FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13818
Sequences flagged as poor quality0
Sequence length248
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGGGTACGTCGTGATATTCACTCCCAGTG1090.7888261687653785No Hit
GTCGTGACTGGGAAAACCCTGGCGGACCGTCGTGATGGGAGCCCTCAGTG1070.774352294109133No Hit
GTCGTGACTGGGAAAACCCTGGTCCCATGTCGTGATCAAGTACATACAGT1040.7526414821247649No Hit
GTCGTGACTGGGAAAACCCTGGTATTGCGTCGTGATAGTACCTCTACTGC1000.7236937328122739No Hit
GTCGTGACTGGGAAAACCCTGGAGACATGTCGTGATGATAATGAGCAGTG960.6947459834997829No Hit
GTCGTGACTGGGAAAACCCTGGGCTAACGTCGTGATTCAAAGTTTCAGTG920.665798234187292No Hit
GTCGTGACTGGGAAAACCCTGGTTCCGGGTCGTGATAAAAATATCACAGT920.665798234187292No Hit
GTCGTGACTGGGAAAACCCTGGCCATTGGTCGTGATTGGAGGGAGCAGTG810.5861919235779418No Hit
GTCGTGACTGGGAAAACCCTGGCTACTAGTCGTGATAAAAATGCTCCAGG810.5861919235779418No Hit
GTCGTGACTGGGAAAACCCTGGAGCCCGGTCGTGATTGTTATCGCACAGT790.5717180489216963No Hit
GTCGTGACTGGGAAAACCCTGGAACGTAGTCGTGATAAAGGCTGCACAGT780.5644811115935736No Hit
GTCGTGACTGGGAAAACCCTGGATTGCTGTCGTGATCTGATAATCCAGTG690.499348675640469No Hit
GTCGTGACTGGGAAAACCCTGGGTTCAAGTCGTGATGCGGAGATGACAGT670.4848748009842235No Hit
GTCGTGACTGGGAAAACCCTGGGAGCAGGTCGTGATGTGGGTGATACAGT670.4848748009842235No Hit
GTCGTGACTGGGAAAACCCTGGCCGTCCGTCGTGATCAATAGTGACAGTG620.4486901143436098No Hit
GTCGTGACTGGGAAAACCCTGGCAAATCGTCGTGATGCCCCCGTCCAGTG570.4125054277029961No Hit
GTCGTGACTGGGAAAACCCTGGACAATAGCGTGATCGTTGGATGCGCAGG570.4125054277029961No Hit
GTCGTGACTGGGAAAACCCTGGAGAAGGGTCGTGACGAAACGACTCAGTG550.3980315530467506No Hit
GTCGTGACTGGGAAAACCCTGGATTATGGTCGTGATTAACGATTGACAGT550.3980315530467506No Hit
GTCGTGACTGGGAAAACCCTGGTTTTTAGTCGTGATTATTATGGTACAGT550.3980315530467506No Hit
GTCGTGACTGGGAAAACCCTGGCTAGTTGCGTAATCCGATCGACAGCGGT540.39079461571862784No Hit
GTCGTGACTGGGAAAACCCTGGCCAGCTGTCGTGATTTGGTGTTAACAGT500.36184686640613695No Hit
GTCGTGACTGGGAAAACCCTGGTTTCACGTCGTGACATGATGAAAACAGT480.34737299174989145No Hit
GTCGTGACTGGGAAAACCCTGGCTAACAGTCGTGATTAAACTGCCCAGTG480.34737299174989145No Hit
GTCGTGACTGGGAAAACCCTGGGGATCCGTCGTGATGCAGGAGGGCAGTG470.3401360544217687No Hit
GTCGTGACTGGGAAAACCCTGGACCACAGTCGTGATGGATTTGCTACAGT460.332899117093646No Hit
GTCGTGACTGGGAAAACCCTGGGCGCTAGTCGTGATGCAATACCCCAGTG450.3256621797655232No Hit
GTCGTGACTGGGAAAACCCTGGCATTCGGTCGTGATGGTAATAGTACAGT450.3256621797655232No Hit
GTCGTGACTGGGAAAACCCTGGTGTCACGTCGTGATTCAAGCGGTACAGT450.3256621797655232No Hit
