FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3886335
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTTCTTCGTCGTGATGCAATGAGAGCCAA56570.14556130647512372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT3825450.0241.323673
GTGACTG3826500.0241.201494
TGACTGG3829700.0240.994835
TCGTGAC3838100.0240.80952
ACTGGGA3840800.0239.84517
GACTGGG3847400.0239.55316
CTGGGAA3913150.0235.373528
TGGGAAA3990900.0230.803289
ACGTGAC6600.0172.53972
CGAGACT8300.0147.32243
ACCGGGA8150.0146.979647
GTCGTGA7488700.0123.384861
CGGGGAA8150.0114.314569
TCGAGAC10200.0114.018742
CGTGGCT15550.0104.3277743
GACGTGA9700.0103.686791
TGCGAAA6150.0100.337549
GTGACTA4600.097.358614
ACGGGAA5600.092.9094857
TCGTGGC17750.089.408352
Produced by FastQC (version 0.11.5)