FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094045
Sequences flagged as poor quality0
Sequence length248
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA1043400.0241.055957
TCGTGAC1051000.0240.925482
TGGGAAA1020500.0240.038259
TGACTGG1056000.0239.323755
GTGACTG1058400.0239.019184
GACTGGG1059000.0238.349616
CGTGACT1063300.0237.538823
CGTGGCT12050.0234.168383
CTGGGAA1078850.0233.79758
GTGACTT4700.0208.654314
GCGTGGC4450.0196.01572
TCGGACT1000.0181.722892
TCGTGGC13750.0162.118852
TGCGAAA1400.0155.59879
CGTGGCA1000.0145.32513
CGTAACT251.2191116E-4145.32513
CTGCGAA1500.0145.225458
GTGGTGA7300.0142.81451
TCGTTGC850.0142.527762
GTCGTAC1050.0138.54431
Produced by FastQC (version 0.11.5)