GTCGTGACTGGGAAAACCCTGGCTGAAGTCGTGATTGTAAGTTGGACAGT440.3184252424374005No Hit
GTCGTGACTGGGAAAACCCTGGAGCCCGGTCGTGATTGTTATAGCACAGT440.3184252424374005No Hit
GTCGTGACTGGGAAAACCCTGGCAAGCCGTCGTGATATTGAATAGACAGT440.3184252424374005No Hit
GTCGTGACTGGGAAAACCCTGGACTCTAGTCGTGATCCGTACAATCAGTG430.31118830510927775No Hit
GTCGTGACTGGGAAAACCCTGGCATACCGTCGTGATATGATTTGACAGTG430.31118830510927775No Hit
GTCGTGACTGGGAAAACCCTGGAAGGTGGTCGTGATGGGGTCGTCACAGT430.31118830510927775No Hit
GTCGTGACTGGGAAAACCCTGGCTTCCTGTCGTGACTTGGCGGAGACAGT430.31118830510927775No Hit
GTCGTGACTGGGAAAACCCTGGCCGCTCGTCGTGATATAATACCCACAGT420.303951367781155No Hit
GTCGTGACTGGGAAAACCCTGGTTCCGTGTCGTGATGGCCACGGGCAGTG420.303951367781155No Hit
GTCGTGACTGGGAAAACCCTGGCTACGGGTCGTGATGGGAGCGTGACAGT410.2967144304530323No Hit
GTCGTGACTGGGAAAACCCTGGCCCGCAGTCGTGATACATAGGATACAGT400.28947749312490956No Hit
GTCGTGACTGGGAAAACCCTGGCCGCCCGTCGTGATAATGTGTGCTCATT390.2822405557967868No Hit
GTCGTGACTGGGAAAACCCTGGCATTCAGTCGTGATCCATAGTACCAGTG380.27500361846866406No Hit
GTCGTGACTGGGAAAACCCTGGCGATATGTCGTGATCTGTTATACCAGTG370.2677666811405413No Hit
GTCGTGACTGGGAAAACCCTGGGCTTCCGTCGTGATGAAAACGGCACAGT370.2677666811405413No Hit
GTCGTGACTGGGAAAACCCTGGTGTCCTGTCGTGATCGCGGTTACCAGTG370.2677666811405413No Hit
GTCGTGACTGGGAAAACCCTGGTTAATAGTCGTGATAGCACAGTGCAGTG360.26052974381241856No Hit
GTCGTGACTGGGAAAACCCTGGACTCCGGTCGTGATGTAAAGTTGACAGT360.26052974381241856No Hit
GTCGTGACTGGGAAAACCCTGGGCTCCTGTCGTGATTAAAAACCTCCAGG360.26052974381241856No Hit
GTCGTGACTGGGAAAACCCTGGCACTTAGTCGTGATCGAGTCACCACAGT350.25329280648429586No Hit
GTCGTGACTGGGAAAACCCTGGCCGTAAGTCGTGATTCTAAGTGGACAGT350.25329280648429586No Hit
GTCGTGACTGGGAAAACCCTGGCAGATGGTCGTGATTGGTGTGGTCAGTG350.25329280648429586No Hit
GTCGTGACTGGGAAAACCCTGGTCCACTGTCGTGATAATAACGCCACAAA350.25329280648429586No Hit
GTCGTGACTGGGAAAACCCTGGACTCAAGTCGTGATGCTGGATCACAGTG350.25329280648429586No Hit
GTCGTGACTGGGAAAACCCTGGTCGATCGTCGTGATTGCGTGGAGCAGTG340.24605586915617308No Hit
GTCGTGACTGGGAAAACCCTGGGACCGCGTCGTGATTCTAACGTCACAGT340.24605586915617308No Hit
GTCGTGACTGGGAAAACCCTGGAGCGTAGTCGTGATAAAATGGTGACAGT340.24605586915617308No Hit
GTCGTGACTGGGAAAACCCTGGCCCATAGTCGTGATATCGCGAAGATGGT340.24605586915617308No Hit
GTCGTGACTGGGAAAACCCTGGGCCCTGGTCGTGATAAATATGCCCAGTG330.23881893182805036No Hit
GTCGTGACTGGGAAAACCCTGGACCTTTGTCGTGATTTGAAATGGCAGTG330.23881893182805036No Hit
GTCGTGACTGGGAAAACCCTGGGTGTAAGTCGTGATCAAGATGGAAAGTG330.23881893182805036No Hit
GTCGTGACTGGGAAAACCCTGGACACATGTCGTGATTAAAATTACACAGT320.2315819944999276No Hit
