FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370480
Sequences flagged as poor quality0
Sequence length248
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGATCAC15750.11492323857334656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1350900.0240.834082
ACTGGGA1349900.0240.516457
TGGGAAA1355150.0239.557719
GTGACTG1369150.0237.468934
TGACTGG1370400.0237.112085
GACTGGG1378700.0235.518596
CGTGACT1382250.0235.243123
CTGGGAA1401100.0231.709878
TCGAGAC4000.0172.648652
ACGTGAC2750.0149.794042
CGAGACT4800.0136.221943
CTGCGAA1600.0136.132468
GCGTGGC2150.0129.609762
GTGACCG3000.0125.081274
TGCGAAA1750.0124.463989
GTCGTGA2628500.0123.728351
CGGACTG1000.0121.086173
TCGTTAC1350.0116.669662
ACTCGGA1450.0100.139777
GACTGGA7050.094.39896
Produced by FastQC (version 0.11.5)