300.0106.103659
TGCGAAA1650.0102.687439
TGACAGG6250.096.852485
GACTGCG1850.091.5936
GTGACTA1500.088.801554
Produced by FastQC (version 0.11.5)