GTCGTGACTGGGAAAACCCTGGCGTTTCGTCGTGATCATTATGGAACAGT320.2315819944999276No Hit
GTCGTGACTGGGAAAACCCTGGTACATCGTCGTGATCAGGCCACTCAGTG320.2315819944999276No Hit
GTCGTGACTGGGAAAACCCTGGCATCATGACAACTCTATTATCAACAGTG320.2315819944999276No Hit
GTCGTGACTGGGAAAACCCTGGCCGAGTGTCGTGATTGAATGGCCCAGTG310.2243450571718049No Hit
GTCGTGACTGGGAAAACCCTGGCCGCTCGTCGTGATAACAGCCCCCAGTG310.2243450571718049No Hit
GTCGTGACTGGGAAAACCCTGGGTACACGTCGTGATGTGTTCGGCCAGTG300.21710811984368217No Hit
GTCGTGACTGGGAAAACCCTGGCTAACCGTCGTGATGATAACGCCAAAGT300.21710811984368217No Hit
GTCGTGACTGGGAAAACCCTGGTAAACTGTCGTGATTGTTAGATGACAGT300.21710811984368217No Hit
GTCGTGACTGGGAAAACCCTGGTAAATAGTCGTGATCCAAATGGGCAGTG300.21710811984368217No Hit
GTCGTGACTGGGAAAACCCTGGAATCTTGTCGTGATAACAATCTTACAGT300.21710811984368217No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGGGTCGTGATGTGAACATGACAGT290.20987118251555942No Hit
GTCGTGACTGGGAAAACCCTGGAGATCAGTCGTTGATTTGAATTTACAGT280.2026342451874367No Hit
GTCGTGACTGGGAAAACCCTGGTCAGCTGTCGTGATTGTGAGTTGCAGTG270.19539730785931392No Hit
GTCGTGACTGGGAAAACCCTGGAGCCGCGTCGTGATACCGATGGGCAGTG270.19539730785931392No Hit
GTCGTGACTGGGAAAACCCTGGCTGCATGTCGTGATATCAACCGTCAGTG270.19539730785931392No Hit
GTCGTGACTGGGAAAACCCTGGGCGAACGTCGTGATAACGTTCCCCAGTG260.18816037053119122No Hit
GTCGTGACTGGGAAAACCCTGGAGCTCGGTCGTGATTTTCTCGTGACAGT260.18816037053119122No Hit
GTCGTGACTGGGAAAACCCTGGGTTGGGGTCGTGATGTAAGGGAACAGTG260.18816037053119122No Hit
GTCGTGACTGGGAAAACCCTGGAGCCATGTCGTGATTCAGTGGAGACAGT260.18816037053119122No Hit
GTCGTGACTGGGAAAACCCTGGCAATGTGTCGTGATGACTGTTTCACAGT260.18816037053119122No Hit
GTCGTGACTGGGAAAACCCTGGGCATGGGTCGTGATTGTCCGTGACAGTG250.18092343320306847No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGCGTCGTGATGCCCGCGTTCAGTG250.18092343320306847No Hit
GTCGTGACTGGGAAAACCCTGGCCAACTGTCGTGATAGCGCGCAGCAGTG250.18092343320306847No Hit
GTCGTGACTGGGAAAACCCTGGGCAAAGGTCGTGATCAGGTTTACACAGT240.17368649587494572No Hit
GTCGTGACTGGGAAAACCCTGGTCCTCAGTCGTGATTCGGTTGATACAGT240.17368649587494572No Hit
GTCGTGACTGGGAAAACCCTGGGCTCTCGTCGTGATGCACGTGATCAGTG240.17368649587494572No Hit
GTCGTGACTGGGAAAACCCTGGGGCATGGTCGTGATAATTGCAAACAGTG230.166449558546823No Hit
GTCGTGACTGGGAAAACCCTGGATCGCAGTCGTGATGTTAATGAGCAGTG230.166449558546823No Hit
GTCGTGACTGGGAAAACCCTGGTCCCCTGTCGTGATAAGGAAAGCACAGT230.166449558546823No Hit
GTCGTGACTGGGAAAACCCTGGGAACCTGTCGTGATTGAGAATTGACAGT230.166449558546823No Hit
