FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930210
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1835050.0241.808433
TGACTGG1839050.0241.298285
GTGACTG1840450.0241.182824
TCGTGAC1844050.0241.061772
GACTGGG1846750.0240.26666
ACTGGGA1851150.0238.107647
CTGGGAA1905500.0232.942938
TGGGAAA1865200.0232.453869
TCGTGGC33050.0221.14482
CGTGGCT40550.0217.786483
ACTGGGG18050.0163.619587
GTGACTT10800.0149.125954
GCGTGGC13750.0146.330982
TGACTTG11500.0140.001535
GTGGCTG63350.0139.923224
TTGGAAA12650.0135.8629
GGCGTGG15500.0132.996381
CGTGACA7600.0132.299673
ACTTGGA13050.0131.704487
GCGGGGC13800.0126.4778062
Produced by FastQC (version 0.11.5)