FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965807
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTAGG12640.13087500918920653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC946400.0241.339452
ACTGGGA943450.0241.296057
TGGGAAA949800.0239.721029
GTGACTG953250.0239.448234
TGACTGG954250.0239.135075
GACTGGG957250.0238.082876
CGTGACT979650.0233.141193
CTGGGAA1001100.0227.38858
ACGTGAC2200.0198.339772
TCGAGAC2600.0186.473272
CGAGACT3300.0150.443073
GACTGGA4700.0149.312096
ACTGGAA5100.0137.601357
TGCGAAA1450.0133.510939
TTGTGAC1350.0125.696812
GTCGTGA1837450.0124.3037951
CTGCGAA1600.0120.994288
GACGTGA3400.0117.679321
CGGGGAA3600.0117.633329
AATGGGA1950.0111.687037
Produced by FastQC (version 0.11.5)