GTCGTGACTGGGAAAACCCTGGCGCGCTGTCGTGATGCGTACTTGACAGT230.166449558546823No Hit
GTCGTGACTGGGAAAACCCTGGGCACACGTCGTGATCAATAGGGGACAGT220.15921262121870025No Hit
GTCGTGACTGGGAAAACCCTGGCCAATTGTCGTGATGTGTCTTACTCGGT220.15921262121870025No Hit
GTCGTGACTGGGAAAACCCTGGAACCGCGTCGTGATTAGTGTTCCCAGTG220.15921262121870025No Hit
GTCGTGACTGGGAAAACCCTGGACAGAGGTCGTGATCGCGAGAGTACAGT220.15921262121870025No Hit
GTCGTGACTGGGAAAACCCTGGCGTTCAGTCGTGATAATCGTTTGATGGT210.1519756838905775No Hit
GTCGTGACTGGGAAAACCCTGGAAGTGTGTCGTGATTTACGCATCCAGTG210.1519756838905775No Hit
GTCGTGACTGGGAAAACCCTGGGCGTCTGTCGTGATTCCAATGCCTCGGT210.1519756838905775No Hit
GTCGTGACTGGGAAAACCCTGGATCAGTGTCGTGATACTAAGTAGACAGT200.14473874656245478No Hit
GTCGTGACTGGGAAAACCCTGGCAGCTAGTCGTGATCAAGCCTCAACAGT200.14473874656245478No Hit
GTCGTGACTGGGAAAACCCTGGTCGCTTGTCGTGATCATGTCCGAACAGT200.14473874656245478No Hit
GTCGTGACTGGGAAAACCCTGGGTCTATGTCGTGATGATTGTACCCAGTG190.13750180923433203No Hit
GTCGTGACTGGGAAAACCCTGGGGTCACGTCGTGATATGCAGTAGACAGT190.13750180923433203No Hit
GTCGTGACTGGGAAAACCCTGGGACCGGGTCGTGATTCGTAATGCCAGTG180.13026487190620928No Hit
GTCGTGACTGGGAAAACCCTGGCATCGAGTCGTGATCAACATGACCAGTG180.13026487190620928No Hit
GTCGTGACTGGGAAAACCCTGGGAACGGGTCGTGATGCTAATGAGCAGTG180.13026487190620928No Hit
GTCGTGACTGGGAAAACCCTGGAGCAAGGTCGTGATACTAGGGATCAGTG180.13026487190620928No Hit
GTCGTGACTGGGAAAACCCTGGGCCAGAGTCGTGATGTCGGGGTCCAGTG170.12302793457808654No Hit
GTCGTGACTGGGAAAACCCTGGGCGCCAGTCGTGATCAAAGCAAACAGTG170.12302793457808654No Hit
GTCGTGACTGGGAAAACCCTGGGAATGGGTCGTGATATACATCGAACAGT170.12302793457808654No Hit
GTCGTGACTGGGAAAACCCTGGGACACAGTCGTGATTAGAACTTTCAGTG170.12302793457808654No Hit
GTCGTGACTGGGAAAACCCTGGCGCGGGGTCGTGATCCGTCCCAGCAGTG170.12302793457808654No Hit
GTCGTGACTGGGAAAACCCTGGCCATGAGTCGTGATCGTTAGTTTCAGTG170.12302793457808654No Hit
GTCGTGACTGGGAAAACCCTGGGATAAGGTCGTGTCTAAAGTCTAATGGT170.12302793457808654No Hit
GTCGTGACTGGGAAAACCCTGGGTAGCCGTCGTGATCTGGGGAGCCAGTG170.12302793457808654No Hit
GTCGTGACTGGGAAAACCCTGGGCCGAAGTCGTGATAACTATATCACAGT160.1157909972499638No Hit
GTCGTGACTGGGAAAACCCTGGATGAGCGTCGTGATCGTTGCTACACAGT160.1157909972499638No Hit
GTCGTGACTGGGAAAACCCTGGAGATTGGTCGTGATGGCATCGTGCAGTG160.1157909972499638No Hit
GTCGTGACTGGGAAAACCCTGGCAGCGGGTCGTGATATCAAATACACAGT160.1157909972499638No Hit
GTCGTGACTGGGAAAACCCTGGACTCAGGTCGTGATAGTCTCCCTCAGTG160.1157909972499638No Hit
GTCGTGACTGGGAAAACCCTGGCAGCTAGTCGTGATGTAGATGGTCAGTG160.1157909972499638No Hit
