FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724825
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG1626500.0241.680984
TGACTGG1626800.0241.632455
TCGTGAC1627200.0241.333182
CGTGACT1623650.0241.217863
GACTGGG1637750.0239.922556
ACTGGGA1626750.0239.617697
CTGGGAA1684700.0233.352048
CGTGGCT48000.0228.65633
TGGGAAA1661750.0227.761559
TCGTGGC37850.0222.21142
GCGTGGC14150.0210.693622
GGCGTGG16000.0187.203781
GTCGTGG46650.0183.263141
GTGGCTG62700.0177.67034
TGGCTGG67300.0166.027655
ACTGGGG19750.0162.41357
GCGGGGC11950.0159.22252
TTGGAAA10250.0146.425269
GCTGGGA47850.0139.6377
CTGGGGG51200.0131.911428
Produced by FastQC (version 0.11.5)