FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297614
Sequences flagged as poor quality0
Sequence length248
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA2191350.0241.263087
TCGTGAC2214600.0240.801222
TGGGAAA2150300.0240.505979
GTGACTG2216250.0240.383944
TGACTGG2216450.0240.156345
GACTGGG2220400.0239.22346
CGTGACT2234800.0238.314363
CTGGGAA2268800.0233.93418
CGTGGCT33650.0218.082693
ACGTGAC2050.0171.375232
GCGTGGC10750.0163.40432
TCGTGGC39450.0153.8492
GTGGTGA13550.0152.049131
GACTGGA12200.0143.835986
GTCGTGG48700.0127.413451
GTCGTGA4241300.0125.8154141
TCGGGAC6200.0121.144572
TGACAGG9000.0121.03385
GTGACTT16550.0118.4922264
TGCGAAA3450.0115.75139
Produced by FastQC (version 0.11.5)