GTCGTGACTGGGAAAACCCTGGAAATCCGTCGTGATGTGAGGGACCAGTG160.1157909972499638No Hit
GTCGTGACTGGGAAAACCCTGGAGTATGGTCGTGATCCTGCTAAAACAGT160.1157909972499638No Hit
GTCGTGACTGGGAAAACCCTGGTACCCCGTCGTGATTTAGAGTCTCAGTG160.1157909972499638No Hit
GTCGTGACTGGGAAAACCCTGGCTGCGTGTCGTGATCGGGACGACACAGT160.1157909972499638No Hit
GTCGTGACTGGGAAAACCCTGGATTTTCGTCGTGATATCCGAGTGCAGTG150.10855405992184108No Hit
GTCGTGACTGGGAAAACCCTGGGTCCACGTCGTGATTTACATGGACAGTG150.10855405992184108No Hit
GTCGTGACTGGGAAAACCCTGGCTTGCGGTCGTGATAGAACATAGCAGTG150.10855405992184108No Hit
GTCGTGACTGGGAAAACCCTGGCCTCTAGTCGTGATAGTAAGTTTCAGTG150.10855405992184108No Hit
GTCGTGACTGGGAAAACCCTGGGCTGCCGTCGTGATCGAGAAGCCCAGTG150.10855405992184108No Hit
GTCGTGACTGGGAAAACCCTGGATCTTCGTCGTGATTTGGTCGCTACAGT150.10855405992184108No Hit
GTCGTGACTGGGAAAACCCTGGATATGGGTCGTGATCCATTTACCCAGTG150.10855405992184108No Hit
GTCGTGACTGGGAAAACCCTGGAACTCTGTCGTGATACTGTTCCCCAGTG150.10855405992184108No Hit
GTCGTGACTGGGAAAACCCTGGCTTAGCGTCGTGATTAATGGGAGCAGTG150.10855405992184108No Hit
GTCGTGACTGGGAAAACCCTGGGCGGAGTCGTGATCCCACGATGGACAGT140.10131712259371835No Hit
GTCGTGACTGGGAAAACCCTGGCTCCATGTCGTGATGTCCTGGGCCAGTG140.10131712259371835No Hit
GTCGTGACTGGGAAAACCCTGGAACCGCGTCGTGATTTGCTTGAGCAGTG140.10131712259371835No Hit
GTCGTGACTGGGAAAACCCTGGGATTCGGTCGTGTACCTGGTGAAACAGT140.10131712259371835No Hit
GTCGTGACTGGGAAAACCCTGGAACCACGTCGTGATTGTTGCTCCCAGTG140.10131712259371835No Hit
GTCGTGACTGGGAAAACCCTGGCAGTGCGTCGTGATGGCCCGACCACAGT140.10131712259371835No Hit
GTCGTGACTGGGAAAACCCTGGGGCCGCGTCGTGATCTCTGTGCCCAGTG140.10131712259371835No Hit
GTCGTGACTGGGAAAACCCTGGTACCTAGTCGTGATTCAGAGTTGCAGTG140.10131712259371835No Hit
GTCGTGACTGGGAAAACCCTGGCAACTTGTCGTGATCCTATCAAGACAGT140.10131712259371835No Hit
GTCGTGACTGGGAAAACCCTGGAACCCGGTCGTGATAGTGTAGTAACAGT140.10131712259371835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTGA100.0014668631244.63371
TCGTGGC151.5178062E-5243.740882
CGTGGCT151.540154E-5242.854543
ACTGGGA11350.0241.974647
TATTAAA100.0015158346241.974629
TCGTGAC11350.0240.519642
GTGACTG11400.0239.659094
TGACTGG11450.0238.804675
GACTGGG11550.0237.784596
TGGGAAA11400.0237.729469
CGTGACT11500.0236.519213
CTGGGAA11800.0234.797428
GTCGTGA21750.0124.2851641
GTCGTGG354.3768127E-4104.843011
GGACTTC150.001259202380.65821240-242
ACGCAGC600.080.65821240-242
CGCAGCC550.080.65821240-242
CGAAATT203.883395E-580.65821240-242
TCCGAAA203.883395E-580.65821240-242
GGAGTGA150.001259202380.65821240-242
Produced by FastQC (version 0.11.5)